T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS
Identifieur interne : 005F07 ( Main/Merge ); précédent : 005F06; suivant : 005F08T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS
Auteurs : Yue-Dan Wang [République populaire de Chine, Hong Kong] ; Wan-Yee Fion Sin [Hong Kong] ; Guo-Bing Xu [République populaire de Chine] ; Huang-Hua Yang [Danemark] ; Tin-Yau Wong [Danemark] ; Xue-Wen Pang [République populaire de Chine] ; Xiao-Yan He [République populaire de Chine] ; Hua-Gang Zhang [République populaire de Chine] ; JOICE NA LEE NG [Danemark] ; Chak-Sum Samuel Cheng [Hong Kong] ; JING JU [Hong Kong] ; LI MENG [Hong Kong] ; Rui-Feng Yang [République populaire de Chine] ; Sik-To Lai [Danemark] ; Zhi-Hong Guo [Danemark] ; YONG XIE [Hong Kong] ; Wei-Feng Chen [République populaire de Chine]Source :
- Journal of virology [ 0022-538X ] ; 2004.
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Abstract
The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-γ) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-γ-secreting T-cell response in HLA-A2+ donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2+ healthy donors or in HLA-A2- donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2+ SARS-CoV-infected patients.
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type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Yang, Huang Hua" sort="Yang, Huang Hua" uniqKey="Yang H" first="Huang-Hua" last="Yang">Huang-Hua Yang</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Pang, Xue Wen" sort="Pang, Xue Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Jing Ju" sort="Jing Ju" uniqKey="Jing Ju" last="Jing Ju">JING JU</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Li Meng" sort="Li Meng" uniqKey="Li Meng" last="Li Meng">LI MENG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Rui Feng" sort="Yang, Rui Feng" uniqKey="Yang R" first="Rui-Feng" last="Yang">Rui-Feng Yang</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>First Hospital, Peking University</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Zhi Hong" sort="Guo, Zhi Hong" uniqKey="Guo Z" first="Zhi-Hong" last="Guo">Zhi-Hong Guo</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0314703</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0314703 INIST</idno><idno type="RBID">Pascal:04-0314703</idno><idno type="wicri:Area/PascalFrancis/Corpus">000879</idno><idno type="wicri:Area/PascalFrancis/Curation">000111</idno><idno 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type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Sin, Wan Yee Fion" sort="Sin, Wan Yee Fion" uniqKey="Sin W" first="Wan-Yee Fion" last="Sin">Wan-Yee Fion Sin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Guo Bing" sort="Xu, Guo Bing" uniqKey="Xu G" first="Guo-Bing" last="Xu">Guo-Bing Xu</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>First Hospital, Peking University</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Yang, Huang Hua" sort="Yang, Huang Hua" uniqKey="Yang H" first="Huang-Hua" last="Yang">Huang-Hua Yang</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Pang, Xue Wen" sort="Pang, Xue Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Jing Ju" sort="Jing Ju" uniqKey="Jing Ju" last="Jing Ju">JING JU</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Li Meng" sort="Li Meng" uniqKey="Li Meng" last="Li Meng">LI MENG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Rui Feng" sort="Yang, Rui Feng" uniqKey="Yang R" first="Rui-Feng" last="Yang">Rui-Feng Yang</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>First Hospital, Peking University</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Zhi Hong" sort="Guo, Zhi Hong" uniqKey="Guo Z" first="Zhi-Hong" last="Guo">Zhi-Hong Guo</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term><term>Cellular immunity</term><term>Coronavirus</term><term>Human</term><term>Microbiology</term><term>Protein A</term><term>Severe acute respiratory syndrome</term><term>T-Lymphocyte</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Homme</term><term>Lymphocyte T</term><term>Déterminant antigénique</term><term>Protéine A</term><term>Immunité cellulaire</term><term>Microbiologie</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-γ) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-γ-secreting T-cell response in HLA-A2<sup>+</sup> donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2<sup>+</sup> healthy donors or in HLA-A2- donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2<sup>+</sup> SARS-CoV-infected patients.</div></front></TEI><affiliations><list><country><li>Danemark</li><li>Hong Kong</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></noRegion><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><name sortKey="Pang, Xue Wen" sort="Pang, Xue Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><name sortKey="Xu, Guo Bing" sort="Xu, Guo Bing" uniqKey="Xu G" first="Guo-Bing" last="Xu">Guo-Bing Xu</name><name sortKey="Yang, Rui Feng" sort="Yang, Rui Feng" uniqKey="Yang R" first="Rui-Feng" last="Yang">Rui-Feng Yang</name><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name></country><country name="Hong Kong"><noRegion><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></noRegion><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name><name sortKey="Jing Ju" sort="Jing Ju" uniqKey="Jing Ju" last="Jing Ju">JING JU</name><name sortKey="Li Meng" sort="Li Meng" uniqKey="Li Meng" last="Li Meng">LI MENG</name><name sortKey="Sin, Wan Yee Fion" sort="Sin, Wan Yee Fion" uniqKey="Sin W" first="Wan-Yee Fion" last="Sin">Wan-Yee Fion Sin</name><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name></country><country name="Danemark"><noRegion><name sortKey="Yang, Huang Hua" sort="Yang, Huang Hua" uniqKey="Yang H" first="Huang-Hua" last="Yang">Huang-Hua Yang</name></noRegion><name sortKey="Guo, Zhi Hong" sort="Guo, Zhi Hong" uniqKey="Guo Z" first="Zhi-Hong" last="Guo">Zhi-Hong Guo</name><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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