SrasV1, Main, Merge, bibRecord, 005F02

The life cycle of SARS coronavirus in vero E6 cells

Identifieur interne : 005F02 ( Main/Merge ); précédent : 005F01; suivant : 005F03

The life cycle of SARS coronavirus in vero E6 cells

Auteurs : ZHANG QINFEN [République populaire de Chine] ; CUI JINMING [République populaire de Chine] ; HUANG XIAOJUN [République populaire de Chine] ; ZHENG HUANYING [République populaire de Chine] ; HUANG JICHENG [République populaire de Chine] ; FANG LING [République populaire de Chine] ; LI KUNPENG [République populaire de Chine] ; ZHANG JINGQIANG [République populaire de Chine]

Source :

Journal of medical virology [ 0146-6615 ] ; 2004.

RBID : Pascal:04-0418172

Descripteurs français

Pascal (Inist)
- Coronavirus, Cycle développement, Microscopie électronique, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Electron microscopy, Life cycle, Severe acute respiratory syndrome.

Abstract

The aim ofthe study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.

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Pascal:04-0418172

Le document en format XML

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<author><name sortKey="Zhang Jingqiang" sort="Zhang Jingqiang" uniqKey="Zhang Jingqiang" last="Zhang Jingqiang">ZHANG JINGQIANG</name>
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<author><name sortKey="Huang Jicheng" sort="Huang Jicheng" uniqKey="Huang Jicheng" last="Huang Jicheng">HUANG JICHENG</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Center for Disease Control and Prevention of Guangdong Province</s1>
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<author><name sortKey="Fang Ling" sort="Fang Ling" uniqKey="Fang Ling" last="Fang Ling">FANG LING</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Center for Disease Control and Prevention of Guangdong Province</s1>
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<author><name sortKey="Li Kunpeng" sort="Li Kunpeng" uniqKey="Li Kunpeng" last="Li Kunpeng">LI KUNPENG</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>State Key Lab for Biocontrol, Zhongshan University</s1>
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</author>
<author><name sortKey="Zhang Jingqiang" sort="Zhang Jingqiang" uniqKey="Zhang Jingqiang" last="Zhang Jingqiang">ZHANG JINGQIANG</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>State Key Lab for Biocontrol, Zhongshan University</s1>
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<series><title level="j" type="main">Journal of medical virology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Electron microscopy</term>
<term>Life cycle</term>
<term>Severe acute respiratory syndrome</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
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<front><div type="abstract" xml:lang="en">The aim ofthe study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
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<settlement><li>Jiangmen</li>
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<tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhang Qinfen" sort="Zhang Qinfen" uniqKey="Zhang Qinfen" last="Zhang Qinfen">ZHANG QINFEN</name>
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<name sortKey="Cui Jinming" sort="Cui Jinming" uniqKey="Cui Jinming" last="Cui Jinming">CUI JINMING</name>
<name sortKey="Fang Ling" sort="Fang Ling" uniqKey="Fang Ling" last="Fang Ling">FANG LING</name>
<name sortKey="Huang Jicheng" sort="Huang Jicheng" uniqKey="Huang Jicheng" last="Huang Jicheng">HUANG JICHENG</name>
<name sortKey="Huang Xiaojun" sort="Huang Xiaojun" uniqKey="Huang Xiaojun" last="Huang Xiaojun">HUANG XIAOJUN</name>
<name sortKey="Li Kunpeng" sort="Li Kunpeng" uniqKey="Li Kunpeng" last="Li Kunpeng">LI KUNPENG</name>
<name sortKey="Zhang Jingqiang" sort="Zhang Jingqiang" uniqKey="Zhang Jingqiang" last="Zhang Jingqiang">ZHANG JINGQIANG</name>
<name sortKey="Zheng Huanying" sort="Zheng Huanying" uniqKey="Zheng Huanying" last="Zheng Huanying">ZHENG HUANYING</name>
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The life cycle of SARS coronavirus in vero E6 cells

The life cycle of SARS coronavirus in vero E6 cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri