The life cycle of SARS coronavirus in vero E6 cells
Identifieur interne : 005F02 ( Main/Merge ); précédent : 005F01; suivant : 005F03The life cycle of SARS coronavirus in vero E6 cells
Auteurs : ZHANG QINFEN [République populaire de Chine] ; CUI JINMING [République populaire de Chine] ; HUANG XIAOJUN [République populaire de Chine] ; ZHENG HUANYING [République populaire de Chine] ; HUANG JICHENG [République populaire de Chine] ; FANG LING [République populaire de Chine] ; LI KUNPENG [République populaire de Chine] ; ZHANG JINGQIANG [République populaire de Chine]Source :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The aim ofthe study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.
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Pascal:04-0418172Le document en format XML
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<front><div type="abstract" xml:lang="en">The aim ofthe study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.</div>
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