Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein.
Identifieur interne : 005824 ( Main/Merge ); précédent : 005823; suivant : 005825Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein.
Auteurs : Yue-Dan Wang [République populaire de Chine] ; Wei Feng ChenSource :
- Clinical immunology (Orlando, Fla.) [ 1521-6616 ] ; 2004.
Descripteurs français
- KwdFr :
- Acide iotalamique (analogues et dérivés), Animaux, Antigène HLA-A2 (immunologie), Cytométrie en flux, Déterminants antigéniques des lymphocytes T (immunologie), Humains, Immunoglobulines (immunologie), Interféron gamma (immunologie), Lymphocytes T cytotoxiques (immunologie), Méglumine, Protéines de fusion virale, Syndrome respiratoire aigu sévère (immunologie), Virus du SRAS (immunologie).
- MESH :
- analogues et dérivés : Acide iotalamique.
- immunologie : Antigène HLA-A2, Déterminants antigéniques des lymphocytes T, Immunoglobulines, Interféron gamma, Lymphocytes T cytotoxiques, Syndrome respiratoire aigu sévère, Virus du SRAS.
- Animaux, Cytométrie en flux, Humains, Méglumine, Protéines de fusion virale.
English descriptors
- KwdEn :
- Animals, Epitopes, T-Lymphocyte (immunology), Flow Cytometry, HLA-A2 Antigen (immunology), Humans, Immunoglobulins (immunology), Interferon-gamma (immunology), Iothalamic Acid (analogs & derivatives), Meglumine, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes, Cytotoxic (immunology), Viral Fusion Proteins.
- MESH :
- chemical , analogs & derivatives : Iothalamic Acid.
- chemical , immunology : Epitopes, T-Lymphocyte, HLA-A2 Antigen, Immunoglobulins, Interferon-gamma.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- Animals, Flow Cytometry, Humans, Meglumine, Viral Fusion Proteins.
Abstract
To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2+ SARS-recovered donors at different time points post disease. CD8+DimerX-S1203+ CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8+DimerX-S1203+ cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus.
DOI: 10.1016/j.clim.2004.07.004
PubMed: 15451471
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pubmed:15451471Le document en format XML
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specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein.</title><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China. wangyuedan@hotmail.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei Feng" last="Chen">Wei Feng Chen</name></author></analytic><series><title level="j">Clinical immunology (Orlando, Fla.)</title><idno type="ISSN">1521-6616</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Flow Cytometry</term><term>HLA-A2 Antigen (immunology)</term><term>Humans</term><term>Immunoglobulins (immunology)</term><term>Interferon-gamma (immunology)</term><term>Iothalamic Acid (analogs & derivatives)</term><term>Meglumine</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide iotalamique (analogues et dérivés)</term><term>Animaux</term><term>Antigène HLA-A2 (immunologie)</term><term>Cytométrie en flux</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Humains</term><term>Immunoglobulines (immunologie)</term><term>Interféron gamma (immunologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Méglumine</term><term>Protéines de fusion virale</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Iothalamic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>HLA-A2 Antigen</term><term>Immunoglobulins</term><term>Interferon-gamma</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide iotalamique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigène HLA-A2</term><term>Déterminants antigéniques des lymphocytes T</term><term>Immunoglobulines</term><term>Interféron gamma</term><term>Lymphocytes T cytotoxiques</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Flow Cytometry</term><term>Humans</term><term>Meglumine</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cytométrie en flux</term><term>Humains</term><term>Méglumine</term><term>Protéines de fusion virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2+ SARS-recovered donors at different time points post disease. CD8+DimerX-S1203+ CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8+DimerX-S1203+ cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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