Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
Identifieur interne : 005720 ( Main/Merge ); précédent : 005719; suivant : 005721Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
Auteurs : Baomei Wang [République populaire de Chine] ; Huabiao Chen ; Xiaodong Jiang ; Minghui Zhang ; Tao Wan ; Nan Li ; Xiangyang Zhou ; Yanfeng Wu ; Feng Yang ; Yizhi Yu ; Xiaoning Wang ; Ruifu Yang ; Xuetao CaoSource :
- Blood [ 0006-4971 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Antigène HLA-A2, Antigènes HLA-A (), Antigènes HLA-A (génétique), Antigènes HLA-A (immunologie), Antigènes HLA-A (métabolisme), Déterminants antigéniques des lymphocytes T (immunologie), Fragments peptidiques (génétique), Fragments peptidiques (immunologie), Fragments peptidiques (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Interféron gamma (biosynthèse), Lignée cellulaire, Lymphocytes T CD8+ (immunologie), Lymphocytes T cytotoxiques (immunologie), Lymphocytes T cytotoxiques (métabolisme), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines virales structurales (génétique), Protéines virales structurales (immunologie), Rate (cytologie), Souris, Souris transgéniques, Séquence d'acides aminés, Transduction génétique, Virus du SRAS (), Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Interféron gamma.
- cytologie : Rate.
- génétique : Antigènes HLA-A, Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales structurales.
- immunologie : Antigènes HLA-A, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Lymphocytes T CD8+, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Protéines virales structurales, Virus du SRAS.
- métabolisme : Antigènes HLA-A, Fragments peptidiques, Lymphocytes T cytotoxiques.
- Animaux, Antigène HLA-A2, Antigènes HLA-A, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Souris, Souris transgéniques, Séquence d'acides aminés, Transduction génétique, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, CD8-Positive T-Lymphocytes (immunology), Cell Line, Epitopes, T-Lymphocyte (immunology), HLA-A Antigens (chemistry), HLA-A Antigens (genetics), HLA-A Antigens (immunology), HLA-A Antigens (metabolism), HLA-A2 Antigen, Humans, Interferon-gamma (biosynthesis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Transgenic, Peptide Fragments (genetics), Peptide Fragments (immunology), Peptide Fragments (metabolism), SARS Virus (chemistry), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Spleen (cytology), T-Lymphocytes, Cytotoxic (immunology), T-Lymphocytes, Cytotoxic (metabolism), Transduction, Genetic, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Structural Proteins (genetics), Viral Structural Proteins (immunology).
- MESH :
- chemical , biosynthesis : Interferon-gamma.
- chemical , chemistry : HLA-A Antigens.
- chemical , genetics : HLA-A Antigens, Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins, Viral Structural Proteins.
- chemical , immunology : Epitopes, T-Lymphocyte, HLA-A Antigens, Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins, Viral Structural Proteins.
- chemistry : SARS Virus.
- cytology : Spleen.
- immunology : CD8-Positive T-Lymphocytes, SARS Virus, T-Lymphocytes, Cytotoxic.
- chemical , metabolism : HLA-A Antigens, Peptide Fragments, T-Lymphocytes, Cytotoxic.
- Amino Acid Sequence, Animals, Cell Line, HLA-A2 Antigen, Humans, Mice, Mice, Transgenic, Spike Glycoprotein, Coronavirus, Transduction, Genetic.
Abstract
A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Peptides with high affinity for HLA-A*0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2.1/K(b) transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) harvested from healthy HLA-A2.1(+) donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon-gamma (IFN-gamma) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specific CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A*0201-SSp-1 tetramer staining revealed the presence of significant populations of SSp-1-specific CTLs in SSp-1-induced CD8(+) T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS.
DOI: 10.1182/blood-2003-11-4072
PubMed: 15016646
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pubmed:15016646Le document en format XML
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sort="Zhang, Minghui" uniqKey="Zhang M" first="Minghui" last="Zhang">Minghui Zhang</name></author><author><name sortKey="Wan, Tao" sort="Wan, Tao" uniqKey="Wan T" first="Tao" last="Wan">Tao Wan</name></author><author><name sortKey="Li, Nan" sort="Li, Nan" uniqKey="Li N" first="Nan" last="Li">Nan Li</name></author><author><name sortKey="Zhou, Xiangyang" sort="Zhou, Xiangyang" uniqKey="Zhou X" first="Xiangyang" last="Zhou">Xiangyang Zhou</name></author><author><name sortKey="Wu, Yanfeng" sort="Wu, Yanfeng" uniqKey="Wu Y" first="Yanfeng" last="Wu">Yanfeng Wu</name></author><author><name sortKey="Yang, Feng" sort="Yang, Feng" uniqKey="Yang F" first="Feng" last="Yang">Feng Yang</name></author><author><name sortKey="Yu, Yizhi" sort="Yu, Yizhi" uniqKey="Yu Y" first="Yizhi" last="Yu">Yizhi Yu</name></author><author><name sortKey="Wang, Xiaoning" sort="Wang, Xiaoning" uniqKey="Wang X" first="Xiaoning" last="Wang">Xiaoning Wang</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author><author><name sortKey="Cao, Xuetao" sort="Cao, Xuetao" uniqKey="Cao X" first="Xuetao" last="Cao">Xuetao Cao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15016646</idno><idno type="pmid">15016646</idno><idno type="doi">10.1182/blood-2003-11-4072</idno><idno type="wicri:Area/PubMed/Corpus">002F22</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F22</idno><idno type="wicri:Area/PubMed/Curation">002F22</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F22</idno><idno type="wicri:Area/PubMed/Checkpoint">002C64</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C64</idno><idno type="wicri:Area/Ncbi/Merge">000650</idno><idno type="wicri:Area/Ncbi/Curation">000650</idno><idno 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Chen</name></author><author><name sortKey="Jiang, Xiaodong" sort="Jiang, Xiaodong" uniqKey="Jiang X" first="Xiaodong" last="Jiang">Xiaodong Jiang</name></author><author><name sortKey="Zhang, Minghui" sort="Zhang, Minghui" uniqKey="Zhang M" first="Minghui" last="Zhang">Minghui Zhang</name></author><author><name sortKey="Wan, Tao" sort="Wan, Tao" uniqKey="Wan T" first="Tao" last="Wan">Tao Wan</name></author><author><name sortKey="Li, Nan" sort="Li, Nan" uniqKey="Li N" first="Nan" last="Li">Nan Li</name></author><author><name sortKey="Zhou, Xiangyang" sort="Zhou, Xiangyang" uniqKey="Zhou X" first="Xiangyang" last="Zhou">Xiangyang Zhou</name></author><author><name sortKey="Wu, Yanfeng" sort="Wu, Yanfeng" uniqKey="Wu Y" first="Yanfeng" last="Wu">Yanfeng Wu</name></author><author><name sortKey="Yang, Feng" sort="Yang, Feng" uniqKey="Yang F" first="Feng" last="Yang">Feng Yang</name></author><author><name sortKey="Yu, Yizhi" sort="Yu, Yizhi" uniqKey="Yu Y" first="Yizhi" last="Yu">Yizhi Yu</name></author><author><name sortKey="Wang, Xiaoning" sort="Wang, Xiaoning" uniqKey="Wang X" first="Xiaoning" last="Wang">Xiaoning Wang</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author><author><name sortKey="Cao, Xuetao" sort="Cao, Xuetao" uniqKey="Cao X" first="Xuetao" last="Cao">Xuetao Cao</name></author></analytic><series><title level="j">Blood</title><idno type="ISSN">0006-4971</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>Cell Line</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>HLA-A Antigens (chemistry)</term><term>HLA-A Antigens (genetics)</term><term>HLA-A Antigens (immunology)</term><term>HLA-A Antigens (metabolism)</term><term>HLA-A2 Antigen</term><term>Humans</term><term>Interferon-gamma (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Transgenic</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (immunology)</term><term>Peptide Fragments (metabolism)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spleen (cytology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>T-Lymphocytes, Cytotoxic (metabolism)</term><term>Transduction, Genetic</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Structural Proteins (genetics)</term><term>Viral Structural Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A ()</term><term>Antigènes HLA-A (génétique)</term><term>Antigènes HLA-A (immunologie)</term><term>Antigènes HLA-A (métabolisme)</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (immunologie)</term><term>Fragments peptidiques (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Interféron gamma (biosynthèse)</term><term>Lignée cellulaire</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Lymphocytes T cytotoxiques (métabolisme)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines virales structurales (génétique)</term><term>Protéines virales structurales (immunologie)</term><term>Rate (cytologie)</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Transduction génétique</term><term>Virus du SRAS ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>HLA-A Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>HLA-A Antigens</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>HLA-A Antigens</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Rate</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes HLA-A</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes HLA-A</term><term>Déterminants antigéniques des lymphocytes T</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Lymphocytes T CD8+</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD8-Positive T-Lymphocytes</term><term>SARS Virus</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>HLA-A Antigens</term><term>Peptide Fragments</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes HLA-A</term><term>Fragments peptidiques</term><term>Lymphocytes T cytotoxiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>HLA-A2 Antigen</term><term>Humans</term><term>Mice</term><term>Mice, Transgenic</term><term>Spike Glycoprotein, Coronavirus</term><term>Transduction, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Transduction génétique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Peptides with high affinity for HLA-A*0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2.1/K(b) transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) harvested from healthy HLA-A2.1(+) donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon-gamma (IFN-gamma) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specific CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A*0201-SSp-1 tetramer staining revealed the presence of significant populations of SSp-1-specific CTLs in SSp-1-induced CD8(+) T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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