SrasV1, Main, Merge, bibRecord, 005231

An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

Identifieur interne : 005231 ( Main/Merge ); précédent : 005230; suivant : 005232

An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

Auteurs : Parviz Ashtari [République populaire de Chine] ; XIAOXIAO HE [République populaire de Chine] ; KEMIN WANG [République populaire de Chine] ; PING GONG [République populaire de Chine]

Source :

Talanta : (Oxford) [ 0039-9140 ] ; 2005.

RBID : Pascal:06-0195089

Descripteurs français

Pascal (Inist)
- Nanoparticule, Microémulsion, Immobilisation, Liaison covalente, Efficacité, Sélectivité, Silice, Eau, Fer oxyde, DNA.
Wicri :
- topic : Eau.

English descriptors

KwdEn :
- Covalent bond, DNA, Efficiency, Immobilization, Iron oxide, Microemulsion, Nanoparticle, Selectivity, Silica, Water.

Abstract

In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.

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Pascal:06-0195089

Le document en format XML

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<front><div type="abstract" xml:lang="en">In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.</div>
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An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri