SrasV1, Main, Merge, bibRecord, 005182

Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

Identifieur interne : 005182 ( Main/Merge ); précédent : 005181; suivant : 005183

Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

Auteurs : S. Chen [République populaire de Chine] ; D. Lu [République populaire de Chine] ; M. Zhang [République populaire de Chine] ; J. Che [République populaire de Chine] ; Z. Yin [République populaire de Chine] ; S. Zhang [République populaire de Chine] ; W. Zhang [République populaire de Chine] ; X. Bo [République populaire de Chine] ; Y. Ding [République populaire de Chine] ; S. Wang [République populaire de Chine]

Source :

European journal of clinical microbiology & infectious diseases : (Print) [ 0934-9723 ] ; 2005.

RBID : Pascal:05-0440639

Descripteurs français

Pascal (Inist)
- Syndrome respiratoire aigu sévère, Antigène, Anticorps, Sérum, Coronavirus, Homme.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antibody, Antigen, Coronavirus, Human, Serum, Severe acute respiratory syndrome.

Abstract

The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.</div>
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Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

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