Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study
Identifieur interne : 005170 ( Main/Merge ); précédent : 005169; suivant : 005171Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study
Auteurs : Chia-Wei Lai [Taïwan] ; Yao-Chi Chung [Taïwan] ; Yiu-Kay Lai [Taïwan] ; Margaret Dah-Tsyr Chang [Taïwan] ; Yu-Chen Hu [Taïwan]Source :
- Biotechnology letters [ 0141-5492 ] ; 2005.
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Abstract
Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
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<term>Immobilized metal affinity chromatography</term>
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<front><div type="abstract" xml:lang="en">Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.</div>
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<affiliations><list><country><li>Taïwan</li>
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<tree><country name="Taïwan"><noRegion><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
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<name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
<name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
<name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
<name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
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