SrasV1, Main, Merge, bibRecord, 005082

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Identifieur interne : 005082 ( Main/Merge ); précédent : 005081; suivant : 005083

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Auteurs : K. Hattermann [Allemagne] ; M. A. Müller [Allemagne] ; A. Nitsche [Allemagne] ; S. Wendt [Allemagne] ; O. Donoso Mantke [Allemagne] ; M. Niedrig [Allemagne]

Source :

Archives of virology [ 0304-8608 ] ; 2005.

RBID : Pascal:05-0484477

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Sensibilité, Lignée cellulaire, Pouvoir infectant.

English descriptors

KwdEn :
- Cell line, Infectivity, Sensitivity, Severe acute respiratory syndrome virus.

Abstract

In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS-Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.

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Pascal:05-0484477

Le document en format XML

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<front><div type="abstract" xml:lang="en">In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS-Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.</div>
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Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri