A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARS-CoV-neutralizing antibodies
Identifieur interne : 004C70 ( Main/Merge ); précédent : 004C69; suivant : 004C71A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARS-CoV-neutralizing antibodies
Auteurs : Milosz Faber ; Elaine W. Lamirande [États-Unis] ; Anjeanette Roberts [États-Unis] ; Amy B. Rice ; Hilary Koprowski ; Bernhard Dietzschold ; Matthias J. Schnell [États-Unis]Source :
- The Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Antigènes viraux (génétique), DNA-directed RNA polymerases (génétique), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines (génétique), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Modèles animaux, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines virales (génétique), Rhabdoviridae (génétique), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Tests de neutralisation, Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins synthétiques (génétique), Vaccins synthétiques (immunologie), Vecteurs génétiques, Virus du SRAS (immunologie).
- MESH :
- génétique : Antigènes viraux, DNA-directed RNA polymerases, Glycoprotéines, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines virales, Rhabdoviridae, Vaccins antiviraux, Vaccins synthétiques.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Vaccins antiviraux, Vaccins synthétiques, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Modèles animaux, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Tests de neutralisation, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antigens, Viral (genetics), DNA-Directed RNA Polymerases (genetics), Female, Genetic Vectors, Glycoproteins (genetics), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Models, Animal, Neutralization Tests, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Rhabdoviridae (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Proteins (genetics), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Antigens, Viral, DNA-Directed RNA Polymerases, Glycoproteins, Membrane Glycoproteins, Nucleocapsid Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Proteins, Viral Vaccines.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- genetics : Rhabdoviridae.
- immunology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Female, Genetic Vectors, Mice, Mice, Inbred BALB C, Models, Animal, Neutralization Tests, Spike Glycoprotein, Coronavirus.
Abstract
Foreign viral proteins expressed by rabies virus (RV) have been shown to induce potent humoral and cellular immune responses in immunized animals. In addition, highly attenuated and, therefore, very safe RV-based vectors have been constructed. Here, an RV-based vaccine vehicle was utilized as a novel vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). For this approach, the SARS-CoV nucleocapsid protein (N) or envelope spike protein (S) genes were cloned between the RV glycoprotein G and polymerase L genes. Recombinant vectors expressing SARS-CoV N or S protein were recovered and their immunogenicity was studied in mice. A single inoculation with the RV-based vaccine expressing SARS-CoV S protein induced a strong SARS-CoV-neutralizing antibody response. The ability of the RV-SARS-CoV S vector to confer immunity after a single inoculation makes this live vaccine a promising candidate for eradication of SARS-CoV in animal reservoirs, thereby reducing the risk of transmitting the infection to humans.
Url:
DOI: 10.1099/vir.0.80844-0
PubMed: 15831955
PubMed Central: 1361274
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PMC:1361274Le document en format XML
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(génétique)</term><term>DNA-directed RNA polymerases (génétique)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines (génétique)</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Modèles animaux</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines virales (génétique)</term><term>Rhabdoviridae (génétique)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins synthétiques (génétique)</term><term>Vaccins synthétiques (immunologie)</term><term>Vecteurs génétiques</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>DNA-Directed RNA Polymerases</term><term>Glycoproteins</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Vaccines, Synthetic</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Vaccines, Synthetic</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rhabdoviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>DNA-directed RNA polymerases</term><term>Glycoprotéines</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Protéines virales</term><term>Rhabdoviridae</term><term>Vaccins antiviraux</term><term>Vaccins synthétiques</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Vaccins antiviraux</term><term>Vaccins synthétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Genetic Vectors</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Models, Animal</term><term>Neutralization Tests</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Modèles animaux</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Foreign viral proteins expressed by rabies virus (RV) have been shown to induce potent humoral and cellular immune responses in immunized animals. In addition, highly attenuated and, therefore, very safe RV-based vectors have been constructed. Here, an RV-based vaccine vehicle was utilized as a novel vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). For this approach, the SARS-CoV nucleocapsid protein (N) or envelope spike protein (S) genes were cloned between the RV glycoprotein G and polymerase L genes. Recombinant vectors expressing SARS-CoV N or S protein were recovered and their immunogenicity was studied in mice. A single inoculation with the RV-based vaccine expressing SARS-CoV S protein induced a strong SARS-CoV-neutralizing antibody response. The ability of the RV-SARS-CoV S vector to confer immunity after a single inoculation makes this live vaccine a promising candidate for eradication of SARS-CoV in animal reservoirs, thereby reducing the risk of transmitting the infection to humans.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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