An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles.
Identifieur interne : 004C57 ( Main/Merge ); précédent : 004C56; suivant : 004C58An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles.
Auteurs : Parviz Ashtari [République populaire de Chine] ; Xiaoxiao He ; Kemin Wang ; Ping GongSource :
- Talanta [ 1873-3573 ] ; 2005.
Abstract
In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.
DOI: 10.1016/j.talanta.2005.06.043
PubMed: 18970204
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This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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