Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E.
Identifieur interne : 004C06 ( Main/Merge ); précédent : 004C05; suivant : 004C07Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E.
Auteurs : Bone S F. Tang [République populaire de Chine] ; Kwok-Hung Chan ; Vincent C C. Cheng ; Patrick C Y. Woo ; Susanna K P. Lau ; Clarence C K. Lam ; Tsun-Leung Chan ; Alan K L. Wu ; Ivan F N. Hung ; Suet-Yi Leung ; Kwok-Yung YuenSource :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Analyse sur microréseau, Apoptose (génétique), Coronavirus humain 229E (génétique), Coronavirus humain 229E (physiologie), Gènes (génétique), Humains, Infections à coronavirus (génétique), Lignée cellulaire tumorale, RT-PCR, Rhume banal (génétique), Réaction de polymérisation en chaîne, Syndrome respiratoire aigu sévère (génétique), Transcription génétique, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- génétique : ARN messager, Apoptose, Coronavirus humain 229E, Gènes, Infections à coronavirus, Rhume banal, Syndrome respiratoire aigu sévère, Virus du SRAS.
- physiologie : Coronavirus humain 229E, Virus du SRAS.
- Analyse sur microréseau, Humains, Lignée cellulaire tumorale, RT-PCR, Réaction de polymérisation en chaîne, Transcription génétique.
English descriptors
- KwdEn :
- Apoptosis (genetics), Cell Line, Tumor, Common Cold (genetics), Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (physiology), Coronavirus Infections (genetics), Genes (genetics), Humans, Microarray Analysis, Polymerase Chain Reaction, RNA, Messenger (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (physiology), Severe Acute Respiratory Syndrome (genetics), Transcription, Genetic.
- MESH :
- chemical , genetics : RNA, Messenger.
- genetics : Apoptosis, Common Cold, Coronavirus 229E, Human, Coronavirus Infections, Genes, SARS Virus, Severe Acute Respiratory Syndrome.
- physiology : Coronavirus 229E, Human, SARS Virus.
- Cell Line, Tumor, Humans, Microarray Analysis, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic.
Abstract
The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.
DOI: 10.1128/JVI.79.10.6180-6193.2005
PubMed: 15858003
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pubmed:15858003Le document en format XML
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Tang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. 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Tang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Center of Infection and Immunology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. Cheng</name></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name></author><author><name sortKey="Lam, Clarence C K" sort="Lam, Clarence C K" uniqKey="Lam C" first="Clarence C K" last="Lam">Clarence C K. Lam</name></author><author><name sortKey="Chan, Tsun Leung" sort="Chan, Tsun Leung" uniqKey="Chan T" first="Tsun-Leung" last="Chan">Tsun-Leung Chan</name></author><author><name sortKey="Wu, Alan K L" sort="Wu, Alan K L" uniqKey="Wu A" first="Alan K L" last="Wu">Alan K L. Wu</name></author><author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F N" last="Hung">Ivan F N. Hung</name></author><author><name sortKey="Leung, Suet Yi" sort="Leung, Suet Yi" uniqKey="Leung S" first="Suet-Yi" last="Leung">Suet-Yi Leung</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis (genetics)</term><term>Cell Line, Tumor</term><term>Common Cold (genetics)</term><term>Coronavirus 229E, Human (genetics)</term><term>Coronavirus 229E, Human (physiology)</term><term>Coronavirus Infections (genetics)</term><term>Genes (genetics)</term><term>Humans</term><term>Microarray Analysis</term><term>Polymerase Chain Reaction</term><term>RNA, Messenger (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (genetics)</term><term>Transcription, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Analyse sur microréseau</term><term>Apoptose (génétique)</term><term>Coronavirus humain 229E (génétique)</term><term>Coronavirus humain 229E (physiologie)</term><term>Gènes (génétique)</term><term>Humains</term><term>Infections à coronavirus (génétique)</term><term>Lignée cellulaire tumorale</term><term>RT-PCR</term><term>Rhume banal (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Syndrome respiratoire aigu sévère (génétique)</term><term>Transcription génétique</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Apoptosis</term><term>Common Cold</term><term>Coronavirus 229E, Human</term><term>Coronavirus Infections</term><term>Genes</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Apoptose</term><term>Coronavirus humain 229E</term><term>Gènes</term><term>Infections à coronavirus</term><term>Rhume banal</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus humain 229E</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus 229E, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Humans</term><term>Microarray Analysis</term><term>Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur microréseau</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>RT-PCR</term><term>Réaction de polymérisation en chaîne</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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