Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.
Identifieur interne : 004017 ( Main/Merge ); précédent : 004016; suivant : 004018Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.
Auteurs : Ming-Liang He [Hong Kong] ; Bo-Jian Zheng ; Ying Chen ; Kin-Ling Wong ; Jian-Dong Huang ; Marie C. Lin ; Ying Peng ; Kwok Y. Yuen ; Joseph J Y. Sung ; Hsiang-Fu KungSource :
- FEBS letters [ 0014-5793 ] ; 2006.
Descripteurs français
- KwdFr :
- Amorces ADN, Animaux, Cellules cultivées, Cinétique, Effet cytopathogène viral, Macaca mulatta, Petit ARN interférent, Protéines virales (génétique), RT-PCR, Réplication virale, Séquence nucléotidique, Virus du SRAS (enzymologie), Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- enzymologie : Virus du SRAS.
- génétique : Protéines virales, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- Amorces ADN, Animaux, Cellules cultivées, Cinétique, Effet cytopathogène viral, Macaca mulatta, Petit ARN interférent, RT-PCR, Réplication virale, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cells, Cultured, Cytopathogenic Effect, Viral, DNA Primers, Kinetics, Macaca mulatta, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (enzymology), SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Viral Proteins (genetics), Virus Replication.
- MESH :
- chemical , genetics : Viral Proteins.
- chemical : DNA Primers, RNA, Small Interfering.
- enzymology : SARS Virus.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- Animals, Base Sequence, Cells, Cultured, Cytopathogenic Effect, Viral, Kinetics, Macaca mulatta, Reverse Transcriptase Polymerase Chain Reaction, Virus Replication.
Abstract
SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.
DOI: 10.1016/j.febslet.2006.03.066
PubMed: 16638566
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pubmed:16638566Le document en format XML
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Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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