The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein
Identifieur interne : 003C71 ( Main/Merge ); précédent : 003C70; suivant : 003C72The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein
Auteurs : Corrin E. Mcbride [États-Unis] ; JIE LI [États-Unis] ; Carolyn E. Machamer [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2007.
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Abstract
Like other coronaviruses, severe acute respiratory syndrome coronavirus (SARS CoV) assembles at and buds into the lumen of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC). Accumulation of the viral envelope proteins at this compartment is a prerequisite for virus assembly. Previously, we reported the identification of a dibasic motif (KxHxx) in the cytoplasmic tail of the SARS CoV spike (S) protein that was similar to a canonical dilysine ER retrieval signal. Here we demonstrate that this motif is a novel and functional ER retrieval signal which reduced the rate of traffic of the full-length S protein through the Golgi complex. The KxHxx motif also partially retained two different reporter proteins in the ERGIC region and reduced their rates of trafficking, although the motif was less potent than the canonical dilysine signal. The dibasic motif bound the coatomer complex I (COPI) in an in vitro binding assay, suggesting that ER retrieval may contribute to the accumulation of SARS CoV S protein near the virus assembly site for interaction with other viral structural proteins. In support of this, we found that the dibasic motif on the SARS S protein was required for its localization to the ERGIC/Golgi region when coexpressed with SARS membrane (M) protein. Thus, the cycling of SARS S through the ER-Golgi system may be required for its incorporation into assembling virions in the ERGIC.
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Mcbride</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author><author><name sortKey="Jie Li" sort="Jie Li" uniqKey="Jie Li" last="Jie Li">JIE LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author><author><name sortKey="Machamer, Carolyn E" sort="Machamer, Carolyn E" uniqKey="Machamer C" first="Carolyn E." last="Machamer">Carolyn E. Machamer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0143955</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0143955 INIST</idno><idno type="RBID">Pascal:07-0143955</idno><idno type="wicri:Area/PascalFrancis/Corpus">000400</idno><idno type="wicri:Area/PascalFrancis/Curation">000589</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000324</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000324</idno><idno type="wicri:doubleKey">0022-538X:2007:Mcbride C:the:cytoplasmic:tail</idno><idno type="wicri:Area/Main/Merge">003C71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein</title><author><name sortKey="Mcbride, Corrin E" sort="Mcbride, Corrin E" uniqKey="Mcbride C" first="Corrin E." last="Mcbride">Corrin E. Mcbride</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author><author><name sortKey="Jie Li" sort="Jie Li" uniqKey="Jie Li" last="Jie Li">JIE LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author><author><name sortKey="Machamer, Carolyn E" sort="Machamer, Carolyn E" uniqKey="Machamer C" first="Carolyn E." last="Machamer">Carolyn E. Machamer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Cell Biology, The Johns Hopkins University School of Medicine</s1><s2>Baltimore, Maryland 21205</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Baltimore, Maryland 21205</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Endoplasmic reticulum</term><term>Membrane protein</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Protéine membranaire</term><term>Réticulum endoplasmique</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Like other coronaviruses, severe acute respiratory syndrome coronavirus (SARS CoV) assembles at and buds into the lumen of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC). Accumulation of the viral envelope proteins at this compartment is a prerequisite for virus assembly. Previously, we reported the identification of a dibasic motif (KxHxx) in the cytoplasmic tail of the SARS CoV spike (S) protein that was similar to a canonical dilysine ER retrieval signal. Here we demonstrate that this motif is a novel and functional ER retrieval signal which reduced the rate of traffic of the full-length S protein through the Golgi complex. The KxHxx motif also partially retained two different reporter proteins in the ERGIC region and reduced their rates of trafficking, although the motif was less potent than the canonical dilysine signal. The dibasic motif bound the coatomer complex I (COPI) in an in vitro binding assay, suggesting that ER retrieval may contribute to the accumulation of SARS CoV S protein near the virus assembly site for interaction with other viral structural proteins. In support of this, we found that the dibasic motif on the SARS S protein was required for its localization to the ERGIC/Golgi region when coexpressed with SARS membrane (M) protein. Thus, the cycling of SARS S through the ER-Golgi system may be required for its incorporation into assembling virions in the ERGIC.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Mcbride, Corrin E" sort="Mcbride, Corrin E" uniqKey="Mcbride C" first="Corrin E." last="Mcbride">Corrin E. Mcbride</name></noRegion><name sortKey="Jie Li" sort="Jie Li" uniqKey="Jie Li" last="Jie Li">JIE LI</name><name sortKey="Machamer, Carolyn E" sort="Machamer, Carolyn E" uniqKey="Machamer C" first="Carolyn E." last="Machamer">Carolyn E. Machamer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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