SrasV1, Main, Merge, bibRecord, 003925

Amino Acid Substitutions in the S2 Region Enhance Severe Acute Respiratory Syndrome Coronavirus Infectivity in Rat Angiotensin-Converting Enzyme 2-Expressing Cells▿

Identifieur interne : 003925 ( Main/Merge ); précédent : 003924; suivant : 003926

Amino Acid Substitutions in the S2 Region Enhance Severe Acute Respiratory Syndrome Coronavirus Infectivity in Rat Angiotensin-Converting Enzyme 2-Expressing Cells▿

Auteurs : Shuetsu Fukushi ; Tetsuya Mizutani ; Kouji Sakai ; Masayuki Saijo ; Fumihiro Taguchi ; Masaru Yokoyama ; Ichiro Kurane ; Shigeru Morikawa

Source :

Journal of Virology [ 0022-538X ] ; 2007.

RBID : PMC:2045470

Descripteurs français

KwdFr :
- Animaux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Humains, Lignée cellulaire, Peptidyl-Dipeptidase A (métabolisme), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Rats, Réplication virale (génétique), Substitution d'acide aminé, Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Réplication virale, Virus du SRAS.
- métabolisme : Peptidyl-Dipeptidase A.
- physiologie : Virus du SRAS.
- Animaux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Protéines de l'enveloppe virale, Rats, Substitution d'acide aminé.

English descriptors

KwdEn :
- Amino Acid Substitution, Animals, Cell Line, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Peptidyl-Dipeptidase A (metabolism), Rats, SARS Virus (genetics), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Virus Replication (genetics).
MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Peptidyl-Dipeptidase A.
- genetics : SARS Virus, Virus Replication.
- physiology : SARS Virus.
- Amino Acid Substitution, Animals, Cell Line, Humans, Rats, Spike Glycoprotein, Coronavirus.

Abstract

To clarify the molecular basis of severe acute respiratory syndrome coronavirus (SARS-CoV) adaptation to different host species, we serially passaged SARS-CoV in rat angiotensin-converting enzyme 2 (ACE2)-expressing cells. After 15 passages, the virus (Rat-P15) came to replicate effectively in rat ACE2-expressing cells. Two amino acid substitutions in the S2 region were found on the Rat-P15 S gene. Analyses of the infectivity of the pseudotype-bearing S protein indicated that the two substitutions in the S2 region, especially the S950F substitution, were responsible for efficient infection. Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2045470

DOI: 10.1128/JVI.01143-07
PubMed: 17652383
PubMed Central: 2045470

Links to Exploration step

PMC:2045470

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<front><div type="abstract" xml:lang="en"><p>To clarify the molecular basis of severe acute respiratory syndrome coronavirus (SARS-CoV) adaptation to different host species, we serially passaged SARS-CoV in rat angiotensin-converting enzyme 2 (ACE2)-expressing cells. After 15 passages, the virus (Rat-P15) came to replicate effectively in rat ACE2-expressing cells. Two amino acid substitutions in the S2 region were found on the Rat-P15 S gene. Analyses of the infectivity of the pseudotype-bearing S protein indicated that the two substitutions in the S2 region, especially the S950F substitution, were responsible for efficient infection. Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region.</p>
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Amino Acid Substitutions in the S2 Region Enhance Severe Acute Respiratory Syndrome Coronavirus Infectivity in Rat Angiotensin-Converting Enzyme 2-Expressing Cells▿

Amino Acid Substitutions in the S2 Region Enhance Severe Acute Respiratory Syndrome Coronavirus Infectivity in Rat Angiotensin-Converting Enzyme 2-Expressing Cells▿

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

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Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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