Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: implications for a subunit vaccine.
Identifieur interne : 003820 ( Main/Merge ); précédent : 003819; suivant : 003821Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: implications for a subunit vaccine.
Auteurs : Alexander N. Zakhartchouk [Canada] ; Chetna Sharon ; Malathy Satkunarajah ; Thierry Auperin ; Sathiyanarayanan Viswanathan ; George Mutwiri ; Martin Petric ; Raymond H. See ; Robert C. Brunham ; B Brett Finlay ; Cheryl Cameron ; David J. Kelvin ; Alan Cochrane ; James M. Rini ; Lorne A. BabiukSource :
- Vaccine [ 0264-410X ] ; 2007.
Descripteurs français
- KwdFr :
- Alun, Animaux, Anticorps antiviraux (sang), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Interféron gamma (biosynthèse), Lignée cellulaire, Oligodésoxyribonucléotides, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Souris, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux.
- biosynthèse : Interféron gamma.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux.
- sang : Anticorps antiviraux.
- Alun, Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Oligodésoxyribonucléotides, Protéines de l'enveloppe virale, Souris, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Alum Compounds, Animals, Antibodies, Viral (blood), Cell Line, Female, Humans, Interferon-gamma (biosynthesis), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Oligodeoxyribonucleotides, Receptors, Virus (metabolism), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , biosynthesis : Interferon-gamma.
- chemical , blood : Antibodies, Viral.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , metabolism : Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemical : Alum Compounds, Oligodeoxyribonucleotides, Spike Glycoprotein, Coronavirus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Female, Humans, Mice.
Abstract
We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-gamma-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-gamma-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.
DOI: 10.1016/j.vaccine.2006.06.084
PubMed: 16919855
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pubmed:16919855Le document en format XML
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Zakhartchouk</name><affiliation wicri:level="1"><nlm:affiliation>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask., Canada S7N 5E3. zakhartchouk@sask.usask.ca</nlm:affiliation><country>Canada</country><wicri:regionArea>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask.</wicri:regionArea><wicri:noRegion>Sask.</wicri:noRegion></affiliation></author><author><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name></author><author><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name></author><author><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name></author><author><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name></author><author><name sortKey="Mutwiri, George" sort="Mutwiri, George" uniqKey="Mutwiri G" first="George" last="Mutwiri">George Mutwiri</name></author><author><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name></author><author><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H" last="See">Raymond H. 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Zakhartchouk</name><affiliation wicri:level="1"><nlm:affiliation>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask., Canada S7N 5E3. zakhartchouk@sask.usask.ca</nlm:affiliation><country>Canada</country><wicri:regionArea>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask.</wicri:regionArea><wicri:noRegion>Sask.</wicri:noRegion></affiliation></author><author><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name></author><author><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name></author><author><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name></author><author><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name></author><author><name sortKey="Mutwiri, George" sort="Mutwiri, George" uniqKey="Mutwiri G" first="George" last="Mutwiri">George Mutwiri</name></author><author><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name></author><author><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H" last="See">Raymond H. 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(immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alun</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Interféron gamma (biosynthèse)</term><term>Lignée cellulaire</term><term>Oligodésoxyribonucléotides</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Souris</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral 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viraux</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Female</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alun</term><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Oligodésoxyribonucléotides</term><term>Protéines de l'enveloppe virale</term><term>Souris</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-gamma-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-gamma-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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