[Establishment of a system for determination of emerging infectious diseases (RDV method), and its application].
Identifieur interne : 003651 ( Main/Merge ); précédent : 003650; suivant : 003652[Establishment of a system for determination of emerging infectious diseases (RDV method), and its application].
Auteurs : Tetsuya Mizutani [Japon]Source :
- Uirusu [ 0042-6857 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ARN (), Animaux, Banque de gènes, Culicidae (virologie), Flambées de maladies (), Humains, Maladies transmissibles émergentes (), Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (virologie), Maladies virales (), Maladies virales (diagnostic), Maladies virales (virologie), Techniques d'amplification d'acides nucléiques (), Virus (génétique), Virus (isolement et purification).
- MESH :
- diagnostic : Maladies transmissibles émergentes, Maladies virales.
- génétique : ARN viral, Virus.
- isolement et purification : Virus.
- virologie : Culicidae, Maladies transmissibles émergentes, Maladies virales.
- Analyse de séquence d'ARN, Animaux, Banque de gènes, Flambées de maladies, Humains, Maladies transmissibles émergentes, Maladies virales, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- Animals, Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (prevention & control), Communicable Diseases, Emerging (virology), Culicidae (virology), Disease Outbreaks (prevention & control), Gene Library, Humans, Nucleic Acid Amplification Techniques (methods), RNA, Viral (genetics), Sequence Analysis, RNA (methods), Virus Diseases (diagnosis), Virus Diseases (prevention & control), Virus Diseases (virology), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- chemical , genetics : RNA, Viral.
- diagnosis : Communicable Diseases, Emerging, Virus Diseases.
- genetics : Viruses.
- isolation & purification : Viruses.
- methods : Nucleic Acid Amplification Techniques, Sequence Analysis, RNA.
- prevention & control : Communicable Diseases, Emerging, Disease Outbreaks, Virus Diseases.
- virology : Communicable Diseases, Emerging, Culicidae, Virus Diseases.
- Animals, Gene Library, Humans.
Abstract
We developed a new system for detection of viral nucleic acids because rapid detection of pathogens is necessary to prevent potential outbreaks of infectious diseases. This system, named rapid determination of viral RNA sequences (RDV), involves whole-genome amplification and a new direct sequencing technique. Using the RDV system, it is possible to obtain viral nucleic acid sequences within 2 days. The nucleic acid sequences of SARS-CoV and West Nile virus, which represent emerging and re-emerging infectious viruses, were determined from infectious viral culture supernatant by the RDV method. We demonstrated the clinical application of this system in human specimens and its diagnostic usage in mosquitoes. Furthermore, new adenovirus and herpesvirus were found in bats using the RDV method.
DOI: 10.2222/jsv.57.217
PubMed: 18357760
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pubmed:18357760Le document en format XML
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<term>Culicidae (virology)</term>
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<term>Gene Library</term>
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<term>Maladies virales (virologie)</term>
<term>Techniques d'amplification d'acides nucléiques ()</term>
<term>Virus (génétique)</term>
<term>Virus (isolement et purification)</term>
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<term>Maladies virales</term>
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<term>Maladies virales</term>
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<term>Banque de gènes</term>
<term>Flambées de maladies</term>
<term>Humains</term>
<term>Maladies transmissibles émergentes</term>
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<front><div type="abstract" xml:lang="en">We developed a new system for detection of viral nucleic acids because rapid detection of pathogens is necessary to prevent potential outbreaks of infectious diseases. This system, named rapid determination of viral RNA sequences (RDV), involves whole-genome amplification and a new direct sequencing technique. Using the RDV system, it is possible to obtain viral nucleic acid sequences within 2 days. The nucleic acid sequences of SARS-CoV and West Nile virus, which represent emerging and re-emerging infectious viruses, were determined from infectious viral culture supernatant by the RDV method. We demonstrated the clinical application of this system in human specimens and its diagnostic usage in mosquitoes. Furthermore, new adenovirus and herpesvirus were found in bats using the RDV method.</div>
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