Evaluation of cotton rats as a model for severe acute respiratory syndrome.
Identifieur interne : 003203 ( Main/Merge ); précédent : 003202; suivant : 003204Evaluation of cotton rats as a model for severe acute respiratory syndrome.
Auteurs : D M Watts [États-Unis] ; C J Peters ; P. Newman ; N. Wang ; N. Yoshikawa ; C K Tseng ; P R WydeSource :
- Vector borne and zoonotic diseases (Larchmont, N.Y.) [ 1530-3667 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Syndrome respiratoire aigu sévère.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Femelle, Modèles animaux de maladie humaine, Mâle, Projets pilotes, Réplication virale, Sigmodontinae.
English descriptors
- KwdEn :
- MESH :
- pathology : Severe Acute Respiratory Syndrome.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Female, Male, Pilot Projects, Sigmodontinae, Vero Cells, Virus Replication.
Abstract
Experimental studies were conducted to evaluate two species of cotton rats, Sigmodon hispidus and Sigmodon fulviventer, as a model for severe acute respiratory syndrome (SARS). Blood and turbinate wash samples, and lung tissue were collected from each animal at different time points after SARS coronavirus (CoV) infection for determining the growth curve of virus, if any, by the standard infectivity assay in Vero E6 cells. In addition, sections of the lung, liver, spleen, and kidney were taken and used for histology analysis. All animals were observed daily for signs of illness, and in some experiments, animals were weighed on the day when they were sacrificed. The results indicated that the cotton rat species, S. hispidus and S. fulviventer, were not a useful model for either SARS-CoV infection or disease. This observation was supported by the absence of any signs of illness, the failure to consistently demonstrate virus in the blood and tissues, and the absent of any notable histopathology. However, infected animals were capable of producing neutralizing antibodies against SARS-CoV, suggesting the seroconversion did occur. Further studies are warranted to consider other animal species in efforts to find better animal models for the evaluation of SARS-CoV vaccines and antiviral drugs.
DOI: 10.1089/vbz.2007.0210
PubMed: 18447621
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001B59
- to stream PubMed, to step Curation: 001B59
- to stream PubMed, to step Checkpoint: 001B35
- to stream Ncbi, to step Merge: 001C37
- to stream Ncbi, to step Curation: 001C37
- to stream Ncbi, to step Checkpoint: 001C37
Links to Exploration step
pubmed:18447621Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of cotton rats as a model for severe acute respiratory syndrome.</title>
<author><name sortKey="Watts, D M" sort="Watts, D M" uniqKey="Watts D" first="D M" last="Watts">D M Watts</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. dowatts@utmb.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name>
</author>
<author><name sortKey="Newman, P" sort="Newman, P" uniqKey="Newman P" first="P" last="Newman">P. Newman</name>
</author>
<author><name sortKey="Wang, N" sort="Wang, N" uniqKey="Wang N" first="N" last="Wang">N. Wang</name>
</author>
<author><name sortKey="Yoshikawa, N" sort="Yoshikawa, N" uniqKey="Yoshikawa N" first="N" last="Yoshikawa">N. Yoshikawa</name>
</author>
<author><name sortKey="Tseng, C K" sort="Tseng, C K" uniqKey="Tseng C" first="C K" last="Tseng">C K Tseng</name>
</author>
<author><name sortKey="Wyde, P R" sort="Wyde, P R" uniqKey="Wyde P" first="P R" last="Wyde">P R Wyde</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2008">2008</date>
<idno type="RBID">pubmed:18447621</idno>
<idno type="pmid">18447621</idno>
<idno type="doi">10.1089/vbz.2007.0210</idno>
<idno type="wicri:Area/PubMed/Corpus">001B59</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001B59</idno>
<idno type="wicri:Area/PubMed/Curation">001B59</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001B59</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001B35</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001B35</idno>
<idno type="wicri:Area/Ncbi/Merge">001C37</idno>
<idno type="wicri:Area/Ncbi/Curation">001C37</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001C37</idno>
<idno type="wicri:doubleKey">1530-3667:2008:Watts D:evaluation:of:cotton</idno>
<idno type="wicri:Area/Main/Merge">003203</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of cotton rats as a model for severe acute respiratory syndrome.</title>
<author><name sortKey="Watts, D M" sort="Watts, D M" uniqKey="Watts D" first="D M" last="Watts">D M Watts</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. dowatts@utmb.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name>
</author>
<author><name sortKey="Newman, P" sort="Newman, P" uniqKey="Newman P" first="P" last="Newman">P. Newman</name>
</author>
<author><name sortKey="Wang, N" sort="Wang, N" uniqKey="Wang N" first="N" last="Wang">N. Wang</name>
</author>
<author><name sortKey="Yoshikawa, N" sort="Yoshikawa, N" uniqKey="Yoshikawa N" first="N" last="Yoshikawa">N. Yoshikawa</name>
</author>
<author><name sortKey="Tseng, C K" sort="Tseng, C K" uniqKey="Tseng C" first="C K" last="Tseng">C K Tseng</name>
</author>
<author><name sortKey="Wyde, P R" sort="Wyde, P R" uniqKey="Wyde P" first="P R" last="Wyde">P R Wyde</name>
</author>
</analytic>
<series><title level="j">Vector borne and zoonotic diseases (Larchmont, N.Y.)</title>
<idno type="ISSN">1530-3667</idno>
<imprint><date when="2008" type="published">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Disease Models, Animal</term>
<term>Female</term>
<term>Male</term>
<term>Pilot Projects</term>
<term>SARS Virus (physiology)</term>
<term>Severe Acute Respiratory Syndrome (pathology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Sigmodontinae</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Femelle</term>
<term>Modèles animaux de maladie humaine</term>
<term>Mâle</term>
<term>Projets pilotes</term>
<term>Réplication virale</term>
<term>Sigmodontinae</term>
<term>Syndrome respiratoire aigu sévère (anatomopathologie)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Disease Models, Animal</term>
<term>Female</term>
<term>Male</term>
<term>Pilot Projects</term>
<term>Sigmodontinae</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Femelle</term>
<term>Modèles animaux de maladie humaine</term>
<term>Mâle</term>
<term>Projets pilotes</term>
<term>Réplication virale</term>
<term>Sigmodontinae</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Experimental studies were conducted to evaluate two species of cotton rats, Sigmodon hispidus and Sigmodon fulviventer, as a model for severe acute respiratory syndrome (SARS). Blood and turbinate wash samples, and lung tissue were collected from each animal at different time points after SARS coronavirus (CoV) infection for determining the growth curve of virus, if any, by the standard infectivity assay in Vero E6 cells. In addition, sections of the lung, liver, spleen, and kidney were taken and used for histology analysis. All animals were observed daily for signs of illness, and in some experiments, animals were weighed on the day when they were sacrificed. The results indicated that the cotton rat species, S. hispidus and S. fulviventer, were not a useful model for either SARS-CoV infection or disease. This observation was supported by the absence of any signs of illness, the failure to consistently demonstrate virus in the blood and tissues, and the absent of any notable histopathology. However, infected animals were capable of producing neutralizing antibodies against SARS-CoV, suggesting the seroconversion did occur. Further studies are warranted to consider other animal species in efforts to find better animal models for the evaluation of SARS-CoV vaccines and antiviral drugs.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003203 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003203 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:18447621 |texte= Evaluation of cotton rats as a model for severe acute respiratory syndrome. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:18447621" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |