Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus.
Identifieur interne : 003170 ( Main/Merge ); précédent : 003169; suivant : 003171Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus.
Auteurs : Yi-Ching Hsieh [Taïwan] ; Hui-Chun Li ; Shih-Chi Chen ; Shih-Yen LoSource :
- Journal of biomedical science [ 1423-0127 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Cartographie d'interactions entre protéines, Cellules Vero, Délétion de gène, Liaison aux protéines, Protéines de la matrice virale (métabolisme), Protéines virales structurales (métabolisme), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Virus du SRAS.
- métabolisme : Protéines de la matrice virale, Protéines virales structurales, Syndrome respiratoire aigu sévère, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cartographie d'interactions entre protéines, Cellules Vero, Délétion de gène, Liaison aux protéines.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Gene Deletion, Protein Binding, Protein Interaction Mapping, SARS Virus (genetics), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Matrix Proteins (metabolism), Viral Structural Proteins (metabolism).
- MESH :
- chemical , metabolism : Viral Matrix Proteins, Viral Structural Proteins.
- genetics : SARS Virus.
- metabolism : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Gene Deletion, Protein Binding, Protein Interaction Mapping, Vero Cells.
Abstract
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is co-localized almost entirely with S, E, or NC protein when co-expressed in the cells. On the other hand, only partial co-localization was observed when S and E, S and NC, or E and NC were co-expressed in the cells. Interactions between SARS-CoV M and other structural proteins but not interactions between S and E, S and NC, or E and NC were further demonstrated by co-immunoprecipitation assay. These results indicate that SARS-CoV M protein, similar to the M proteins of other coronaviruses, plays a pivotal role in virus assembly. The cytoplasmic C-terminus domain of SARS-CoV M protein was responsible for binding to NC protein. Multiple regions of M protein interacted with E and S proteins. A model for the interactions between SARS-CoV M protein and other structural proteins is proposed. This study helps us better understand protein-protein interactions during viral assembly of SARS-CoV.
DOI: 10.1007/s11373-008-9278-3
PubMed: 18792806
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pubmed:18792806Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is co-localized almost entirely with S, E, or NC protein when co-expressed in the cells. On the other hand, only partial co-localization was observed when S and E, S and NC, or E and NC were co-expressed in the cells. Interactions between SARS-CoV M and other structural proteins but not interactions between S and E, S and NC, or E and NC were further demonstrated by co-immunoprecipitation assay. These results indicate that SARS-CoV M protein, similar to the M proteins of other coronaviruses, plays a pivotal role in virus assembly. The cytoplasmic C-terminus domain of SARS-CoV M protein was responsible for binding to NC protein. Multiple regions of M protein interacted with E and S proteins. A model for the interactions between SARS-CoV M protein and other structural proteins is proposed. This study helps us better understand protein-protein interactions during viral assembly of SARS-CoV.</div>
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