SrasV1, Main, Exploration, bibRecordById, pubmed:8381459

Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for fusion activity.

Identifieur interne : 006583 ( Main/Exploration ); précédent : 006582; suivant : 006584

Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for fusion activity.

Auteurs : R. Stauber [Allemagne] ; M. Pfleiderera ; S. Siddell

Source :

The Journal of general virology [ 0022-1317 ] ; 1993.

RBID : pubmed:8381459

Descripteurs français

KwdFr :
- Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Glycoprotéines membranaires (physiologie), Protéines de fusion virale (métabolisme), Protéines de l'enveloppe virale, Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines de la matrice virale (physiologie), Séquence d'acides aminés, Séquence nucléotidique, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (métabolisme).
MESH :
- génétique : Glycoprotéines membranaires, Protéines de la matrice virale, Virus de l'hépatite murine.
- métabolisme : Glycoprotéines membranaires, Protéines de fusion virale, Protéines de la matrice virale, Virus de l'hépatite murine.
- physiologie : Glycoprotéines membranaires, Protéines de la matrice virale.
- Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Protéines de l'enveloppe virale, Séquence d'acides aminés, Séquence nucléotidique.

English descriptors

KwdEn :
- Amino Acid Sequence, Base Sequence, Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Membrane Glycoproteins (physiology), Molecular Sequence Data, Murine hepatitis virus (genetics), Murine hepatitis virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Viral Fusion Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Viral Matrix Proteins (physiology).
MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Matrix Proteins.
- chemical , metabolism : Membrane Glycoproteins, Viral Fusion Proteins, Viral Matrix Proteins.
- chemical , physiology : Membrane Glycoproteins, Viral Matrix Proteins.
- genetics : Murine hepatitis virus.
- metabolism : Murine hepatitis virus.
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins.

Abstract

A cDNA copy of the murine coronavirus [otherwise known as murine hepatitis virus (MHV)] surface (S) glycoprotein gene was isolated and expressed in DBT cells by using a recombinant vaccinia virus system. The expressed S protein induced extensive syncytium formation at neutral pH. Oligonucleotide mutagenesis was used to engineer an S protein gene in which codons for the proteolytic cleavage site, Arg-Arg-Ala-Arg-Arg, were replaced with an equal number of codons for amino acids with aliphatic or aliphatic hydroxyl side-chains. The mutated S protein was stably expressed in DBT cells and, in contrast to the wild-type protein, was not proteolytically cleaved. Nevertheless, the non-cleaved protein induced extensive syncytium formation. These results clearly indicate that the non-cleaved form of the MHV S protein is able to mediate cell membrane fusion. Thus proteolytic cleavage is not an absolute requirement for fusion activity.

DOI: 10.1099/0022-1317-74-2-183
PubMed: 8381459

Affiliations:

Allemagne

Le document en format XML

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<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Protéines de fusion virale (métabolisme)</term>
<term>Protéines de l'enveloppe virale</term>
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<term>Protéines de la matrice virale (métabolisme)</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">A cDNA copy of the murine coronavirus [otherwise known as murine hepatitis virus (MHV)] surface (S) glycoprotein gene was isolated and expressed in DBT cells by using a recombinant vaccinia virus system. The expressed S protein induced extensive syncytium formation at neutral pH. Oligonucleotide mutagenesis was used to engineer an S protein gene in which codons for the proteolytic cleavage site, Arg-Arg-Ala-Arg-Arg, were replaced with an equal number of codons for amino acids with aliphatic or aliphatic hydroxyl side-chains. The mutated S protein was stably expressed in DBT cells and, in contrast to the wild-type protein, was not proteolytically cleaved. Nevertheless, the non-cleaved protein induced extensive syncytium formation. These results clearly indicate that the non-cleaved form of the MHV S protein is able to mediate cell membrane fusion. Thus proteolytic cleavage is not an absolute requirement for fusion activity.</div>
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Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for fusion activity.

Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for fusion activity.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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