Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge.
Identifieur interne : 001994 ( Main/Exploration ); précédent : 001993; suivant : 001995Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge.
Auteurs : Carmina Verdiá-Báguena [Espagne] ; Jose L. Nieto-Torres ; Antonio Alcaraz ; Marta L. Dediego ; Luis Enjuanes ; Vicente M. AguilellaSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2013.
Descripteurs français
- KwdFr :
- Canaux ioniques (), Concentration en ions d'hydrogène, Données de séquences moléculaires, Double couche lipidique (), Modèles moléculaires, Phosphatidylcholines (), Phosphatidylsérine (), Potassium (), Potentiels de membrane, Protéines de l'enveloppe virale (), Structure tertiaire des protéines, Séquence d'acides aminés, Transport des ions, Virus du SRAS (), Électricité statique.
- MESH :
- Canaux ioniques, Concentration en ions d'hydrogène, Données de séquences moléculaires, Double couche lipidique, Modèles moléculaires, Phosphatidylcholines, Phosphatidylsérine, Potassium, Potentiels de membrane, Protéines de l'enveloppe virale, Structure tertiaire des protéines, Séquence d'acides aminés, Transport des ions, Virus du SRAS, Électricité statique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Hydrogen-Ion Concentration, Ion Channels (chemistry), Ion Transport, Lipid Bilayers (chemistry), Membrane Potentials, Models, Molecular, Molecular Sequence Data, Phosphatidylcholines (chemistry), Phosphatidylserines (chemistry), Potassium (chemistry), Protein Structure, Tertiary, SARS Virus (chemistry), Static Electricity, Viral Envelope Proteins (chemistry).
- MESH :
- chemical , chemistry : Ion Channels, Lipid Bilayers, Phosphatidylcholines, Phosphatidylserines, Potassium, Viral Envelope Proteins.
- chemistry : SARS Virus.
- Amino Acid Sequence, Hydrogen-Ion Concentration, Ion Transport, Membrane Potentials, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Static Electricity.
Abstract
A partial characterization of the ion channels formed by the SARS coronavirus (CoV) envelope (E) protein was previously reported (C. Verdiá-Báguena et al., 2012 [12]). Here, we provide new significant insights on the involvement of lipids in the structure and function of the CoV E protein channel on the basis of three series of experiments. First, reversal potential measurements over a wide range of pH allow the dissection of the contributions to channel selectivity coming from ionizable residues of the protein transmembrane domain and also from the negatively charged groups of diphytanoyl phosphatidylserine (DPhPS) lipid. The corresponding effective pKas are consistent with the model pKas of the acidic residue candidates for titration. Second, the change of channel conductance with salt concentration reveals two distinct regimes (Donnan-controlled electrodiffusion and bulk-like electrodiffusion) fully compatible with the outcomes of selectivity experiments. Third, by measuring channel conductance in mixtures of neutral diphytanoyl phosphatidylcholine (DPhPC) lipids and negatively charged DPhPS lipids in low and high salt concentrations we conclude that the protein-lipid conformation in the channel is likely the same in charged and neutral lipids. Overall, the whole set of experiments supports the proteolipidic structure of SARS-CoV E channels and explains the large difference in channel conductance observed between neutral and charged membranes.
DOI: 10.1016/j.bbamem.2013.05.008
PubMed: 23688394
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001195
- to stream PubMed, to step Curation: 001195
- to stream PubMed, to step Checkpoint: 001232
- to stream Ncbi, to step Merge: 002677
- to stream Ncbi, to step Curation: 002677
- to stream Ncbi, to step Checkpoint: 002677
- to stream Main, to step Merge: 001A04
- to stream Main, to step Curation: 001994
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge.</title>
<author><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdiá-Báguena">Carmina Verdiá-Báguena</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nieto Torres, Jose L" sort="Nieto Torres, Jose L" uniqKey="Nieto Torres J" first="Jose L" last="Nieto-Torres">Jose L. Nieto-Torres</name>
</author>
<author><name sortKey="Alcaraz, Antonio" sort="Alcaraz, Antonio" uniqKey="Alcaraz A" first="Antonio" last="Alcaraz">Antonio Alcaraz</name>
</author>
<author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
</author>
<author><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M" last="Aguilella">Vicente M. Aguilella</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23688394</idno>
<idno type="pmid">23688394</idno>
<idno type="doi">10.1016/j.bbamem.2013.05.008</idno>
<idno type="wicri:Area/PubMed/Corpus">001195</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001195</idno>
<idno type="wicri:Area/PubMed/Curation">001195</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001195</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001232</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001232</idno>
<idno type="wicri:Area/Ncbi/Merge">002677</idno>
<idno type="wicri:Area/Ncbi/Curation">002677</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002677</idno>
<idno type="wicri:doubleKey">0006-3002:2013:Verdia Baguena C:analysis:of:sars</idno>
<idno type="wicri:Area/Main/Merge">001A04</idno>
<idno type="wicri:Area/Main/Curation">001994</idno>
<idno type="wicri:Area/Main/Exploration">001994</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge.</title>
<author><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdiá-Báguena">Carmina Verdiá-Báguena</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nieto Torres, Jose L" sort="Nieto Torres, Jose L" uniqKey="Nieto Torres J" first="Jose L" last="Nieto-Torres">Jose L. Nieto-Torres</name>
</author>
<author><name sortKey="Alcaraz, Antonio" sort="Alcaraz, Antonio" uniqKey="Alcaraz A" first="Antonio" last="Alcaraz">Antonio Alcaraz</name>
</author>
<author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
</author>
<author><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M" last="Aguilella">Vicente M. Aguilella</name>
</author>
</analytic>
<series><title level="j">Biochimica et biophysica acta</title>
<idno type="ISSN">0006-3002</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Hydrogen-Ion Concentration</term>
<term>Ion Channels (chemistry)</term>
<term>Ion Transport</term>
<term>Lipid Bilayers (chemistry)</term>
<term>Membrane Potentials</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phosphatidylcholines (chemistry)</term>
<term>Phosphatidylserines (chemistry)</term>
<term>Potassium (chemistry)</term>
<term>Protein Structure, Tertiary</term>
<term>SARS Virus (chemistry)</term>
<term>Static Electricity</term>
<term>Viral Envelope Proteins (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Canaux ioniques ()</term>
<term>Concentration en ions d'hydrogène</term>
<term>Données de séquences moléculaires</term>
<term>Double couche lipidique ()</term>
<term>Modèles moléculaires</term>
<term>Phosphatidylcholines ()</term>
<term>Phosphatidylsérine ()</term>
<term>Potassium ()</term>
<term>Potentiels de membrane</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Transport des ions</term>
<term>Virus du SRAS ()</term>
<term>Électricité statique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ion Channels</term>
<term>Lipid Bilayers</term>
<term>Phosphatidylcholines</term>
<term>Phosphatidylserines</term>
<term>Potassium</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Hydrogen-Ion Concentration</term>
<term>Ion Transport</term>
<term>Membrane Potentials</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
<term>Static Electricity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Canaux ioniques</term>
<term>Concentration en ions d'hydrogène</term>
<term>Données de séquences moléculaires</term>
<term>Double couche lipidique</term>
<term>Modèles moléculaires</term>
<term>Phosphatidylcholines</term>
<term>Phosphatidylsérine</term>
<term>Potassium</term>
<term>Potentiels de membrane</term>
<term>Protéines de l'enveloppe virale</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Transport des ions</term>
<term>Virus du SRAS</term>
<term>Électricité statique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A partial characterization of the ion channels formed by the SARS coronavirus (CoV) envelope (E) protein was previously reported (C. Verdiá-Báguena et al., 2012 [12]). Here, we provide new significant insights on the involvement of lipids in the structure and function of the CoV E protein channel on the basis of three series of experiments. First, reversal potential measurements over a wide range of pH allow the dissection of the contributions to channel selectivity coming from ionizable residues of the protein transmembrane domain and also from the negatively charged groups of diphytanoyl phosphatidylserine (DPhPS) lipid. The corresponding effective pKas are consistent with the model pKas of the acidic residue candidates for titration. Second, the change of channel conductance with salt concentration reveals two distinct regimes (Donnan-controlled electrodiffusion and bulk-like electrodiffusion) fully compatible with the outcomes of selectivity experiments. Third, by measuring channel conductance in mixtures of neutral diphytanoyl phosphatidylcholine (DPhPC) lipids and negatively charged DPhPS lipids in low and high salt concentrations we conclude that the protein-lipid conformation in the channel is likely the same in charged and neutral lipids. Overall, the whole set of experiments supports the proteolipidic structure of SARS-CoV E channels and explains the large difference in channel conductance observed between neutral and charged membranes. </div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
</country>
<region><li>Communauté valencienne</li>
</region>
</list>
<tree><noCountry><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M" last="Aguilella">Vicente M. Aguilella</name>
<name sortKey="Alcaraz, Antonio" sort="Alcaraz, Antonio" uniqKey="Alcaraz A" first="Antonio" last="Alcaraz">Antonio Alcaraz</name>
<name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
<name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<name sortKey="Nieto Torres, Jose L" sort="Nieto Torres, Jose L" uniqKey="Nieto Torres J" first="Jose L" last="Nieto-Torres">Jose L. Nieto-Torres</name>
</noCountry>
<country name="Espagne"><region name="Communauté valencienne"><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdiá-Báguena">Carmina Verdiá-Báguena</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001994 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001994 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:23688394 |texte= Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23688394" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |