Inhibition of the interaction between the SARS-CoV Spike protein and its cellular receptor by anti-histo-blood group antibodies
Identifieur interne : 003207 ( Main/Exploration ); précédent : 003206; suivant : 003208Inhibition of the interaction between the SARS-CoV Spike protein and its cellular receptor by anti-histo-blood group antibodies
Auteurs : Patrice Guillon [France] ; Monique Clément [France] ; Véronique Sébille [France] ; Jean-Gérard Rivain [France] ; Chih-Fong Chou [Singapour] ; Nathalie Ruvoën-Clouet ; Jacques Le Pendu [France]Source :
- Glycobiology [ 0959-6658 ] ; 2008.
Descripteurs français
- KwdFr :
- Adhérence cellulaire (immunologie), Animaux, Antigènes viraux (immunologie), Autoanticorps (immunologie), Cellules CHO, Cellules Vero, Cricetinae, Cricetulus, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (antagonistes et inhibiteurs), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Protéines de l'enveloppe virale (antagonistes et inhibiteurs), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Système ABO de groupes sanguins (immunologie).
- MESH :
- antagonistes et inhibiteurs : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Adhérence cellulaire, Antigènes viraux, Autoanticorps, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Système ABO de groupes sanguins.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- Animaux, Cellules CHO, Cellules Vero, Cricetinae, Cricetulus, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Humains.
English descriptors
- KwdEn :
- ABO Blood-Group System (immunology), Animals, Antigens, Viral (immunology), Autoantibodies (immunology), CHO Cells, Cell Adhesion (immunology), Chlorocebus aethiops, Cricetinae, Cricetulus, Flow Cytometry, Humans, Membrane Glycoproteins (antagonists & inhibitors), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (antagonists & inhibitors), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : ABO Blood-Group System, Antigens, Viral, Autoantibodies, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : Cell Adhesion.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- Animals, CHO Cells, Chlorocebus aethiops, Cricetinae, Cricetulus, Flow Cytometry, Humans, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly pathogenic emergent virus which replicates in cells that can express ABH histo-blood group antigens. The heavily glycosylated SARS-CoV spike (S) protein binds to angiotensin-converting enzyme 2 which serves as a cellular receptor. Epidemiological analysis of a hospital outbreak in Hong Kong revealed that blood group O was associated with a low risk of infection. In this study, we used a cellular model of adhesion to investigate whether natural antibodies of the ABO system could block the S protein and angiotensin-converting enzyme 2 interaction. To this aim, a C-terminally EGFP-tagged S protein was expressed in chinese hamster ovary cells cotransfected with an α1,2-fucosyltransferase and an A-transferase in order to coexpress the S glycoprotein ectodomain and the A antigen at the cell surface. We observed that the S protein/angiotensin-converting enzyme 2-dependent adhesion of these cells to an angiotensin-converting enzyme 2 expressing cell line was specifically inhibited by either a monoclonal or human natural anti-A antibodies, indicating that these antibodies may block the interaction between the virus and its receptor, thereby providing protection. In order to more fully appreciate the potential effect of the ABO polymorphism on the epidemiology of SARS, we built a mathematical model of the virus transmission dynamics that takes into account the protective effect of ABO natural antibodies. The model indicated that the ABO polymorphism could contribute to substantially reduce the virus transmission, affecting both the number of infected individuals and the kinetics of the epidemic.
Url:
- https://api.istex.fr/ark:/67375/HXZ-R0VWLWDJ-5/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108609
DOI: 10.1093/glycob/cwn093
Affiliations:
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Le document en format XML
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inhibitors)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (antagonists & inhibitors)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhérence cellulaire (immunologie)</term><term>Animaux</term><term>Antigènes viraux (immunologie)</term><term>Autoanticorps (immunologie)</term><term>Cellules CHO</term><term>Cellules Vero</term><term>Cricetinae</term><term>Cricetulus</term><term>Cytométrie en flux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (antagonistes et inhibiteurs)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Protéines de l'enveloppe virale (antagonistes et inhibiteurs)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Système ABO de groupes sanguins (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>ABO Blood-Group System</term><term>Antigens, Viral</term><term>Autoantibodies</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Adhérence cellulaire</term><term>Antigènes viraux</term><term>Autoanticorps</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Système ABO de groupes sanguins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cell Adhesion</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Cricetulus</term><term>Flow Cytometry</term><term>Humans</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cellules Vero</term><term>Cricetinae</term><term>Cricetulus</term><term>Cytométrie en flux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly pathogenic emergent virus which replicates in cells that can express ABH histo-blood group antigens. The heavily glycosylated SARS-CoV spike (S) protein binds to angiotensin-converting enzyme 2 which serves as a cellular receptor. Epidemiological analysis of a hospital outbreak in Hong Kong revealed that blood group O was associated with a low risk of infection. In this study, we used a cellular model of adhesion to investigate whether natural antibodies of the ABO system could block the S protein and angiotensin-converting enzyme 2 interaction. To this aim, a C-terminally EGFP-tagged S protein was expressed in chinese hamster ovary cells cotransfected with an α1,2-fucosyltransferase and an A-transferase in order to coexpress the S glycoprotein ectodomain and the A antigen at the cell surface. We observed that the S protein/angiotensin-converting enzyme 2-dependent adhesion of these cells to an angiotensin-converting enzyme 2 expressing cell line was specifically inhibited by either a monoclonal or human natural anti-A antibodies, indicating that these antibodies may block the interaction between the virus and its receptor, thereby providing protection. In order to more fully appreciate the potential effect of the ABO polymorphism on the epidemiology of SARS, we built a mathematical model of the virus transmission dynamics that takes into account the protective effect of ABO natural antibodies. The model indicated that the ABO polymorphism could contribute to substantially reduce the virus transmission, affecting both the number of infected individuals and the kinetics of the epidemic.</div></front></TEI><affiliations><list><country><li>France</li><li>Singapour</li></country><region><li>Pays de la Loire</li></region><settlement><li>Nantes</li></settlement></list><tree><noCountry><name sortKey="Ruvoen Clouet, Nathalie" sort="Ruvoen Clouet, Nathalie" uniqKey="Ruvoen Clouet N" first="Nathalie" last="Ruvoën-Clouet">Nathalie Ruvoën-Clouet</name></noCountry><country name="France"><region name="Pays de la Loire"><name sortKey="Guillon, Patrice" sort="Guillon, Patrice" uniqKey="Guillon P" first="Patrice" last="Guillon">Patrice Guillon</name></region><name sortKey="Clement, Monique" sort="Clement, Monique" uniqKey="Clement M" first="Monique" last="Clément">Monique Clément</name><name sortKey="Le Pendu, Jacques" sort="Le Pendu, Jacques" uniqKey="Le Pendu J" first="Jacques" last="Le Pendu">Jacques Le Pendu</name><name sortKey="Le Pendu, Jacques" sort="Le Pendu, Jacques" uniqKey="Le Pendu J" first="Jacques" last="Le Pendu">Jacques Le Pendu</name><name sortKey="Le Pendu, Jacques" sort="Le Pendu, Jacques" uniqKey="Le Pendu J" first="Jacques" last="Le Pendu">Jacques Le Pendu</name><name sortKey="Rivain, Jean Gerard" sort="Rivain, Jean Gerard" uniqKey="Rivain J" first="Jean-Gérard" last="Rivain">Jean-Gérard Rivain</name><name sortKey="Sebille, Veronique" sort="Sebille, Veronique" uniqKey="Sebille V" first="Véronique" last="Sébille">Véronique Sébille</name></country><country name="Singapour"><noRegion><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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