siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Identifieur interne : 003244 ( Main/Exploration ); précédent : 003243; suivant : 003245siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Auteurs : C.-Y. Lu [Taïwan] ; H.-Y. Huang [Taïwan] ; T.-H. Yang [Taïwan] ; L.-Y. Chang [Taïwan] ; C.-Y. Lee [Taïwan] ; L.-M. Huang [Taïwan]Source :
- European Journal of Clinical Microbiology & Infectious Diseases [ 0934-9723 ] ; 2008-08-01.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Peptidyl-Dipeptidase A.
- métabolisme : Peptidyl-Dipeptidase A.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- Animaux, Cellules Vero, Chats, Extinction de l'expression des gènes, Lignée cellulaire, Réplication virale, Virus du SRAS.
- Pascal (Inist)
English descriptors
- KwdEn :
- Angiotensin, Animals, Cats, Cell Line, Chlorocebus aethiops, Coronavirus, Gene Silencing (drug effects), Microbiology, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), RNA, Small Interfering (pharmacology), Replication, SARS Virus (drug effects), SARS Virus (physiology), Severe acute respiratory syndrome, Vero Cells, Virus Replication (drug effects).
- MESH :
- chemical , genetics : Peptidyl-Dipeptidase A.
- drug effects : Gene Silencing, SARS Virus, Virus Replication.
- chemical , metabolism : Peptidyl-Dipeptidase A.
- chemical , pharmacology : RNA, Small Interfering.
- physiology : SARS Virus.
- Animals, Cats, Cell Line, Chlorocebus aethiops, Vero Cells.
Abstract
Abstract: The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.
Url:
- https://api.istex.fr/ark:/67375/VQC-KNR1MLT8-0/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088151
DOI: 10.1007/s10096-008-0495-5
Affiliations:
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Le document en format XML
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<term>Chlorocebus aethiops</term>
<term>Coronavirus</term>
<term>Gene Silencing (drug effects)</term>
<term>Microbiology</term>
<term>Peptidyl-Dipeptidase A (genetics)</term>
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<term>Peptidyl-Dipeptidase A (génétique)</term>
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<term>Virus du SRAS ()</term>
<term>Virus du SRAS (physiologie)</term>
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<front><div type="abstract" xml:lang="en">Abstract: The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.</div>
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