SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells.
Identifieur interne : 003032 ( Main/Exploration ); précédent : 003031; suivant : 003033SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells.
Auteurs : Eric C. Mossel [États-Unis] ; Jieru Wang ; Scott Jeffers ; Karen E. Edeen ; Shuanglin Wang ; Gregory P. Cosgrove ; C Joel Funk ; Rizwan Manzer ; Tanya A. Miura ; Leonard D. Pearson ; Kathryn V. Holmes ; Robert J. MasonSource :
- Virology [ 0042-6822 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- cytologie : Alvéoles pulmonaires.
- génétique : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Alvéoles pulmonaires, Épithélium.
- Cellules cultivées, Différenciation cellulaire, Effet cytopathogène viral, Humains, RT-PCR, Réplication virale, Virologie.
English descriptors
- KwdEn :
- Cell Differentiation, Cells, Cultured, Cytopathogenic Effect, Viral, Epithelium (virology), Humans, Pulmonary Alveoli (cytology), Pulmonary Alveoli (virology), RNA, Viral (analysis), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (physiology), Virology (methods), Virus Replication.
- MESH :
- chemical , analysis : RNA, Viral.
- cytology : Pulmonary Alveoli.
- genetics : SARS Virus.
- methods : Virology.
- physiology : SARS Virus.
- virology : Epithelium, Pulmonary Alveoli.
- Cell Differentiation, Cells, Cultured, Cytopathogenic Effect, Viral, Humans, Reverse Transcriptase Polymerase Chain Reaction, Virus Replication.
Abstract
Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 10(5) pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection.
DOI: 10.1016/j.virol.2007.09.045
PubMed: 18022664
Affiliations:
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Le document en format XML
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Mossel</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, Fort Collins, CO 80523, USA. Eric_Mossel@merck.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Colorado State University, Fort Collins, CO 80523</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Wang, Jieru" sort="Wang, Jieru" uniqKey="Wang J" first="Jieru" last="Wang">Jieru Wang</name></author><author><name sortKey="Jeffers, Scott" sort="Jeffers, Scott" uniqKey="Jeffers S" first="Scott" last="Jeffers">Scott Jeffers</name></author><author><name sortKey="Edeen, Karen E" sort="Edeen, Karen E" uniqKey="Edeen K" first="Karen E" last="Edeen">Karen E. Edeen</name></author><author><name sortKey="Wang, Shuanglin" sort="Wang, Shuanglin" uniqKey="Wang S" first="Shuanglin" last="Wang">Shuanglin Wang</name></author><author><name sortKey="Cosgrove, Gregory P" sort="Cosgrove, Gregory P" uniqKey="Cosgrove G" first="Gregory P" last="Cosgrove">Gregory P. Cosgrove</name></author><author><name sortKey="Funk, C Joel" sort="Funk, C Joel" uniqKey="Funk C" first="C Joel" last="Funk">C Joel Funk</name></author><author><name sortKey="Manzer, Rizwan" sort="Manzer, Rizwan" uniqKey="Manzer R" first="Rizwan" last="Manzer">Rizwan Manzer</name></author><author><name sortKey="Miura, Tanya A" sort="Miura, Tanya A" uniqKey="Miura T" first="Tanya A" last="Miura">Tanya A. Miura</name></author><author><name sortKey="Pearson, Leonard D" sort="Pearson, Leonard D" uniqKey="Pearson L" first="Leonard D" last="Pearson">Leonard D. Pearson</name></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name></author><author><name sortKey="Mason, Robert J" sort="Mason, Robert J" uniqKey="Mason R" first="Robert J" last="Mason">Robert J. 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We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 10(5) pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Colorado</li></region></list><tree><noCountry><name sortKey="Cosgrove, Gregory P" sort="Cosgrove, Gregory P" uniqKey="Cosgrove G" first="Gregory P" last="Cosgrove">Gregory P. Cosgrove</name><name sortKey="Edeen, Karen E" sort="Edeen, Karen E" uniqKey="Edeen K" first="Karen E" last="Edeen">Karen E. Edeen</name><name sortKey="Funk, C Joel" sort="Funk, C Joel" uniqKey="Funk C" first="C Joel" last="Funk">C Joel Funk</name><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name><name sortKey="Jeffers, Scott" sort="Jeffers, Scott" uniqKey="Jeffers S" first="Scott" last="Jeffers">Scott Jeffers</name><name sortKey="Manzer, Rizwan" sort="Manzer, Rizwan" uniqKey="Manzer R" first="Rizwan" last="Manzer">Rizwan Manzer</name><name sortKey="Mason, Robert J" sort="Mason, Robert J" uniqKey="Mason R" first="Robert J" last="Mason">Robert J. Mason</name><name sortKey="Miura, Tanya A" sort="Miura, Tanya A" uniqKey="Miura T" first="Tanya A" last="Miura">Tanya A. Miura</name><name sortKey="Pearson, Leonard D" sort="Pearson, Leonard D" uniqKey="Pearson L" first="Leonard D" last="Pearson">Leonard D. Pearson</name><name sortKey="Wang, Jieru" sort="Wang, Jieru" uniqKey="Wang J" first="Jieru" last="Wang">Jieru Wang</name><name sortKey="Wang, Shuanglin" sort="Wang, Shuanglin" uniqKey="Wang S" first="Shuanglin" last="Wang">Shuanglin Wang</name></noCountry><country name="États-Unis"><region name="Colorado"><name sortKey="Mossel, Eric C" sort="Mossel, Eric C" uniqKey="Mossel E" first="Eric C" last="Mossel">Eric C. Mossel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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