SrasV1, Main, Exploration, bibRecordById, pubmed:16101351

Profiles of IgG antibodies to nucleocapsid and spike proteins of the SARS-associated coronavirus in SARS patients.

Identifieur interne : 004712 ( Main/Exploration ); précédent : 004711; suivant : 004713

Profiles of IgG antibodies to nucleocapsid and spike proteins of the SARS-associated coronavirus in SARS patients.

Auteurs : Yanbin Wang [République populaire de Chine] ; Zhaorui Chang ; Jing Ouyang ; Haiyan Wei ; Renquan Yang ; Yangong Chao ; Jianguo Qu ; Jianwei Wang ; Tao Hung

Source :

DNA and cell biology [ 1044-5498 ] ; 2005.

RBID : pubmed:16101351

Descripteurs français

KwdFr :
- Anticorps antiviraux (sang), Escherichia coli (génétique), Escherichia coli (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Immunoglobuline G (sang), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Virus du SRAS (immunologie).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Escherichia coli.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : Escherichia coli.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Glycoprotéine de spicule des coronavirus, Humains.

English descriptors

KwdEn :
- Antibodies, Viral (blood), Escherichia coli (genetics), Escherichia coli (metabolism), Humans, Immunoglobulin G (blood), Membrane Glycoproteins (immunology), Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology).
MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Escherichia coli.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Recombinant Proteins, SARS Virus, Severe Acute Respiratory Syndrome, Viral Envelope Proteins.
- metabolism : Escherichia coli.
- Humans, Spike Glycoprotein, Coronavirus.

Abstract

To evaluate humoral immunity against the SARS-associated coronavirus (SARS-CoV), we studied the profiles of IgG antibodies to the nucleocapsid (N) and spike (S) proteins of SARS-CoV. Serum specimens from 10 SARS patients were analyzed by Western blotting and an enzyme-linked immunosorbent assay (ELISA) using purified recombinant N and truncated S (S1, S2, and S3) proteins as antigens. Western blotting results demonstrated that 100% of the SARS patients tested positive for N protein-specific antibodies, 50% for S1 protein-specific antibodies, 30% for S2 protein-specific antibodies, and 70% for S3 protein-specific antibodies. The ELISA results, which showed positive rates of IgG reactivity against recombinant proteins N, S1, S2, and S3, were, respectively, 28.57, 14.29, 14.29, and 14.29% at week 1, 77.78, 55.56, 44.44, and 66.67% at week 2, 100, 75, 75, and 87.5% at week 3, and 100, 77.78, 77.78, and 88.89% after 3 weeks. The average titers of IgG against recombinant proteins N, S1, S2, and S3 were, respectively, 691, 56, 38, and 84 after 3 weeks. These results suggest that the recombinant proteins N and S3 are potentially useful antigens for a serological diagnosis of SARS. In consideration of possible cross-reactivity among N proteins of SARS-CoV and other coronaviruses, immunoassays using recombinant N protein in combination with S3 as antigens might improve the specificity of SARS diagnoses.

DOI: 10.1089/dna.2005.24.521
PubMed: 16101351

Affiliations:

Le document en format XML

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<author><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name>
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<author><name sortKey="Hung, Tao" sort="Hung, Tao" uniqKey="Hung T" first="Tao" last="Hung">Tao Hung</name>
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<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (immunology)</term>
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<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Humains</term>
<term>Immunoglobuline G (sang)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
<term>Protéines recombinantes</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Recombinant Proteins</term>
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
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<front><div type="abstract" xml:lang="en">To evaluate humoral immunity against the SARS-associated coronavirus (SARS-CoV), we studied the profiles of IgG antibodies to the nucleocapsid (N) and spike (S) proteins of SARS-CoV. Serum specimens from 10 SARS patients were analyzed by Western blotting and an enzyme-linked immunosorbent assay (ELISA) using purified recombinant N and truncated S (S1, S2, and S3) proteins as antigens. Western blotting results demonstrated that 100% of the SARS patients tested positive for N protein-specific antibodies, 50% for S1 protein-specific antibodies, 30% for S2 protein-specific antibodies, and 70% for S3 protein-specific antibodies. The ELISA results, which showed positive rates of IgG reactivity against recombinant proteins N, S1, S2, and S3, were, respectively, 28.57, 14.29, 14.29, and 14.29% at week 1, 77.78, 55.56, 44.44, and 66.67% at week 2, 100, 75, 75, and 87.5% at week 3, and 100, 77.78, 77.78, and 88.89% after 3 weeks. The average titers of IgG against recombinant proteins N, S1, S2, and S3 were, respectively, 691, 56, 38, and 84 after 3 weeks. These results suggest that the recombinant proteins N and S3 are potentially useful antigens for a serological diagnosis of SARS. In consideration of possible cross-reactivity among N proteins of SARS-CoV and other coronaviruses, immunoassays using recombinant N protein in combination with S3 as antigens might improve the specificity of SARS diagnoses.</div>
</front>
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<name sortKey="Hung, Tao" sort="Hung, Tao" uniqKey="Hung T" first="Tao" last="Hung">Tao Hung</name>
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Profiles of IgG antibodies to nucleocapsid and spike proteins of the SARS-associated coronavirus in SARS patients.

Profiles of IgG antibodies to nucleocapsid and spike proteins of the SARS-associated coronavirus in SARS patients.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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