Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus.
Identifieur interne : 004726 ( Main/Exploration ); précédent : 004725; suivant : 004727Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus.
Auteurs : Xiaonan Fang [République populaire de Chine] ; Linbai Ye ; Khalid Amine Timani ; Shanshan Li ; Yingchun Zen ; Meng Zhao ; Hong Zheng ; Zhenghui WuSource :
- Journal of biochemistry and molecular biology [ 1225-8687 ] ; 2005.
Descripteurs français
- KwdFr :
- Assemblage viral, Cartographie d'interactions entre protéines, Données de séquences moléculaires, Fusion membranaire (physiologie), Glutathione transferase (génétique), Glutathione transferase (métabolisme), Liaison aux protéines, Protéines de la matrice virale (métabolisme), Protéines nucléocapside (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Similitude de séquences d'acides aminés, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS (métabolisme).
- MESH :
- génétique : Glutathione transferase, Protéines recombinantes.
- isolement et purification : Protéines recombinantes.
- métabolisme : Glutathione transferase, Protéines de la matrice virale, Protéines nucléocapside, Protéines recombinantes, Virus du SRAS.
- physiologie : Fusion membranaire.
- Assemblage viral, Cartographie d'interactions entre protéines, Données de séquences moléculaires, Liaison aux protéines, Similitude de séquences d'acides aminés, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Glutathione Transferase (genetics), Glutathione Transferase (metabolism), Membrane Fusion (physiology), Molecular Sequence Data, Nucleocapsid Proteins (metabolism), Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (metabolism), Sequence Homology, Amino Acid, Viral Matrix Proteins (metabolism), Virus Assembly.
- MESH :
- chemical , genetics : Glutathione Transferase, Recombinant Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- chemical , metabolism : Glutathione Transferase, Nucleocapsid Proteins, Recombinant Proteins, Viral Matrix Proteins.
- metabolism : SARS Virus.
- physiology : Membrane Fusion.
- Amino Acid Sequence, Molecular Sequence Data, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Virus Assembly.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.
DOI: 10.5483/bmbrep.2005.38.4.381
PubMed: 16053703
Affiliations:
Links toward previous steps (curation, corpus...)
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Le document en format XML
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Amine Timani</name></author><author><name sortKey="Li, Shanshan" sort="Li, Shanshan" uniqKey="Li S" first="Shanshan" last="Li">Shanshan Li</name></author><author><name sortKey="Zen, Yingchun" sort="Zen, Yingchun" uniqKey="Zen Y" first="Yingchun" last="Zen">Yingchun Zen</name></author><author><name sortKey="Zhao, Meng" sort="Zhao, Meng" uniqKey="Zhao M" first="Meng" last="Zhao">Meng Zhao</name></author><author><name sortKey="Zheng, Hong" sort="Zheng, Hong" uniqKey="Zheng H" first="Hong" last="Zheng">Hong Zheng</name></author><author><name sortKey="Wu, Zhenghui" sort="Wu, Zhenghui" uniqKey="Wu Z" first="Zhenghui" last="Wu">Zhenghui Wu</name></author></analytic><series><title level="j">Journal of biochemistry and molecular biology</title><idno type="ISSN">1225-8687</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Glutathione Transferase (genetics)</term><term>Glutathione Transferase (metabolism)</term><term>Membrane Fusion (physiology)</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (metabolism)</term><term>Protein Binding</term><term>Protein Interaction Mapping</term><term>Protein Structure, Tertiary</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (metabolism)</term><term>Sequence Homology, Amino Acid</term><term>Viral Matrix Proteins (metabolism)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Assemblage viral</term><term>Cartographie d'interactions entre protéines</term><term>Données de séquences moléculaires</term><term>Fusion membranaire (physiologie)</term><term>Glutathione transferase (génétique)</term><term>Glutathione transferase (métabolisme)</term><term>Liaison aux protéines</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines nucléocapside (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Similitude de séquences d'acides aminés</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutathione Transferase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Transferase</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutathione transferase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glutathione transferase</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fusion membranaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Membrane Fusion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Sequence Data</term><term>Protein Binding</term><term>Protein Interaction Mapping</term><term>Protein Structure, Tertiary</term><term>Sequence Homology, Amino Acid</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Assemblage viral</term><term>Cartographie d'interactions entre protéines</term><term>Données de séquences moléculaires</term><term>Liaison aux protéines</term><term>Similitude de séquences d'acides aminés</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Li, Shanshan" sort="Li, Shanshan" uniqKey="Li S" first="Shanshan" last="Li">Shanshan Li</name><name sortKey="Timani, Khalid Amine" sort="Timani, Khalid Amine" uniqKey="Timani K" first="Khalid Amine" last="Timani">Khalid Amine Timani</name><name sortKey="Wu, Zhenghui" sort="Wu, Zhenghui" uniqKey="Wu Z" first="Zhenghui" last="Wu">Zhenghui Wu</name><name sortKey="Ye, Linbai" sort="Ye, Linbai" uniqKey="Ye L" first="Linbai" last="Ye">Linbai Ye</name><name sortKey="Zen, Yingchun" sort="Zen, Yingchun" uniqKey="Zen Y" first="Yingchun" last="Zen">Yingchun Zen</name><name sortKey="Zhao, Meng" sort="Zhao, Meng" uniqKey="Zhao M" first="Meng" last="Zhao">Meng Zhao</name><name sortKey="Zheng, Hong" sort="Zheng, Hong" uniqKey="Zheng H" first="Hong" last="Zheng">Hong Zheng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Fang, Xiaonan" sort="Fang, Xiaonan" uniqKey="Fang X" first="Xiaonan" last="Fang">Xiaonan Fang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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