SrasV1, Main, Exploration, bibRecordById, pubmed:15752773

Growth kinetics of SARS-coronavirus in Vero E6 cells.

Identifieur interne : 004816 ( Main/Exploration ); précédent : 004815; suivant : 004817

Growth kinetics of SARS-coronavirus in Vero E6 cells.

Auteurs : Els Keyaerts [Belgique] ; Leen Vijgen ; Piet Maes ; Johan Neyts ; Marc Van Ranst

Source :

Biochemical and biophysical research communications [ 0006-291X ] ; 2005.

RBID : pubmed:15752773

Descripteurs français

KwdFr :
- ARN viral (analyse), Animaux, Cellules Vero (virologie), Numération de colonies microbiennes (), Prolifération cellulaire, Réplication virale (physiologie), Virus du SRAS (croissance et développement), Virus du SRAS (génétique).
MESH :
- analyse : ARN viral.
- croissance et développement : Virus du SRAS.
- génétique : Virus du SRAS.
- physiologie : Réplication virale.
- virologie : Cellules Vero.
- Animaux, Numération de colonies microbiennes, Prolifération cellulaire.

English descriptors

KwdEn :
- Animals, Cell Proliferation, Chlorocebus aethiops, Colony Count, Microbial (methods), RNA, Viral (analysis), SARS Virus (genetics), SARS Virus (growth & development), Vero Cells (virology), Virus Replication (physiology).
MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- growth & development : SARS Virus.
- methods : Colony Count, Microbial.
- physiology : Virus Replication.
- virology : Vero Cells.
- Animals, Cell Proliferation, Chlorocebus aethiops.

Abstract

Vero E6 cells are commonly used for in vitro studies of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and for antiviral evaluation purposes. A better understanding of the SARS-CoV growth kinetics in Vero E6 cells is crucial to help elucidate the mechanism of antiviral activity of selective antiviral agents. In this study, the growth kinetics of SARS-CoV in Vero E6 cells were studied by quantitation of intra- and extracellular viral RNA load as well as extracellular virus yield at different time points post-infection. At 12h post-infection, the intracellular viral RNA load was 3x10(2)-fold higher than at the time of infection, and the extracellular viral RNA load was increased with a factor of 2 x 10(3). Intracellular viral RNA levels started to rise at 6h post-infection. One hour later (at 7h post-infection), the levels of extracellular SARS-CoV RNA also began to rise. This was corroborated by the fact that infectious progeny SARS-CoV also first appeared in the supernatant between 6 and 7h post-infection. At 12h post-infection, SARS-CoV reached titers in the supernatant of 5.2 x 10(3) CCID(50)/ml.

DOI: 10.1016/j.bbrc.2005.02.085
PubMed: 15752773

Affiliations:

Belgique

Le document en format XML

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<front><div type="abstract" xml:lang="en">Vero E6 cells are commonly used for in vitro studies of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and for antiviral evaluation purposes. A better understanding of the SARS-CoV growth kinetics in Vero E6 cells is crucial to help elucidate the mechanism of antiviral activity of selective antiviral agents. In this study, the growth kinetics of SARS-CoV in Vero E6 cells were studied by quantitation of intra- and extracellular viral RNA load as well as extracellular virus yield at different time points post-infection. At 12h post-infection, the intracellular viral RNA load was 3x10(2)-fold higher than at the time of infection, and the extracellular viral RNA load was increased with a factor of 2 x 10(3). Intracellular viral RNA levels started to rise at 6h post-infection. One hour later (at 7h post-infection), the levels of extracellular SARS-CoV RNA also began to rise. This was corroborated by the fact that infectious progeny SARS-CoV also first appeared in the supernatant between 6 and 7h post-infection. At 12h post-infection, SARS-CoV reached titers in the supernatant of 5.2 x 10(3) CCID(50)/ml.</div>
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Growth kinetics of SARS-coronavirus in Vero E6 cells.

Growth kinetics of SARS-coronavirus in Vero E6 cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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