B-cell responses in patients who have recovered from severe acute respiratory syndrome target a dominant site in the S2 domain of the surface spike glycoprotein.
Identifieur interne : 004917 ( Main/Exploration ); précédent : 004916; suivant : 004918B-cell responses in patients who have recovered from severe acute respiratory syndrome target a dominant site in the S2 domain of the surface spike glycoprotein.
Auteurs : Xiaofen Zhong [République populaire de Chine] ; Huanghao Yang ; Zu-Feng Guo ; Wan-Yee Fion Sin ; Wei Chen ; Junjie Xu ; Ling Fu ; Jie Wu ; Chun-Kit Gannon Mak ; Chak-Sum Samuel Cheng ; Yanzhen Yang ; Shuyong Cao ; Tin-Yau Wong ; Sik-To Lai ; Yong Xie ; Zhihong GuoSource :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux (sang), Antigènes viraux (immunologie), Banque de peptides, Cartographie épitopique, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Hong Kong, Humains, Lymphocytes B (immunologie), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (immunologie), Protéines virales (immunologie), Protéines virales non structurales, Sujet âgé, Syndrome respiratoire aigu sévère (immunologie), Tests de neutralisation, Virus du SRAS (immunologie), Épitopes immunodominants.
- MESH :
- immunologie : Antigènes viraux, Glycoprotéines membranaires, Lymphocytes B, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines virales, Syndrome respiratoire aigu sévère, Virus du SRAS.
- sang : Anticorps antiviraux.
- Adulte, Adulte d'âge moyen, Banque de peptides, Cartographie épitopique, Glycoprotéine de spicule des coronavirus, Hong Kong, Humains, Protéines virales non structurales, Sujet âgé, Tests de neutralisation, Épitopes immunodominants.
- Wicri :
- geographic : Hong Kong.
English descriptors
- KwdEn :
- Adult, Aged, Antibodies, Viral (blood), Antigens, Viral (immunology), B-Lymphocytes (immunology), Epitope Mapping, Hong Kong, Humans, Immunodominant Epitopes, Membrane Glycoproteins (immunology), Middle Aged, Neutralization Tests, Nucleocapsid Proteins (immunology), Peptide Library, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology), Viral Nonstructural Proteins, Viral Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antigens, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Proteins.
- geographic : Hong Kong, Immunodominant Epitopes, Peptide Library, Spike Glycoprotein, Coronavirus, Viral Nonstructural Proteins.
- immunology : B-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome.
- Adult, Aged, Epitope Mapping, Humans, Middle Aged, Neutralization Tests.
Abstract
Severe acute respiratory syndrome (SARS) is a recently emerged infectious disease caused by a novel strain of coronavirus. Examination of the immune responses of patients who have recovered from SARS should provide important information for design of a safe and effective vaccine. We determined the continuous viral epitopes targeted by antibodies in plasma samples from convalescent SARS patients through biopanning with a vast M13 phage display dodecapeptide library. These epitopes converged to very short peptide fragments, one on each of the structural proteins spike and nucleocapsid and the nonstructural proteins 3a, 9b, and nsp 3. Immunoassays found that most of the patients who had recovered from SARS developed complementary antibodies to the epitope-rich region on the spike S2 protein, indicating that this is an immunodominant site on the viral envelope comprising the spike, matrix, and small envelope glycoproteins. These S2-targeting antibodies were shown to effectively neutralize the coronavirus, indicating that they provided protective immunity to help the patients recover from the viral infection. These results suggest that the SARS coronavirus might have an antigenic profile distinct from those of other human or animal coronaviruses. Due to the tested safety and protective effects of the convalescent-phase serological antibodies, identification of their complementary antigens may enable the design of an epitope-based vaccine to prevent potential antibody-mediated immunopathology.
DOI: 10.1128/JVI.79.6.3401-3408.2005
PubMed: 15731234
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a recently emerged infectious disease caused by a novel strain of coronavirus. Examination of the immune responses of patients who have recovered from SARS should provide important information for design of a safe and effective vaccine. We determined the continuous viral epitopes targeted by antibodies in plasma samples from convalescent SARS patients through biopanning with a vast M13 phage display dodecapeptide library. These epitopes converged to very short peptide fragments, one on each of the structural proteins spike and nucleocapsid and the nonstructural proteins 3a, 9b, and nsp 3. Immunoassays found that most of the patients who had recovered from SARS developed complementary antibodies to the epitope-rich region on the spike S2 protein, indicating that this is an immunodominant site on the viral envelope comprising the spike, matrix, and small envelope glycoproteins. These S2-targeting antibodies were shown to effectively neutralize the coronavirus, indicating that they provided protective immunity to help the patients recover from the viral infection. These results suggest that the SARS coronavirus might have an antigenic profile distinct from those of other human or animal coronaviruses. Due to the tested safety and protective effects of the convalescent-phase serological antibodies, identification of their complementary antigens may enable the design of an epitope-based vaccine to prevent potential antibody-mediated immunopathology.</div>
</front>
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<name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name>
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<name sortKey="Mak, Chun Kit Gannon" sort="Mak, Chun Kit Gannon" uniqKey="Mak C" first="Chun-Kit Gannon" last="Mak">Chun-Kit Gannon Mak</name>
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<name sortKey="Xu, Junjie" sort="Xu, Junjie" uniqKey="Xu J" first="Junjie" last="Xu">Junjie Xu</name>
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