Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability
Identifieur interne : 005355 ( Main/Exploration ); précédent : 005354; suivant : 005356Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability
Auteurs : Y. Liao [Singapour] ; J. Lescar [Singapour] ; J. P. Tam [Singapour] ; D. X. Liu [Singapour]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Analyse de mutations d'ADN, Animaux, Cellules HeLa, Conformation des protéines, Cystéine (métabolisme), Escherichia coli (génétique), Escherichia coli (physiologie), Humains, Mutagenèse dirigée, Perméabilité de la membrane cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Virus du SRAS (métabolisme).
- MESH :
- génétique : Escherichia coli, Protéines de l'enveloppe virale.
- métabolisme : Cystéine, Protéines de l'enveloppe virale, Virus du SRAS.
- physiologie : Escherichia coli.
- Analyse de mutations d'ADN, Animaux, Cellules HeLa, Conformation des protéines, Humains, Mutagenèse dirigée, Perméabilité de la membrane cellulaire, Protéines de l'enveloppe virale.
English descriptors
- KwdEn :
- Animals, Cell Membrane Permeability, Cysteine (metabolism), DNA Mutational Analysis, Escherichia coli (genetics), Escherichia coli (physiology), HeLa Cells, Humans, Mutagenesis, Site-Directed, Protein Conformation, SARS Virus (metabolism), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- chemical , metabolism : Cysteine, Viral Envelope Proteins.
- genetics : Escherichia coli.
- metabolism : SARS Virus.
- physiology : Escherichia coli.
- Animals, Cell Membrane Permeability, DNA Mutational Analysis, HeLa Cells, Humans, Mutagenesis, Site-Directed, Protein Conformation.
Abstract
To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome-associated coronavirus can induce membrane permeability changes when expressed in
Url:
DOI: 10.1016/j.bbrc.2004.10.050
PubMed: 15522242
PubMed Central: 7092894
Affiliations:
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Le document en format XML
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Liao</name><affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Lescar, J" sort="Lescar, J" uniqKey="Lescar J" first="J." last="Lescar">J. Lescar</name><affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Tam, J P" sort="Tam, J P" uniqKey="Tam J" first="J. P." last="Tam">J. P. Tam</name><affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D. X." last="Liu">D. X. Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>School of Biological Science, Nanyang Technological University, 61 Biopolis Drive, Proteos, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemical and Biophysical Research Communications</title><idno type="ISSN">0006-291X</idno><idno type="eISSN">1090-2104</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Membrane Permeability</term><term>Cysteine (metabolism)</term><term>DNA Mutational Analysis</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (physiology)</term><term>HeLa Cells</term><term>Humans</term><term>Mutagenesis, Site-Directed</term><term>Protein Conformation</term><term>SARS Virus (metabolism)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Conformation des protéines</term><term>Cystéine (métabolisme)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (physiologie)</term><term>Humains</term><term>Mutagenèse dirigée</term><term>Perméabilité de la membrane cellulaire</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cysteine</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cystéine</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Membrane Permeability</term><term>DNA Mutational Analysis</term><term>HeLa Cells</term><term>Humans</term><term>Mutagenesis, Site-Directed</term><term>Protein Conformation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Conformation des protéines</term><term>Humains</term><term>Mutagenèse dirigée</term><term>Perméabilité de la membrane cellulaire</term><term>Protéines de l'enveloppe virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome-associated coronavirus can induce membrane permeability changes when expressed in <italic>Escherichia coli</italic>. E protein expressed in bacterial and mammalian cells under reducing conditions existed as monomers, but formed homodimer and homotrimer under non-reducing conditions. Site-directed mutagenesis studies revealed that two cysteine residues of the E protein were essential for oligomerization, leading to induction of membrane permeability. This is the first report demonstrating that a coronavirus-encoded protein could modify membrane permeability in <italic>E. coli</italic> cells.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agirre, A" uniqKey="Agirre A">A. Agirre</name></author><author><name sortKey="Barco, A" uniqKey="Barco A">A. Barco</name></author><author><name sortKey="Carrasco, L" uniqKey="Carrasco L">L. 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Snijder</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H. Rabenau</name></author><author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W. Doerr</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><country name="Singapour"><noRegion><name sortKey="Liao, Y" sort="Liao, Y" uniqKey="Liao Y" first="Y." last="Liao">Y. Liao</name></noRegion><name sortKey="Lescar, J" sort="Lescar, J" uniqKey="Lescar J" first="J." last="Lescar">J. Lescar</name><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D. X." last="Liu">D. X. Liu</name><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D. X." last="Liu">D. X. Liu</name><name sortKey="Tam, J P" sort="Tam, J P" uniqKey="Tam J" first="J. 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