Immunogenicity of SARS inactivated vaccine in BALB/c mice.
Identifieur interne : 005303 ( Main/Exploration ); précédent : 005302; suivant : 005304Immunogenicity of SARS inactivated vaccine in BALB/c mice.
Auteurs : Sheng Xiong [République populaire de Chine] ; Yi-Fei Wang ; Mei-Ying Zhang ; Xin-Jian Liu ; Chuan-Hai Zhang ; Shi-Sheng Liu ; Chui-Wen Qian ; Jiu-Xiang Li ; Jia-Hai Lu ; Zhuo-Yue Wan ; Huan-Yin Zheng ; Xin-Ge Yan ; Min-Jie Meng ; Jiang-Lin FanSource :
- Immunology letters [ 0165-2478 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Antigènes viraux (immunologie), Cinétique, Immunoglobuline G (immunologie), Immunoglobuline G (sang), Souris, Souris de lignée BALB C, Spécificité des anticorps, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Sérums immuns (immunologie), Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins inactivés (immunologie), Virus du SRAS (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Antigènes viraux, Immunoglobuline G, Syndrome respiratoire aigu sévère, Sérums immuns, Vaccins antiviraux, Vaccins inactivés, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cinétique, Souris, Souris de lignée BALB C, Spécificité des anticorps, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibody Specificity, Antigens, Viral (immunology), Immune Sera (immunology), Immunoglobulin G (blood), Immunoglobulin G (immunology), Kinetics, Mice, Mice, Inbred BALB C, Neutralization Tests, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vaccines, Inactivated (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Immune Sera, Immunoglobulin G, Vaccines, Inactivated, Viral Vaccines.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Kinetics, Mice, Mice, Inbred BALB C, Neutralization Tests.
Abstract
Severe acute respiratory syndrome (SARS) is a serious infectious threat to public health. To create a novel trial vaccine and evaluate its potency, we attempted to generate a SARS inactivated vaccine using SARS coronavirus (SARS-CoV) strain F69 treated with formaldehyde and mixed with Al(OH)3. Three doses of the vaccine were used to challenge three groups of BALB/c mice. We found that the mice exhibited specific IgM on day 4 and IgG on day 8. The peak titers of IgG were at day 47 in low-dose group (1:19,200) and high-dose group (1:38,400) whereas in middle-dose group (1:19,200), the peak was at day 40. On day 63, the IgG levels reached a plateau. Neutralization assay demonstrated that the antisera could protect Vero-E6 cells from SARS-CoV's infection. Analysis of the antibody specificity revealed that the mouse antisera contained a mixture of antibodies specifically against the structure proteins of SARS-CoV. Furthermore, the mouse antisera conferred higher amount of antibodies against protein N, polypeptide S4 and S2 than those of proteins M and 3CL. These findings suggest that the inactivated SARS-CoV could preserve its antigenicity and the inactivated vaccine can stimulate mice to produce high levels of antibodies with neutralization activity. Results also suggest that polypeptides originating from protein N or S might be a potential target for the generation of a recombinant SARS vaccine.
DOI: 10.1016/j.imlet.2004.06.014
PubMed: 15388253
Affiliations:
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Le document en format XML
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<term>Antigens, Viral (immunology)</term>
<term>Immune Sera (immunology)</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin G (immunology)</term>
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<term>Severe Acute Respiratory Syndrome (immunology)</term>
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<term>Immunoglobuline G (immunologie)</term>
<term>Immunoglobuline G (sang)</term>
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<term>Souris de lignée BALB C</term>
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<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Sérums immuns (immunologie)</term>
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<term>Vaccins antiviraux (immunologie)</term>
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<term>Immunoglobulin G</term>
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<term>Immune Sera</term>
<term>Immunoglobulin G</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a serious infectious threat to public health. To create a novel trial vaccine and evaluate its potency, we attempted to generate a SARS inactivated vaccine using SARS coronavirus (SARS-CoV) strain F69 treated with formaldehyde and mixed with Al(OH)3. Three doses of the vaccine were used to challenge three groups of BALB/c mice. We found that the mice exhibited specific IgM on day 4 and IgG on day 8. The peak titers of IgG were at day 47 in low-dose group (1:19,200) and high-dose group (1:38,400) whereas in middle-dose group (1:19,200), the peak was at day 40. On day 63, the IgG levels reached a plateau. Neutralization assay demonstrated that the antisera could protect Vero-E6 cells from SARS-CoV's infection. Analysis of the antibody specificity revealed that the mouse antisera contained a mixture of antibodies specifically against the structure proteins of SARS-CoV. Furthermore, the mouse antisera conferred higher amount of antibodies against protein N, polypeptide S4 and S2 than those of proteins M and 3CL. These findings suggest that the inactivated SARS-CoV could preserve its antigenicity and the inactivated vaccine can stimulate mice to produce high levels of antibodies with neutralization activity. Results also suggest that polypeptides originating from protein N or S might be a potential target for the generation of a recombinant SARS vaccine.</div>
</front>
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<name sortKey="Liu, Shi Sheng" sort="Liu, Shi Sheng" uniqKey="Liu S" first="Shi-Sheng" last="Liu">Shi-Sheng Liu</name>
<name sortKey="Liu, Xin Jian" sort="Liu, Xin Jian" uniqKey="Liu X" first="Xin-Jian" last="Liu">Xin-Jian Liu</name>
<name sortKey="Lu, Jia Hai" sort="Lu, Jia Hai" uniqKey="Lu J" first="Jia-Hai" last="Lu">Jia-Hai Lu</name>
<name sortKey="Meng, Min Jie" sort="Meng, Min Jie" uniqKey="Meng M" first="Min-Jie" last="Meng">Min-Jie Meng</name>
<name sortKey="Qian, Chui Wen" sort="Qian, Chui Wen" uniqKey="Qian C" first="Chui-Wen" last="Qian">Chui-Wen Qian</name>
<name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name>
<name sortKey="Wang, Yi Fei" sort="Wang, Yi Fei" uniqKey="Wang Y" first="Yi-Fei" last="Wang">Yi-Fei Wang</name>
<name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name>
<name sortKey="Zhang, Chuan Hai" sort="Zhang, Chuan Hai" uniqKey="Zhang C" first="Chuan-Hai" last="Zhang">Chuan-Hai Zhang</name>
<name sortKey="Zhang, Mei Ying" sort="Zhang, Mei Ying" uniqKey="Zhang M" first="Mei-Ying" last="Zhang">Mei-Ying Zhang</name>
<name sortKey="Zheng, Huan Yin" sort="Zheng, Huan Yin" uniqKey="Zheng H" first="Huan-Yin" last="Zheng">Huan-Yin Zheng</name>
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<country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Xiong, Sheng" sort="Xiong, Sheng" uniqKey="Xiong S" first="Sheng" last="Xiong">Sheng Xiong</name>
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