Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR
Identifieur interne : 005B43 ( Main/Exploration ); précédent : 005B42; suivant : 005B44Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR
Auteurs : Laura Gillim-Ross [États-Unis] ; Jill Taylor [États-Unis] ; David R. Scholl [États-Unis] ; Jared Ridenour [États-Unis] ; Paul S. Masters [États-Unis] ; David E. Wentworth [États-Unis]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Récepteurs viraux.
- physiologie : Virus du SRAS.
- virologie : Rein.
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Animals, CD13 Antigens (physiology), Cell Line, Coronavirus, Haplorhini, Human, Humans, Infected cell, Kidney (virology), Microbiology, Multiplex polymerase chain reaction, Receptors, Virus (analysis), Replication, Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse transcription polymerase chain reaction, SARS Virus (physiology), Severe acute respiratory syndrome, Virus Replication.
- MESH :
- chemical , analysis : Receptors, Virus.
- chemical , physiology : CD13 Antigens.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- physiology : SARS Virus.
- virology : Kidney.
- Animals, Cell Line, Haplorhini, Humans, Virus Replication.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) is the causative agent of the recent outbreak of severe acute respiratory syndrome. VeroE6 cells, fetal rhesus monkey kidney cells, and human peripheral blood mononuclear cells were the only cells known to be susceptible to SARS-CoV. We developed a multiplex reverse transcription-PCR assay to analyze the susceptibility of cells derived from a variety of tissues and species to SARS-CoV. Additionally, productive infection was determined by titration of cellular supernatants. Cells derived from three species of monkey were susceptible to SARS-CoV. However, the levels of SARS-CoV produced differed by 4 log10. Mink lung epithelial cells (MvlLu) and R-Mix, a mixed monolayer of human lung-derived cells (A549) and mink lung-derived cells (MvlLu), are used by diagnostic laboratories to detect respiratory viruses (e.g., influenza virus); they were also infected with SARS-CoV, indicating that the practices of diagnostic laboratories should be examined to ensure appropriate biosafety precautions. MvlLu cells produce little SARS-CoV compared to that produced by VeroE6 cells, which indicates that they are a safer alternative for SARS-CoV diagnostics. Evaluation of cells permissive to other coronaviruses indicated that these cell types are not infected by SARS-CoV, providing additional evidence that SARS-CoV binds an alternative receptor. Analysis of human cells derived from lung, kidney, liver, and intestine led to the discovery that human cell lines were productively infected by SARS-CoV. This study identifies new cell lines that may be used for SARS-CoV diagnostics and/or basic research. Our data and other in vivo studies indicate that SARS-CoV has a wide host range, suggesting that the cellular receptor(s) utilized by SARS-CoV is highly conserved and is expressed by a variety of tissues.
Affiliations:
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Scholl</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Diagnostic Hybrids, Inc.</s1><s2>Athens, Ohio 45701</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Athens, Ohio 45701</wicri:noRegion></affiliation></author><author><name sortKey="Ridenour, Jared" sort="Ridenour, Jared" uniqKey="Ridenour J" first="Jared" last="Ridenour">Jared Ridenour</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Diagnostic Hybrids, Inc.</s1><s2>Athens, Ohio 45701</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Athens, Ohio 45701</wicri:noRegion></affiliation></author><author><name sortKey="Masters, Paul S" sort="Masters, Paul S" uniqKey="Masters P" first="Paul S." last="Masters">Paul S. Masters</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wadsworth Center, New York State Department of Health, State University of New York</s1><s2>Albany, New York 12002</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Albany, New York 12002</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences, State University of New York</s1><s2>Albany, New York 12002</s2><s3>USA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Albany, New York 12002</wicri:noRegion></affiliation></author><author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wadsworth Center, New York State Department of Health, State University of New York</s1><s2>Albany, New York 12002</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Albany, New York 12002</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences, State University of New York</s1><s2>Albany, New York 12002</s2><s3>USA</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Albany, New York 12002</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>CD13 Antigens (physiology)</term><term>Cell Line</term><term>Coronavirus</term><term>Haplorhini</term><term>Human</term><term>Humans</term><term>Infected cell</term><term>Kidney (virology)</term><term>Microbiology</term><term>Multiplex polymerase chain reaction</term><term>Receptors, Virus (analysis)</term><term>Replication</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Reverse transcription polymerase chain reaction</term><term>SARS Virus (physiology)</term><term>Severe acute respiratory syndrome</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Haplorhini</term><term>Humains</term><term>Lignée cellulaire</term><term>RT-PCR ()</term><term>Rein (virologie)</term><term>Récepteurs viraux (analyse)</term><term>Réplication virale</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>CD13 Antigens</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Rein</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Kidney</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Haplorhini</term><term>Humans</term><term>Virus Replication</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Haplorhini</term><term>Homme</term><term>Coronavirus</term><term>Cellule infectée</term><term>Humains</term><term>Lignée cellulaire</term><term>RT-PCR</term><term>Réplication</term><term>Réaction chaîne polymérase multiplex</term><term>Réaction chaîne polymérase RT</term><term>Microbiologie</term><term>Réplication virale</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) is the causative agent of the recent outbreak of severe acute respiratory syndrome. VeroE6 cells, fetal rhesus monkey kidney cells, and human peripheral blood mononuclear cells were the only cells known to be susceptible to SARS-CoV. We developed a multiplex reverse transcription-PCR assay to analyze the susceptibility of cells derived from a variety of tissues and species to SARS-CoV. Additionally, productive infection was determined by titration of cellular supernatants. Cells derived from three species of monkey were susceptible to SARS-CoV. However, the levels of SARS-CoV produced differed by 4 log<sub>10</sub>. Mink lung epithelial cells (MvlLu) and R-Mix, a mixed monolayer of human lung-derived cells (A549) and mink lung-derived cells (MvlLu), are used by diagnostic laboratories to detect respiratory viruses (e.g., influenza virus); they were also infected with SARS-CoV, indicating that the practices of diagnostic laboratories should be examined to ensure appropriate biosafety precautions. MvlLu cells produce little SARS-CoV compared to that produced by VeroE6 cells, which indicates that they are a safer alternative for SARS-CoV diagnostics. Evaluation of cells permissive to other coronaviruses indicated that these cell types are not infected by SARS-CoV, providing additional evidence that SARS-CoV binds an alternative receptor. Analysis of human cells derived from lung, kidney, liver, and intestine led to the discovery that human cell lines were productively infected by SARS-CoV. This study identifies new cell lines that may be used for SARS-CoV diagnostics and/or basic research. Our data and other in vivo studies indicate that SARS-CoV has a wide host range, suggesting that the cellular receptor(s) utilized by SARS-CoV is highly conserved and is expressed by a variety of tissues.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Gillim Ross, Laura" sort="Gillim Ross, Laura" uniqKey="Gillim Ross L" first="Laura" last="Gillim-Ross">Laura Gillim-Ross</name></noRegion><name sortKey="Masters, Paul S" sort="Masters, Paul S" uniqKey="Masters P" first="Paul S." last="Masters">Paul S. Masters</name><name sortKey="Masters, Paul S" sort="Masters, Paul S" uniqKey="Masters P" first="Paul S." last="Masters">Paul S. Masters</name><name sortKey="Ridenour, Jared" sort="Ridenour, Jared" uniqKey="Ridenour J" first="Jared" last="Ridenour">Jared Ridenour</name><name sortKey="Scholl, David R" sort="Scholl, David R" uniqKey="Scholl D" first="David R." last="Scholl">David R. Scholl</name><name sortKey="Taylor, Jill" sort="Taylor, Jill" uniqKey="Taylor J" first="Jill" last="Taylor">Jill Taylor</name><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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