SrasV1, Main, Exploration, bibRecordById, pubmed:15242944

Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.

Identifieur interne : 005366 ( Main/Exploration ); précédent : 005365; suivant : 005367

Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.

Auteurs : Ming Guan [Singapour] ; Kwok Hung Chan ; J S Malik Peiris ; See Wai Kwan ; Siu Yan Lam ; Chiu Mei Pang ; Ka Wing Chu ; Kit Man Chan ; Hsiao Ying Chen ; Ewe Beng Phuah ; Caiqin Jane Wong

Source :

Clinical and diagnostic laboratory immunology [ 1071-412X ] ; 2004.

RBID : pubmed:15242944

Descripteurs français

KwdFr :
- Anticorps antiviraux (sang), Chromatographie (), Facteurs temps, Humains, Immunoglobuline G (sang), Normes de référence, Protéines recombinantes (immunologie), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (sang), Technique d'immunofluorescence, Test ELISA (), Tests sérologiques (normes), Virus du SRAS (immunologie).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Protéines recombinantes, Virus du SRAS.
- normes : Tests sérologiques.
- sang : Anticorps antiviraux, Immunoglobuline G, Syndrome respiratoire aigu sévère.
- Chromatographie, Facteurs temps, Humains, Normes de référence, Sensibilité et spécificité, Technique d'immunofluorescence, Test ELISA.

English descriptors

KwdEn :
- Antibodies, Viral (blood), Chromatography (methods), Enzyme-Linked Immunosorbent Assay (methods), Fluorescent Antibody Technique, Humans, Immunoglobulin G (blood), Recombinant Proteins (immunology), Reference Standards, SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (standards), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis), Time Factors.
MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Recombinant Proteins.
- blood : Severe Acute Respiratory Syndrome.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Chromatography, Enzyme-Linked Immunosorbent Assay.
- standards : Serologic Tests.
- Fluorescent Antibody Technique, Humans, Reference Standards, Sensitivity and Specificity, Time Factors.

Abstract

A newly developed severe acute respiratory syndrome (SARS)-specific enzyme-linked immunosorbent assay (ELISA) was further validated to confirm cutoff values and evaluate its diagnostic performance with clinical samples. In parallel, an immunochromatographic test was also evaluated. A total of 227 clinical serum specimens collected from SARS patients were used in the study, together with 385 samples from healthy donors. By use of an immunofluorescent (IF) test as the "gold standard", both the ELISA and the immunochromatographic test were able to detect immunoglobulin G antibodies to SARS not only from late-convalescent-stage samples (>21 days from the onset of clinical symptoms), as previously established, but also from early-acute-phase samples (1 to 10 days from onset). The ELISA, using an optical density (OD) of 0.25 as its cutoff value, produced the best sensitivity while maintaining high specificity. It detected SARS-specific antibodies in 58, 70, 75, and 95%, respectively, of the four groups of samples collected from patients 1 to 10 days, 11 to 20 days, 21 to 30 days, and more than 30 days after the onset of clinical symptoms. Similarly, the immunochromatographic test detected SARS-specific antibodies in 55, 68, 81, and 79% of the four groups, respectively. The overall specificities for the ELISA and the rapid test were 99.5 and 97.7%, respectively. Although the positive correlation observed between the ELISA OD values and the IF titers was moderate (r = 0.6915; P < 0.001), the detection rates of both the ELISA and the rapid test were found well in agreement with the IF titers.

DOI: 10.1128/CDLI.11.4.699-703.2004
PubMed: 15242944

Affiliations:

Singapour

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.</title>
<author><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name>
<affiliation wicri:level="1"><nlm:affiliation>Genelabs Diagnostics Pte Ltd., Singapore, Republic of Singapore. guanming@mail.genelabs.com.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name>
</author>
<author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name>
</author>
<author><name sortKey="Kwan, See Wai" sort="Kwan, See Wai" uniqKey="Kwan S" first="See Wai" last="Kwan">See Wai Kwan</name>
</author>
<author><name sortKey="Lam, Siu Yan" sort="Lam, Siu Yan" uniqKey="Lam S" first="Siu Yan" last="Lam">Siu Yan Lam</name>
</author>
<author><name sortKey="Pang, Chiu Mei" sort="Pang, Chiu Mei" uniqKey="Pang C" first="Chiu Mei" last="Pang">Chiu Mei Pang</name>
</author>
<author><name sortKey="Chu, Ka Wing" sort="Chu, Ka Wing" uniqKey="Chu K" first="Ka Wing" last="Chu">Ka Wing Chu</name>
</author>
<author><name sortKey="Chan, Kit Man" sort="Chan, Kit Man" uniqKey="Chan K" first="Kit Man" last="Chan">Kit Man Chan</name>
</author>
<author><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name>
</author>
<author><name sortKey="Phuah, Ewe Beng" sort="Phuah, Ewe Beng" uniqKey="Phuah E" first="Ewe Beng" last="Phuah">Ewe Beng Phuah</name>
</author>
<author><name sortKey="Wong, Caiqin Jane" sort="Wong, Caiqin Jane" uniqKey="Wong C" first="Caiqin Jane" last="Wong">Caiqin Jane Wong</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15242944</idno>
<idno type="pmid">15242944</idno>
<idno type="doi">10.1128/CDLI.11.4.699-703.2004</idno>
<idno type="wicri:Area/PubMed/Corpus">002C73</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C73</idno>
<idno type="wicri:Area/PubMed/Curation">002C73</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C73</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002D19</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D19</idno>
<idno type="wicri:Area/Ncbi/Merge">000937</idno>
<idno type="wicri:Area/Ncbi/Curation">000937</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000937</idno>
<idno type="wicri:doubleKey">1071-412X:2004:Guan M:evaluation:and:validation</idno>
<idno type="wicri:Area/Main/Merge">005786</idno>
<idno type="wicri:Area/Main/Curation">005366</idno>
<idno type="wicri:Area/Main/Exploration">005366</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.</title>
<author><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name>
<affiliation wicri:level="1"><nlm:affiliation>Genelabs Diagnostics Pte Ltd., Singapore, Republic of Singapore. guanming@mail.genelabs.com.sg</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
</affiliation>
</author>
<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name>
</author>
<author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name>
</author>
<author><name sortKey="Kwan, See Wai" sort="Kwan, See Wai" uniqKey="Kwan S" first="See Wai" last="Kwan">See Wai Kwan</name>
</author>
<author><name sortKey="Lam, Siu Yan" sort="Lam, Siu Yan" uniqKey="Lam S" first="Siu Yan" last="Lam">Siu Yan Lam</name>
</author>
<author><name sortKey="Pang, Chiu Mei" sort="Pang, Chiu Mei" uniqKey="Pang C" first="Chiu Mei" last="Pang">Chiu Mei Pang</name>
</author>
<author><name sortKey="Chu, Ka Wing" sort="Chu, Ka Wing" uniqKey="Chu K" first="Ka Wing" last="Chu">Ka Wing Chu</name>
</author>
<author><name sortKey="Chan, Kit Man" sort="Chan, Kit Man" uniqKey="Chan K" first="Kit Man" last="Chan">Kit Man Chan</name>
</author>
<author><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name>
</author>
<author><name sortKey="Phuah, Ewe Beng" sort="Phuah, Ewe Beng" uniqKey="Phuah E" first="Ewe Beng" last="Phuah">Ewe Beng Phuah</name>
</author>
<author><name sortKey="Wong, Caiqin Jane" sort="Wong, Caiqin Jane" uniqKey="Wong C" first="Caiqin Jane" last="Wong">Caiqin Jane Wong</name>
</author>
</analytic>
<series><title level="j">Clinical and diagnostic laboratory immunology</title>
<idno type="ISSN">1071-412X</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term>
<term>Chromatography (methods)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Fluorescent Antibody Technique</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reference Standards</term>
<term>SARS Virus (immunology)</term>
<term>Sensitivity and Specificity</term>
<term>Serologic Tests (standards)</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term>
<term>Chromatographie ()</term>
<term>Facteurs temps</term>
<term>Humains</term>
<term>Immunoglobuline G (sang)</term>
<term>Normes de référence</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Sensibilité et spécificité</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (sang)</term>
<term>Technique d'immunofluorescence</term>
<term>Test ELISA ()</term>
<term>Tests sérologiques (normes)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Tests sérologiques</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Serologic Tests</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Fluorescent Antibody Technique</term>
<term>Humans</term>
<term>Reference Standards</term>
<term>Sensitivity and Specificity</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Chromatographie</term>
<term>Facteurs temps</term>
<term>Humains</term>
<term>Normes de référence</term>
<term>Sensibilité et spécificité</term>
<term>Technique d'immunofluorescence</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A newly developed severe acute respiratory syndrome (SARS)-specific enzyme-linked immunosorbent assay (ELISA) was further validated to confirm cutoff values and evaluate its diagnostic performance with clinical samples. In parallel, an immunochromatographic test was also evaluated. A total of 227 clinical serum specimens collected from SARS patients were used in the study, together with 385 samples from healthy donors. By use of an immunofluorescent (IF) test as the "gold standard", both the ELISA and the immunochromatographic test were able to detect immunoglobulin G antibodies to SARS not only from late-convalescent-stage samples (>21 days from the onset of clinical symptoms), as previously established, but also from early-acute-phase samples (1 to 10 days from onset). The ELISA, using an optical density (OD) of 0.25 as its cutoff value, produced the best sensitivity while maintaining high specificity. It detected SARS-specific antibodies in 58, 70, 75, and 95%, respectively, of the four groups of samples collected from patients 1 to 10 days, 11 to 20 days, 21 to 30 days, and more than 30 days after the onset of clinical symptoms. Similarly, the immunochromatographic test detected SARS-specific antibodies in 55, 68, 81, and 79% of the four groups, respectively. The overall specificities for the ELISA and the rapid test were 99.5 and 97.7%, respectively. Although the positive correlation observed between the ELISA OD values and the IF titers was moderate (r = 0.6915; P < 0.001), the detection rates of both the ELISA and the rapid test were found well in agreement with the IF titers.</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
</country>
</list>
<tree><noCountry><name sortKey="Chan, Kit Man" sort="Chan, Kit Man" uniqKey="Chan K" first="Kit Man" last="Chan">Kit Man Chan</name>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name>
<name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name>
<name sortKey="Chu, Ka Wing" sort="Chu, Ka Wing" uniqKey="Chu K" first="Ka Wing" last="Chu">Ka Wing Chu</name>
<name sortKey="Kwan, See Wai" sort="Kwan, See Wai" uniqKey="Kwan S" first="See Wai" last="Kwan">See Wai Kwan</name>
<name sortKey="Lam, Siu Yan" sort="Lam, Siu Yan" uniqKey="Lam S" first="Siu Yan" last="Lam">Siu Yan Lam</name>
<name sortKey="Pang, Chiu Mei" sort="Pang, Chiu Mei" uniqKey="Pang C" first="Chiu Mei" last="Pang">Chiu Mei Pang</name>
<name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name>
<name sortKey="Phuah, Ewe Beng" sort="Phuah, Ewe Beng" uniqKey="Phuah E" first="Ewe Beng" last="Phuah">Ewe Beng Phuah</name>
<name sortKey="Wong, Caiqin Jane" sort="Wong, Caiqin Jane" uniqKey="Wong C" first="Caiqin Jane" last="Wong">Caiqin Jane Wong</name>
</noCountry>
<country name="Singapour"><noRegion><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005366 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005366 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:15242944
   |texte=   Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:15242944" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.

Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.