SrasV1, Main, Exploration, bibRecordById, pubmed:15049359

One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus

Identifieur interne : 005A97 ( Main/Exploration ); précédent : 005A96; suivant : 005A98

One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus

Auteurs : Sheng-Ce Tao [République populaire de Chine] ; DI JIANG [République populaire de Chine] ; Hong-Li Lu [République populaire de Chine] ; Wan-Li Xing [République populaire de Chine] ; Yu-Xiang Zhou [République populaire de Chine] ; JING CHENG [République populaire de Chine]

Source :

Biotechnology letters [ 0141-5492 ] ; 2004.

RBID : Pascal:04-0257195

Descripteurs français

KwdFr :
- ARN viral (analyse), ARN viral (génétique), Analyse de panne d'appareillage, Conception d'appareillage, Matériaux biocompatibles, Polyéthylènes, RT-PCR (), RT-PCR (instrumentation), Reproductibilité des résultats, Sensibilité et spécificité, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- analyse : ARN viral.
- génétique : ARN viral, Virus du SRAS.
- isolement et purification : Virus du SRAS.
Pascal (Inist)
- Analyse de panne d'appareillage, Conception d'appareillage, Matériaux biocompatibles, Polyéthylènes, RT-PCR, Reproductibilité des résultats, Réaction chaîne polymérase, Application, Sensibilité et spécificité, Technique rapide, Détection, Virus.

English descriptors

KwdEn :
- Application, Biocompatible Materials, Detection, Equipment Design, Equipment Failure Analysis, Polyethylenes, Polymerase chain reaction, RNA, Viral (analysis), RNA, Viral (genetics), Rapid technique, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (instrumentation), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Virus.
MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical : Biocompatible Materials, Polyethylenes.
- genetics : SARS Virus.
- instrumentation : Reverse Transcriptase Polymerase Chain Reaction.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Equipment Design, Equipment Failure Analysis, Reproducibility of Results, Sensitivity and Specificity.

Abstract

A general strategy based on the use of a plastic film that allows reverse transcription and nested-PCR in a single closed-tube has been developed. The reaction mixture for the second PCR amplification is quarantined in the cap of the reaction tube during the first round amplification by a piece of plastic film, and later introduced into the PCR amplicons from the first round reaction by centrifugation without opening the reaction tube. The main advantages of our method are its high sensitivity, specificity, simplicity, cost effectiveness, low risk of contamination and the ease in establishment of conditions for nested-PCR. The method has been successfully applied to the detection of the genomic RNA from SARS-CoV, the detection limit of this method is comparable to that of the conventional two-tube nested PCR system. This method could be easily adapted to the detection of other targets.

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus</title>
<author><name sortKey="Tao, Sheng Ce" sort="Tao, Sheng Ce" uniqKey="Tao S" first="Sheng-Ce" last="Tao">Sheng-Ce Tao</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Jiang" sort="Di Jiang" uniqKey="Di Jiang" last="Di Jiang">DI JIANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lu, Hong Li" sort="Lu, Hong Li" uniqKey="Lu H" first="Hong-Li" last="Lu">Hong-Li Lu</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Xing, Wan Li" sort="Xing, Wan Li" uniqKey="Xing W" first="Wan-Li" last="Xing">Wan-Li Xing</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhou, Yu Xiang" sort="Zhou, Yu Xiang" uniqKey="Zhou Y" first="Yu-Xiang" last="Zhou">Yu-Xiang Zhou</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jing Cheng" sort="Jing Cheng" uniqKey="Jing Cheng" last="Jing Cheng">JING CHENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">04-0257195</idno>
<date when="2004">2004</date>
<idno type="stanalyst">PASCAL 04-0257195 INIST</idno>
<idno type="RBID">Pascal:04-0257195</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000904</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000086</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000804</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000804</idno>
<idno type="wicri:doubleKey">0141-5492:2004:Tao S:one:tube:nested</idno>
<idno type="wicri:Area/Main/Merge">005F55</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:15049359</idno>
<idno type="wicri:Area/PubMed/Corpus">002E76</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E76</idno>
<idno type="wicri:Area/PubMed/Curation">002E76</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E76</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002B67</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B67</idno>
<idno type="wicri:Area/Ncbi/Merge">000722</idno>
<idno type="wicri:Area/Ncbi/Curation">000722</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000722</idno>
<idno type="wicri:doubleKey">0141-5492:2004:Tao S:one:tube:nested</idno>
<idno type="wicri:Area/Main/Merge">005609</idno>
<idno type="wicri:Area/Main/Curation">005A97</idno>
<idno type="wicri:Area/Main/Exploration">005A97</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus</title>
<author><name sortKey="Tao, Sheng Ce" sort="Tao, Sheng Ce" uniqKey="Tao S" first="Sheng-Ce" last="Tao">Sheng-Ce Tao</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Di Jiang" sort="Di Jiang" uniqKey="Di Jiang" last="Di Jiang">DI JIANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lu, Hong Li" sort="Lu, Hong Li" uniqKey="Lu H" first="Hong-Li" last="Lu">Hong-Li Lu</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Xing, Wan Li" sort="Xing, Wan Li" uniqKey="Xing W" first="Wan-Li" last="Xing">Wan-Li Xing</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhou, Yu Xiang" sort="Zhou, Yu Xiang" uniqKey="Zhou Y" first="Yu-Xiang" last="Zhou">Yu-Xiang Zhou</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jing Cheng" sort="Jing Cheng" uniqKey="Jing Cheng" last="Jing Cheng">JING CHENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Haidian District, Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1>
<s2>Beijing 100084</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway</s1>
<s2>Changping District, Beijing 102206</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Biotechnology letters</title>
<title level="j" type="abbreviated">Biotechnol. lett.</title>
<idno type="ISSN">0141-5492</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Biotechnology letters</title>
<title level="j" type="abbreviated">Biotechnol. lett.</title>
<idno type="ISSN">0141-5492</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Application</term>
<term>Biocompatible Materials</term>
<term>Detection</term>
<term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Polyethylenes</term>
<term>Polymerase chain reaction</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>Rapid technique</term>
<term>Reproducibility of Results</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (instrumentation)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
<term>Virus</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>ARN viral (génétique)</term>
<term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Matériaux biocompatibles</term>
<term>Polyéthylènes</term>
<term>RT-PCR ()</term>
<term>RT-PCR (instrumentation)</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biocompatible Materials</term>
<term>Polyethylenes</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Matériaux biocompatibles</term>
<term>Polyéthylènes</term>
<term>RT-PCR</term>
<term>Reproductibilité des résultats</term>
<term>Réaction chaîne polymérase</term>
<term>Application</term>
<term>Sensibilité et spécificité</term>
<term>Technique rapide</term>
<term>Détection</term>
<term>Virus</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A general strategy based on the use of a plastic film that allows reverse transcription and nested-PCR in a single closed-tube has been developed. The reaction mixture for the second PCR amplification is quarantined in the cap of the reaction tube during the first round amplification by a piece of plastic film, and later introduced into the PCR amplicons from the first round reaction by centrifugation without opening the reaction tube. The main advantages of our method are its high sensitivity, specificity, simplicity, cost effectiveness, low risk of contamination and the ease in establishment of conditions for nested-PCR. The method has been successfully applied to the detection of the genomic RNA from SARS-CoV, the detection limit of this method is comparable to that of the conventional two-tube nested PCR system. This method could be easily adapted to the detection of other targets.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Tao, Sheng Ce" sort="Tao, Sheng Ce" uniqKey="Tao S" first="Sheng-Ce" last="Tao">Sheng-Ce Tao</name>
</noRegion>
<name sortKey="Di Jiang" sort="Di Jiang" uniqKey="Di Jiang" last="Di Jiang">DI JIANG</name>
<name sortKey="Jing Cheng" sort="Jing Cheng" uniqKey="Jing Cheng" last="Jing Cheng">JING CHENG</name>
<name sortKey="Jing Cheng" sort="Jing Cheng" uniqKey="Jing Cheng" last="Jing Cheng">JING CHENG</name>
<name sortKey="Jing Cheng" sort="Jing Cheng" uniqKey="Jing Cheng" last="Jing Cheng">JING CHENG</name>
<name sortKey="Lu, Hong Li" sort="Lu, Hong Li" uniqKey="Lu H" first="Hong-Li" last="Lu">Hong-Li Lu</name>
<name sortKey="Tao, Sheng Ce" sort="Tao, Sheng Ce" uniqKey="Tao S" first="Sheng-Ce" last="Tao">Sheng-Ce Tao</name>
<name sortKey="Xing, Wan Li" sort="Xing, Wan Li" uniqKey="Xing W" first="Wan-Li" last="Xing">Wan-Li Xing</name>
<name sortKey="Xing, Wan Li" sort="Xing, Wan Li" uniqKey="Xing W" first="Wan-Li" last="Xing">Wan-Li Xing</name>
<name sortKey="Zhou, Yu Xiang" sort="Zhou, Yu Xiang" uniqKey="Zhou Y" first="Yu-Xiang" last="Zhou">Yu-Xiang Zhou</name>
<name sortKey="Zhou, Yu Xiang" sort="Zhou, Yu Xiang" uniqKey="Zhou Y" first="Yu-Xiang" last="Zhou">Yu-Xiang Zhou</name>
<name sortKey="Zhou, Yu Xiang" sort="Zhou, Yu Xiang" uniqKey="Zhou Y" first="Yu-Xiang" last="Zhou">Yu-Xiang Zhou</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005A97 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005A97 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:04-0257195
   |texte=   One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus

One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.