Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid
Identifieur interne : 005B75 ( Main/Exploration ); précédent : 005B74; suivant : 005B76Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid
Auteurs : Danny Tze Ming Leung [Hong Kong] ; Frankie Chi Hang Tam [Hong Kong] ; CHUN HUNG MA [Hong Kong] ; Paul Kay Sheung Chan [Hong Kong] ; Jo Lai Ken Cheung [Hong Kong] ; HAITAO NIU [Hong Kong] ; John Siu Lun Tam [Hong Kong] ; PAK LEONG LIM [Hong Kong]Source :
- The Journal of infectious diseases [ 0022-1899 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
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Abstract
The recent outbreak of severe acute respiratory syndrome (SARS) provided an opportunity to study the antibody response of infected individuals to the causative virus, SARS coronavirus. We examined serum samples obtained from 46 patients with SARS, 40 patients with non-SARS pneumonia, and 38 healthy individuals, by use of Western blotting (WB), enzyme-linked immunoassay (ELISA), and immunofluorescence assay, using both native and bacterially produced antigens of the virus. We found a highly restricted, immunoglobulin G-dominated antibody response in patients with SARS, directed most frequently (89% by ELISA) and predominantly at the nucleocapsid. Almost all of the subjects without SARS had no antinucleocapsid antibodies. The spike protein was the next most frequently targeted, but only 63% of the patients (by ELISA) responded. Other targets of the response identified by use of WB included antigens of 80 and 60 kDa. Several nonstructural proteins cloned were not antigenic, and the culture-derived nucleocapsid appeared to be specifically degraded.
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chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Chi Hang Tam, Frankie" sort="Chi Hang Tam, Frankie" uniqKey="Chi Hang Tam F" first="Frankie" last="Chi Hang Tam">Frankie Chi Hang Tam</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Chun Hung Ma" sort="Chun Hung Ma" uniqKey="Chun Hung Ma" last="Chun Hung Ma">CHUN HUNG MA</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Kay Sheung Chan, Paul" sort="Kay Sheung Chan, Paul" uniqKey="Kay Sheung Chan P" first="Paul" last="Kay Sheung Chan">Paul Kay Sheung Chan</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Lai Ken Cheung, Jo" sort="Lai Ken Cheung, Jo" uniqKey="Lai Ken Cheung J" first="Jo" last="Lai Ken Cheung">Jo Lai Ken Cheung</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Haitao Niu" sort="Haitao Niu" uniqKey="Haitao Niu" last="Haitao Niu">HAITAO NIU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Siu Lun Tam, John" sort="Siu Lun Tam, John" uniqKey="Siu Lun Tam J" first="John" last="Siu Lun Tam">John Siu Lun Tam</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Pak Leong Lim" sort="Pak Leong Lim" uniqKey="Pak Leong Lim" last="Pak Leong Lim">PAK LEONG LIM</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Human</term><term>Humoral immunity</term><term>Infection</term><term>Microbiology</term><term>Nucleocapsid</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Homme</term><term>Immunité humorale</term><term>Nucléocapside</term><term>Microbiologie</term><term>Infection</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The recent outbreak of severe acute respiratory syndrome (SARS) provided an opportunity to study the antibody response of infected individuals to the causative virus, SARS coronavirus. We examined serum samples obtained from 46 patients with SARS, 40 patients with non-SARS pneumonia, and 38 healthy individuals, by use of Western blotting (WB), enzyme-linked immunoassay (ELISA), and immunofluorescence assay, using both native and bacterially produced antigens of the virus. We found a highly restricted, immunoglobulin G-dominated antibody response in patients with SARS, directed most frequently (89% by ELISA) and predominantly at the nucleocapsid. Almost all of the subjects without SARS had no antinucleocapsid antibodies. The spike protein was the next most frequently targeted, but only 63% of the patients (by ELISA) responded. Other targets of the response identified by use of WB included antigens of 80 and 60 kDa. Several nonstructural proteins cloned were not antigenic, and the culture-derived nucleocapsid appeared to be specifically degraded.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><country name="Hong Kong"><noRegion><name sortKey="Tze Ming Leung, Danny" sort="Tze Ming Leung, Danny" uniqKey="Tze Ming Leung D" first="Danny" last="Tze Ming Leung">Danny Tze Ming Leung</name></noRegion><name sortKey="Chi Hang Tam, Frankie" sort="Chi Hang Tam, Frankie" uniqKey="Chi Hang Tam F" first="Frankie" last="Chi Hang Tam">Frankie Chi Hang Tam</name><name sortKey="Chun Hung Ma" sort="Chun Hung Ma" uniqKey="Chun Hung Ma" last="Chun Hung Ma">CHUN HUNG MA</name><name sortKey="Haitao Niu" sort="Haitao Niu" uniqKey="Haitao Niu" last="Haitao Niu">HAITAO NIU</name><name sortKey="Kay Sheung Chan, Paul" sort="Kay Sheung Chan, Paul" uniqKey="Kay Sheung Chan P" first="Paul" last="Kay Sheung Chan">Paul Kay Sheung Chan</name><name sortKey="Lai Ken Cheung, Jo" sort="Lai Ken Cheung, Jo" uniqKey="Lai Ken Cheung J" first="Jo" last="Lai Ken Cheung">Jo Lai Ken Cheung</name><name sortKey="Pak Leong Lim" sort="Pak Leong Lim" uniqKey="Pak Leong Lim" last="Pak Leong Lim">PAK LEONG LIM</name><name sortKey="Siu Lun Tam, John" sort="Siu Lun Tam, John" uniqKey="Siu Lun Tam J" first="John" last="Siu Lun Tam">John Siu Lun Tam</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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