siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Identifieur interne : 003244 ( Main/Exploration ); précédent : 003243; suivant : 003245siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Auteurs : C.-Y. Lu [Taïwan] ; H.-Y. Huang [Taïwan] ; T.-H. Yang [Taïwan] ; L.-Y. Chang [Taïwan] ; C.-Y. Lee [Taïwan] ; L.-M. Huang [Taïwan]Source :
- European Journal of Clinical Microbiology & Infectious Diseases [ 0934-9723 ] ; 2008-08-01.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Peptidyl-Dipeptidase A.
- métabolisme : Peptidyl-Dipeptidase A.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- Animaux, Cellules Vero, Chats, Extinction de l'expression des gènes, Lignée cellulaire, Réplication virale, Virus du SRAS.
- Pascal (Inist)
English descriptors
- KwdEn :
- Angiotensin, Animals, Cats, Cell Line, Chlorocebus aethiops, Coronavirus, Gene Silencing (drug effects), Microbiology, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), RNA, Small Interfering (pharmacology), Replication, SARS Virus (drug effects), SARS Virus (physiology), Severe acute respiratory syndrome, Vero Cells, Virus Replication (drug effects).
- MESH :
- chemical , genetics : Peptidyl-Dipeptidase A.
- drug effects : Gene Silencing, SARS Virus, Virus Replication.
- chemical , metabolism : Peptidyl-Dipeptidase A.
- chemical , pharmacology : RNA, Small Interfering.
- physiology : SARS Virus.
- Animals, Cats, Cell Line, Chlorocebus aethiops, Vero Cells.
Abstract
Abstract: The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.
Url:
- https://api.istex.fr/ark:/67375/VQC-KNR1MLT8-0/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088151
DOI: 10.1007/s10096-008-0495-5
Affiliations:
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Le document en format XML
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Lu</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation></author><author><name sortKey="Huang, H Y" sort="Huang, H Y" uniqKey="Huang H" first="H.-Y." last="Huang">H.-Y. Huang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yang, T H" sort="Yang, T H" uniqKey="Yang T" first="T.-H." last="Yang">T.-H. Yang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, L Y" sort="Chang, L Y" uniqKey="Chang L" first="L.-Y." last="Chang">L.-Y. Chang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lee, C Y" sort="Lee, C Y" uniqKey="Lee C" first="C.-Y." last="Lee">C.-Y. Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Huang, L M" sort="Huang, L M" uniqKey="Huang L" first="L.-M." last="Huang">L.-M. Huang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">European Journal of Clinical Microbiology & Infectious Diseases</title><title level="j" type="abbrev">Eur J Clin Microbiol Infect Dis</title><idno type="ISSN">0934-9723</idno><idno type="eISSN">1435-4373</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2008-08-01">2008-08-01</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="709">709</biblScope><biblScope unit="page" to="715">715</biblScope></imprint><idno type="ISSN">0934-9723</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0934-9723</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Angiotensin</term><term>Animals</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Gene Silencing (drug effects)</term><term>Microbiology</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>RNA, Small Interfering (pharmacology)</term><term>Replication</term><term>SARS Virus (drug effects)</term><term>SARS Virus (physiology)</term><term>Severe acute respiratory syndrome</term><term>Vero Cells</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chats</term><term>Extinction de l'expression des gènes ()</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Petit ARN interférent (pharmacologie)</term><term>Réplication virale ()</term><term>Virus du SRAS ()</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Silencing</term><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Petit ARN interférent</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chats</term><term>Extinction de l'expression des gènes</term><term>Lignée cellulaire</term><term>Réplication virale</term><term>Virus du SRAS</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Angiotensine</term><term>Cellule Vero</term><term>Coronavirus</term><term>Microbiologie</term><term>Réplication</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Lu, C Y" sort="Lu, C Y" uniqKey="Lu C" first="C.-Y." last="Lu">C.-Y. Lu</name></noRegion><name sortKey="Chang, L Y" sort="Chang, L Y" uniqKey="Chang L" first="L.-Y." last="Chang">L.-Y. Chang</name><name sortKey="Huang, H Y" sort="Huang, H Y" uniqKey="Huang H" first="H.-Y." last="Huang">H.-Y. Huang</name><name sortKey="Huang, L M" sort="Huang, L M" uniqKey="Huang L" first="L.-M." last="Huang">L.-M. Huang</name><name sortKey="Lee, C Y" sort="Lee, C Y" uniqKey="Lee C" first="C.-Y." last="Lee">C.-Y. Lee</name><name sortKey="Lu, C Y" sort="Lu, C Y" uniqKey="Lu C" first="C.-Y." last="Lu">C.-Y. Lu</name><name sortKey="Yang, T H" sort="Yang, T H" uniqKey="Yang T" first="T.-H." last="Yang">T.-H. Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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