A continuous epitope from transmissible gastroenteritis virus S protein fused to E. coli heat-labile toxin B subunit expressed by attenuated Salmonella induces serum and secretory immunity.
Identifieur interne : 006534 ( Main/Exploration ); précédent : 006533; suivant : 006535A continuous epitope from transmissible gastroenteritis virus S protein fused to E. coli heat-labile toxin B subunit expressed by attenuated Salmonella induces serum and secretory immunity.
Auteurs : C. Smerdou [Espagne] ; I M Anton ; J. Plana ; R. Curtiss ; L. EnjuanesSource :
- Virus research [ 0168-1702 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN viral, Animaux, Anticorps antiviraux (immunologie), Données de séquences moléculaires, Déterminants antigéniques des lymphocytes B (génétique), Déterminants antigéniques des lymphocytes B (immunologie), Entérotoxines (génétique), Entérotoxines (immunologie), Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Lapins, Protéines Escherichia coli, Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Salmonella typhimurium, Suidae, Séquence d'acides aminés, Séquence nucléotidique, Tests de neutralisation, Toxines bactériennes (génétique), Toxines bactériennes (immunologie), Virus de la gastroentérite transmissible (génétique), Virus de la gastroentérite transmissible (immunologie).
- MESH :
- génétique : Déterminants antigéniques des lymphocytes B, Entérotoxines, Glycoprotéines membranaires, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Toxines bactériennes, Virus de la gastroentérite transmissible.
- immunologie : Anticorps antiviraux, Déterminants antigéniques des lymphocytes B, Entérotoxines, Glycoprotéines membranaires, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Toxines bactériennes, Virus de la gastroentérite transmissible.
- ADN viral, Animaux, Données de séquences moléculaires, Expression des gènes, Glycoprotéine de spicule des coronavirus, Lapins, Protéines Escherichia coli, Salmonella typhimurium, Suidae, Séquence d'acides aminés, Séquence nucléotidique, Tests de neutralisation.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (immunology), Bacterial Toxins (genetics), Bacterial Toxins (immunology), Base Sequence, DNA, Viral, Enterotoxins (genetics), Enterotoxins (immunology), Epitopes, B-Lymphocyte (genetics), Epitopes, B-Lymphocyte (immunology), Escherichia coli Proteins, Gene Expression, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Molecular Sequence Data, Neutralization Tests, Rabbits, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Salmonella typhimurium, Spike Glycoprotein, Coronavirus, Swine, Transmissible gastroenteritis virus (genetics), Transmissible gastroenteritis virus (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Bacterial Toxins, Enterotoxins, Epitopes, B-Lymphocyte, Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Bacterial Toxins, Enterotoxins, Epitopes, B-Lymphocyte, Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- genetics : Transmissible gastroenteritis virus.
- immunology : Transmissible gastroenteritis virus.
- Amino Acid Sequence, Animals, Base Sequence, DNA, Viral, Escherichia coli Proteins, Gene Expression, Molecular Sequence Data, Neutralization Tests, Rabbits, Salmonella typhimurium, Spike Glycoprotein, Coronavirus, Swine.
Abstract
Antigenic site D from the spike protein of transmissible gastroenteritis virus (TGEV), which is a continuous epitope critical in neutralization, has been expressed as a fusion protein with E. coli heat-labile toxin B subunit (LT-B) in attenuated S. typhimurium. Synthetic peptides containing the sequence of site D induced TGEV neutralizing antibodies when inoculated subcutaneously in both rabbits and swine. A synthetic oligonucleotide encoding residues 373-398 of TGEV S protein, including antigenic site D, was cloned in frame with the 3' end of LT-B gene, into a plasmid used to transform S. typhimurium delta asd chi 3730. A collection of 6 recombinant plasmids designated pYALTB-D I-VI encoding LTB-site D fusions with a variable number of site D sequences were selected. Four of the 6 LTB-site D fusion products expressed in S. typhimurium chi 3730 formed oligomers (pentamers) that dissociated at > 70 degrees. S. typhimurium chi 3730 (pYALTB-D) V and VI expressed the oligomer forming products with higher antigenicity. Partially purified LTB-site D fusion product expressed from S. typhimurium chi 3730 (pYALTB-D) V induced anti-TGEV neutralizing antibodies in rabbits. Recombinant vaccine strain S. typhimurium delta cya delta crp delta asd chi 3987 transformed with plasmid pYALTB-D V expressed constitutively products that formed oligomers presumably containing 20 copies of site D, and showed a high stability in vitro. This recombinant strain was orally inoculated in rabbits and induced TGEV specific antibodies in both serum and intestinal secretion.
DOI: 10.1016/0168-1702(95)01265-6
PubMed: 8725098
Affiliations:
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Le document en format XML
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Smerdou</name><affiliation wicri:level="3"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Anton, I M" sort="Anton, I M" uniqKey="Anton I" first="I M" last="Anton">I M Anton</name></author><author><name sortKey="Plana, J" sort="Plana, J" uniqKey="Plana J" first="J" last="Plana">J. Plana</name></author><author><name sortKey="Curtiss, R" sort="Curtiss, R" uniqKey="Curtiss R" first="R" last="Curtiss">R. Curtiss</name></author><author><name sortKey="Enjuanes, L" sort="Enjuanes, L" uniqKey="Enjuanes L" first="L" last="Enjuanes">L. 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Smerdou</name><affiliation wicri:level="3"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Anton, I M" sort="Anton, I M" uniqKey="Anton I" first="I M" last="Anton">I M Anton</name></author><author><name sortKey="Plana, J" sort="Plana, J" uniqKey="Plana J" first="J" last="Plana">J. Plana</name></author><author><name sortKey="Curtiss, R" sort="Curtiss, R" uniqKey="Curtiss R" first="R" last="Curtiss">R. Curtiss</name></author><author><name sortKey="Enjuanes, L" sort="Enjuanes, L" uniqKey="Enjuanes L" first="L" last="Enjuanes">L. Enjuanes</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Bacterial Toxins (genetics)</term><term>Bacterial Toxins (immunology)</term><term>Base Sequence</term><term>DNA, Viral</term><term>Enterotoxins (genetics)</term><term>Enterotoxins (immunology)</term><term>Epitopes, B-Lymphocyte (genetics)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Escherichia coli Proteins</term><term>Gene Expression</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Rabbits</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (immunology)</term><term>Salmonella typhimurium</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine</term><term>Transmissible gastroenteritis virus (genetics)</term><term>Transmissible gastroenteritis virus (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral</term><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes B (génétique)</term><term>Déterminants antigéniques des lymphocytes B (immunologie)</term><term>Entérotoxines (génétique)</term><term>Entérotoxines (immunologie)</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Lapins</term><term>Protéines Escherichia coli</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Salmonella typhimurium</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tests de neutralisation</term><term>Toxines bactériennes (génétique)</term><term>Toxines bactériennes (immunologie)</term><term>Virus de la gastroentérite transmissible (génétique)</term><term>Virus de la gastroentérite transmissible (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Toxins</term><term>Enterotoxins</term><term>Epitopes, B-Lymphocyte</term><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Bacterial Toxins</term><term>Enterotoxins</term><term>Epitopes, B-Lymphocyte</term><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes B</term><term>Entérotoxines</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term><term>Toxines bactériennes</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Déterminants antigéniques des lymphocytes B</term><term>Entérotoxines</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term><term>Toxines bactériennes</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>DNA, Viral</term><term>Escherichia coli Proteins</term><term>Gene Expression</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Rabbits</term><term>Salmonella typhimurium</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Lapins</term><term>Protéines Escherichia coli</term><term>Salmonella typhimurium</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antigenic site D from the spike protein of transmissible gastroenteritis virus (TGEV), which is a continuous epitope critical in neutralization, has been expressed as a fusion protein with E. coli heat-labile toxin B subunit (LT-B) in attenuated S. typhimurium. Synthetic peptides containing the sequence of site D induced TGEV neutralizing antibodies when inoculated subcutaneously in both rabbits and swine. A synthetic oligonucleotide encoding residues 373-398 of TGEV S protein, including antigenic site D, was cloned in frame with the 3' end of LT-B gene, into a plasmid used to transform S. typhimurium delta asd chi 3730. A collection of 6 recombinant plasmids designated pYALTB-D I-VI encoding LTB-site D fusions with a variable number of site D sequences were selected. Four of the 6 LTB-site D fusion products expressed in S. typhimurium chi 3730 formed oligomers (pentamers) that dissociated at > 70 degrees. S. typhimurium chi 3730 (pYALTB-D) V and VI expressed the oligomer forming products with higher antigenicity. Partially purified LTB-site D fusion product expressed from S. typhimurium chi 3730 (pYALTB-D) V induced anti-TGEV neutralizing antibodies in rabbits. Recombinant vaccine strain S. typhimurium delta cya delta crp delta asd chi 3987 transformed with plasmid pYALTB-D V expressed constitutively products that formed oligomers presumably containing 20 copies of site D, and showed a high stability in vitro. This recombinant strain was orally inoculated in rabbits and induced TGEV specific antibodies in both serum and intestinal secretion.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><noCountry><name sortKey="Anton, I M" sort="Anton, I M" uniqKey="Anton I" first="I M" last="Anton">I M Anton</name><name sortKey="Curtiss, R" sort="Curtiss, R" uniqKey="Curtiss R" first="R" last="Curtiss">R. Curtiss</name><name sortKey="Enjuanes, L" sort="Enjuanes, L" uniqKey="Enjuanes L" first="L" last="Enjuanes">L. Enjuanes</name><name sortKey="Plana, J" sort="Plana, J" uniqKey="Plana J" first="J" last="Plana">J. Plana</name></noCountry><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Smerdou, C" sort="Smerdou, C" uniqKey="Smerdou C" first="C" last="Smerdou">C. Smerdou</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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