Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99.
Identifieur interne : 006054 ( Main/Exploration ); précédent : 006053; suivant : 006055Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99.
Auteurs : Sang-Geon Yeo [Corée du Sud] ; Mercedes Hernandez ; Peter J. Krell ; Eva E. NagySource :
- Virus genes [ 0920-8569 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire, Analyse de séquence d'ADN, Animaux, Clonage moléculaire, Coronaviridae (génétique), Diarrhée (médecine vétérinaire), Diarrhée (virologie), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Suidae (virologie), Séquence d'acides aminés, Séquence nucléotidique.
- MESH :
- génétique : Coronaviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- médecine vétérinaire : Diarrhée.
- virologie : Diarrhée, Suidae.
- ADN complémentaire, Analyse de séquence d'ADN, Animaux, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Coronaviridae (genetics), DNA, Complementary, Diarrhea (veterinary), Diarrhea (virology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Molecular Sequence Data, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Swine (virology), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical : DNA, Complementary, Spike Glycoprotein, Coronavirus.
- genetics : Coronaviridae.
- veterinary : Diarrhea.
- virology : Diarrhea, Swine.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Sequence Analysis, DNA.
Abstract
The spike (S) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to aid in the development of genetically engineered vaccines and diagnostic reagents against PEDV. The nucleotide sequence encoding the entire S gene open reading frame (ORF) of Chinju99 was 4,152 bases long encoding 1,383 amino acids. It consisted of 1,001 adenine (24.1%), 849 cytosine (20.4%), 877 guanine (21.1%) and 1425 thymine (34.3%) residues. The Chinju99 S ORF nucleotide sequence was 94.5% homologous with that of the Br1/87 and CV777 strains, respectively. The Chinju99 S protein had 92.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained 27 potential sites for asparagine (N)-linked glycosylation and there was a stretch of highly hydrophobic residues at position 1,325-1,350.
DOI: 10.1023/a:1024443112717
PubMed: 12876452
Affiliations:
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Krell</name></author><author><name sortKey="Nagy, Eva E" sort="Nagy, Eva E" uniqKey="Nagy E" first="Eva E" last="Nagy">Eva E. 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Krell</name></author><author><name sortKey="Nagy, Eva E" sort="Nagy, Eva E" uniqKey="Nagy E" first="Eva E" last="Nagy">Eva E. Nagy</name></author></analytic><series><title level="j">Virus genes</title><idno type="ISSN">0920-8569</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Coronaviridae (genetics)</term><term>DNA, Complementary</term><term>Diarrhea (veterinary)</term><term>Diarrhea (virology)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine (virology)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Clonage moléculaire</term><term>Coronaviridae (génétique)</term><term>Diarrhée (médecine vétérinaire)</term><term>Diarrhée (virologie)</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Suidae (virologie)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronaviridae</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Diarrhée</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Diarrhea</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Diarrhée</term><term>Suidae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Diarrhea</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The spike (S) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to aid in the development of genetically engineered vaccines and diagnostic reagents against PEDV. The nucleotide sequence encoding the entire S gene open reading frame (ORF) of Chinju99 was 4,152 bases long encoding 1,383 amino acids. It consisted of 1,001 adenine (24.1%), 849 cytosine (20.4%), 877 guanine (21.1%) and 1425 thymine (34.3%) residues. The Chinju99 S ORF nucleotide sequence was 94.5% homologous with that of the Br1/87 and CV777 strains, respectively. The Chinju99 S protein had 92.8% amino acid identity with that of Br1/87 and CV777, respectively. The amino acid sequence contained 27 potential sites for asparagine (N)-linked glycosylation and there was a stretch of highly hydrophobic residues at position 1,325-1,350.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Hernandez, Mercedes" sort="Hernandez, Mercedes" uniqKey="Hernandez M" first="Mercedes" last="Hernandez">Mercedes Hernandez</name><name sortKey="Krell, Peter J" sort="Krell, Peter J" uniqKey="Krell P" first="Peter J" last="Krell">Peter J. Krell</name><name sortKey="Nagy, Eva E" sort="Nagy, Eva E" uniqKey="Nagy E" first="Eva E" last="Nagy">Eva E. Nagy</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Yeo, Sang Geon" sort="Yeo, Sang Geon" uniqKey="Yeo S" first="Sang-Geon" last="Yeo">Sang-Geon Yeo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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