SrasV1, Main, Exploration, bibRecord, 005B72

Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

Identifieur interne : 005B72 ( Main/Exploration ); précédent : 005B71; suivant : 005B73

Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

Auteurs : Stephan Günther [Allemagne] ; Marcel Asper [Allemagne] ; Christina Röser [Allemagne] ; Luciano K. S. Luna [Allemagne] ; Christian Drosten [Allemagne] ; Beate Becker-Ziaja [Allemagne] ; Peter Borowski [Allemagne] ; Huan-Ming Chen [États-Unis] ; Ramachandra S. Hosmane [États-Unis]

Source :

Antiviral research [ 0166-3542 ] ; 2004.

RBID : Pascal:04-0546108

Descripteurs français

KwdFr :
- ARN viral (), ARN viral (analyse), Antiviraux (pharmacologie), Association de médicaments, Ebolavirus (), Imidazoles (), Imidazoles (synthèse chimique), Nucléosides (), Nucléosides (synthèse chimique), Réaction de polymérisation en chaîne (), Virus de Lassa (), Virus du SRAS ().
MESH :
- analyse : ARN viral.
- pharmacologie : Antiviraux.
- synthèse chimique : Imidazoles, Nucléosides.
Pascal (Inist)
- ARN viral, Association de médicaments, Ebolavirus, Imidazoles, Nucléosides, Réaction de polymérisation en chaîne, SIDA, Application, Temps réel, Réaction chaîne polymérase, Antiviral, Virus Lassa, Virus Ebola, In vitro, Analogue nucléoside, Virus de Lassa, Virus du SRAS, Virus syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- AIDS, Antiviral, Antiviral Agents (pharmacology), Application, Drug Therapy, Combination, Ebola virus, Ebolavirus (drug effects), Imidazoles (chemical synthesis), Imidazoles (chemistry), In vitro, Lassa virus, Lassa virus (drug effects), Nucleosides (chemical synthesis), Nucleosides (chemistry), Nucleosidic analog, Polymerase Chain Reaction (methods), Polymerase chain reaction, RNA, Viral (analysis), RNA, Viral (drug effects), Real time, SARS Virus (drug effects), Severe acute respiratory syndrome virus.
MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemical synthesis : Imidazoles, Nucleosides.
- chemical , chemistry : Imidazoles, Nucleosides.
- chemical , drug effects : RNA, Viral.
- chemical , pharmacology : Antiviral Agents.
- drug effects : Ebolavirus, Lassa virus, SARS Virus.
- methods : Polymerase Chain Reaction.
- Drug Therapy, Combination.

Abstract

This report describes the application of real-time PCR for testing antivirals against highly pathogenic viruses such as Lassa virus, SARS coronavirus and Ebola virus. The test combines classical cell culture with a quantitative real-time PCR read-out. The assay for Lassa virus was validated with ribavirin, which showed an IC₅₀ of 9 μg/ml. Small-scale screening identified a class of imidazole nucleoside/nucleotide analogues with antiviral activity against Lassa virus. The analogues contained either dinitrile or diester groups at the imidazole 4,5-positions, and many of which possessed an acyclic sugar or sugar phosphonate moiety at the imidazole 1-position. The IC₅₀ values of the most active compounds ranged from 5 to 21 μg/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus.

Affiliations:

Le document en format XML

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<term>Antiviral</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Application</term>
<term>Drug Therapy, Combination</term>
<term>Ebola virus</term>
<term>Ebolavirus (drug effects)</term>
<term>Imidazoles (chemical synthesis)</term>
<term>Imidazoles (chemistry)</term>
<term>In vitro</term>
<term>Lassa virus</term>
<term>Lassa virus (drug effects)</term>
<term>Nucleosides (chemical synthesis)</term>
<term>Nucleosides (chemistry)</term>
<term>Nucleosidic analog</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Polymerase chain reaction</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (drug effects)</term>
<term>Real time</term>
<term>SARS Virus (drug effects)</term>
<term>Severe acute respiratory syndrome virus</term>
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<term>ARN viral (analyse)</term>
<term>Antiviraux (pharmacologie)</term>
<term>Association de médicaments</term>
<term>Ebolavirus ()</term>
<term>Imidazoles ()</term>
<term>Imidazoles (synthèse chimique)</term>
<term>Nucléosides ()</term>
<term>Nucléosides (synthèse chimique)</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Virus de Lassa ()</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Imidazoles</term>
<term>Nucléosides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Drug Therapy, Combination</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>ARN viral</term>
<term>Association de médicaments</term>
<term>Ebolavirus</term>
<term>Imidazoles</term>
<term>Nucléosides</term>
<term>Réaction de polymérisation en chaîne</term>
<term>SIDA</term>
<term>Application</term>
<term>Temps réel</term>
<term>Réaction chaîne polymérase</term>
<term>Antiviral</term>
<term>Virus Lassa</term>
<term>Virus Ebola</term>
<term>In vitro</term>
<term>Analogue nucléoside</term>
<term>Virus de Lassa</term>
<term>Virus du SRAS</term>
<term>Virus syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">This report describes the application of real-time PCR for testing antivirals against highly pathogenic viruses such as Lassa virus, SARS coronavirus and Ebola virus. The test combines classical cell culture with a quantitative real-time PCR read-out. The assay for Lassa virus was validated with ribavirin, which showed an IC<sub>50</sub>
 of 9 μg/ml. Small-scale screening identified a class of imidazole nucleoside/nucleotide analogues with antiviral activity against Lassa virus. The analogues contained either dinitrile or diester groups at the imidazole 4,5-positions, and many of which possessed an acyclic sugar or sugar phosphonate moiety at the imidazole 1-position. The IC<sub>50</sub>
 values of the most active compounds ranged from 5 to 21 μg/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>États-Unis</li>
</country>
<region><li>Hambourg</li>
<li>Maryland</li>
</region>
<settlement><li>College Park (Maryland)</li>
<li>Hambourg</li>
</settlement>
<orgName><li>Université du Maryland</li>
</orgName>
</list>
<tree><country name="Allemagne"><region name="Hambourg"><name sortKey="Gunther, Stephan" sort="Gunther, Stephan" uniqKey="Gunther S" first="Stephan" last="Günther">Stephan Günther</name>
</region>
<name sortKey="Asper, Marcel" sort="Asper, Marcel" uniqKey="Asper M" first="Marcel" last="Asper">Marcel Asper</name>
<name sortKey="Becker Ziaja, Beate" sort="Becker Ziaja, Beate" uniqKey="Becker Ziaja B" first="Beate" last="Becker-Ziaja">Beate Becker-Ziaja</name>
<name sortKey="Borowski, Peter" sort="Borowski, Peter" uniqKey="Borowski P" first="Peter" last="Borowski">Peter Borowski</name>
<name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<name sortKey="Luna, Luciano K S" sort="Luna, Luciano K S" uniqKey="Luna L" first="Luciano K. S." last="Luna">Luciano K. S. Luna</name>
<name sortKey="Roser, Christina" sort="Roser, Christina" uniqKey="Roser C" first="Christina" last="Röser">Christina Röser</name>
</country>
<country name="États-Unis"><region name="Maryland"><name sortKey="Chen, Huan Ming" sort="Chen, Huan Ming" uniqKey="Chen H" first="Huan-Ming" last="Chen">Huan-Ming Chen</name>
</region>
<name sortKey="Hosmane, Ramachandra S" sort="Hosmane, Ramachandra S" uniqKey="Hosmane R" first="Ramachandra S." last="Hosmane">Ramachandra S. Hosmane</name>
</country>
</tree>
</affiliations>
</record>

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Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

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