Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies
Identifieur interne : 005B28 ( Main/Exploration ); précédent : 005B27; suivant : 005B29Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies
Auteurs : HONG ZHANG [République populaire de Chine] ; GUANGWEN WANG [République populaire de Chine] ; JIAN LI [République populaire de Chine] ; YUCHUN NIE [République populaire de Chine] ; XUANLING SHI [République populaire de Chine] ; GEWEI LIAN [République populaire de Chine] ; WEI WANG [République populaire de Chine] ; XIAOLEI YIN [République populaire de Chine] ; YANG ZHAO [République populaire de Chine] ; XIUXIA QU [République populaire de Chine] ; MINGXIAO DING [République populaire de Chine] ; HONGKUI DENG [République populaire de Chine]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.
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wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Guangwen Wang" sort="Guangwen Wang" uniqKey="Guangwen Wang" last="Guangwen Wang">GUANGWEN WANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Jian Li" sort="Jian Li" uniqKey="Jian Li" last="Jian Li">JIAN LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement 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type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Xuanling Shi" sort="Xuanling Shi" uniqKey="Xuanling Shi" last="Xuanling Shi">XUANLING SHI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Gewei Lian" sort="Gewei Lian" uniqKey="Gewei Lian" last="Gewei Lian">GEWEI LIAN</name><affiliation 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type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Yang Zhao" sort="Yang Zhao" uniqKey="Yang Zhao" last="Yang Zhao">YANG ZHAO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Xiuxia Qu" sort="Xiuxia Qu" uniqKey="Xiuxia Qu" last="Xiuxia Qu">XIUXIA QU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Mingxiao Ding" sort="Mingxiao Ding" uniqKey="Mingxiao Ding" last="Mingxiao Ding">MINGXIAO DING</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term><term>Coronavirus</term><term>Glycoprotein</term><term>Identification</term><term>Microbiology</term><term>Neutralization</term><term>Neutralizing antibody</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Identification</term><term>Déterminant antigénique</term><term>Glycoprotéine</term><term>Anticorps neutralisant</term><term>Neutralisation</term><term>Microbiologie</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Hong Zhang" sort="Hong Zhang" uniqKey="Hong Zhang" last="Hong Zhang">HONG ZHANG</name></noRegion><name sortKey="Gewei Lian" sort="Gewei Lian" uniqKey="Gewei Lian" last="Gewei Lian">GEWEI LIAN</name><name sortKey="Guangwen Wang" sort="Guangwen Wang" uniqKey="Guangwen Wang" last="Guangwen Wang">GUANGWEN WANG</name><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><name sortKey="Jian Li" sort="Jian Li" uniqKey="Jian Li" last="Jian Li">JIAN LI</name><name sortKey="Mingxiao Ding" sort="Mingxiao Ding" uniqKey="Mingxiao Ding" last="Mingxiao Ding">MINGXIAO DING</name><name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name><name sortKey="Xiaolei Yin" sort="Xiaolei Yin" uniqKey="Xiaolei Yin" last="Xiaolei Yin">XIAOLEI YIN</name><name sortKey="Xiuxia Qu" sort="Xiuxia Qu" uniqKey="Xiuxia Qu" last="Xiuxia Qu">XIUXIA QU</name><name sortKey="Xuanling Shi" sort="Xuanling Shi" uniqKey="Xuanling Shi" last="Xuanling Shi">XUANLING SHI</name><name sortKey="Yang Zhao" sort="Yang Zhao" uniqKey="Yang Zhao" last="Yang Zhao">YANG ZHAO</name><name sortKey="Yuchun Nie" sort="Yuchun Nie" uniqKey="Yuchun Nie" last="Yuchun Nie">YUCHUN NIE</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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