Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.
Identifieur interne : 005346 ( Main/Exploration ); précédent : 005345; suivant : 005347Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.
Auteurs : Yue Huang [États-Unis] ; Zhi-Yong Yang ; Wing-Pui Kong ; Gary J. NabelSource :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Assemblage viral, Données de séquences moléculaires, Gènes viraux (physiologie), Humains, Nucléocapside (physiologie), Protéines de la matrice virale (génétique), Protéines nucléocapside (génétique), Séquence nucléotidique, Vaccins antiviraux (immunologie), Virion (physiologie), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines de la matrice virale, Protéines nucléocapside, Virus du SRAS.
- immunologie : Vaccins antiviraux, Virus du SRAS.
- physiologie : Gènes viraux, Nucléocapside, Virion, Virus du SRAS.
- Assemblage viral, Données de séquences moléculaires, Humains, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Genes, Viral (physiology), Humans, Molecular Sequence Data, Nucleocapsid (physiology), Nucleocapsid Proteins (genetics), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (physiology), Viral Matrix Proteins (genetics), Viral Vaccines (immunology), Virion (physiology), Virus Assembly.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Matrix Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus, Viral Vaccines.
- physiology : Genes, Viral, Nucleocapsid, SARS Virus, Virion.
- Base Sequence, Humans, Molecular Sequence Data, Virus Assembly.
Abstract
The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.
DOI: 10.1128/JVI.78.22.12557-12565.2004
PubMed: 15507643
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A77
- to stream PubMed, to step Curation: 002A77
- to stream PubMed, to step Checkpoint: 002C98
- to stream Ncbi, to step Merge: 000B66
- to stream Ncbi, to step Curation: 000B66
- to stream Ncbi, to step Checkpoint: 000B66
- to stream Main, to step Merge: 005762
- to stream Main, to step Curation: 005346
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.</title><author><name sortKey="Huang, Yue" sort="Huang, Yue" uniqKey="Huang Y" first="Yue" last="Huang">Yue Huang</name><affiliation wicri:level="2"><nlm:affiliation>Vaccine Research Center, Natiuonal Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, MD 20982-3005, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaccine Research Center, Natiuonal Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, MD 20982-3005</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name></author><author><name sortKey="Nabel, Gary J" sort="Nabel, Gary J" uniqKey="Nabel G" first="Gary J" last="Nabel">Gary J. Nabel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15507643</idno><idno type="pmid">15507643</idno><idno type="doi">10.1128/JVI.78.22.12557-12565.2004</idno><idno type="wicri:Area/PubMed/Corpus">002A77</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A77</idno><idno type="wicri:Area/PubMed/Curation">002A77</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A77</idno><idno type="wicri:Area/PubMed/Checkpoint">002C98</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C98</idno><idno type="wicri:Area/Ncbi/Merge">000B66</idno><idno type="wicri:Area/Ncbi/Curation">000B66</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B66</idno><idno type="wicri:doubleKey">0022-538X:2004:Huang Y:generation:of:synthetic</idno><idno type="wicri:Area/Main/Merge">005762</idno><idno type="wicri:Area/Main/Curation">005346</idno><idno type="wicri:Area/Main/Exploration">005346</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.</title><author><name sortKey="Huang, Yue" sort="Huang, Yue" uniqKey="Huang Y" first="Yue" last="Huang">Yue Huang</name><affiliation wicri:level="2"><nlm:affiliation>Vaccine Research Center, Natiuonal Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, MD 20982-3005, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaccine Research Center, Natiuonal Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, MD 20982-3005</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name></author><author><name sortKey="Nabel, Gary J" sort="Nabel, Gary J" uniqKey="Nabel G" first="Gary J" last="Nabel">Gary J. Nabel</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Genes, Viral (physiology)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Nucleocapsid (physiology)</term><term>Nucleocapsid Proteins (genetics)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (physiology)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Vaccines (immunology)</term><term>Virion (physiology)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Assemblage viral</term><term>Données de séquences moléculaires</term><term>Gènes viraux (physiologie)</term><term>Humains</term><term>Nucléocapside (physiologie)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines nucléocapside (génétique)</term><term>Séquence nucléotidique</term><term>Vaccins antiviraux (immunologie)</term><term>Virion (physiologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Gènes viraux</term><term>Nucléocapside</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Genes, Viral</term><term>Nucleocapsid</term><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Assemblage viral</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><name sortKey="Nabel, Gary J" sort="Nabel, Gary J" uniqKey="Nabel G" first="Gary J" last="Nabel">Gary J. Nabel</name><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Huang, Yue" sort="Huang, Yue" uniqKey="Huang Y" first="Yue" last="Huang">Yue Huang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005346 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005346 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15507643
|texte= Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15507643" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |