Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
Identifieur interne : 005173 ( Main/Exploration ); précédent : 005172; suivant : 005174Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
Auteurs : Junhui Zhai [République populaire de Chine] ; Thomas Briese [États-Unis] ; Erhei Dai [République populaire de Chine] ; Xiaoyi Wang [République populaire de Chine] ; Xin Pang [République populaire de Chine] ; Zongmin Du [République populaire de Chine] ; Haihong Liu [République populaire de Chine] ; Jin Wang [République populaire de Chine] ; Hongxia Wang [République populaire de Chine] ; Zhaobiao Guo [République populaire de Chine] ; Zeliang Chen [République populaire de Chine] ; Lingxiao Jiang [République populaire de Chine] ; Dongsheng Zhou [République populaire de Chine] ; Yanping Han [République populaire de Chine] ; Omar Jabado [États-Unis] ; Gustavo Palacios [États-Unis] ; W. Ian Lipkin [États-Unis] ; Ruifu Yang [République populaire de Chine]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN viral (génétique), ARN viral (génétique), ARN viral (isolement et purification), ARN viral (sang), Amorces ADN (génétique), Anticorps antiviraux (sang), Chine (épidémiologie), Facteurs temps, Flambées de maladies, Fèces (virologie), Humains, Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (normes), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- génétique : ADN viral, ARN viral, Amorces ADN, Virus du SRAS.
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : ARN viral, Virus du SRAS.
- normes : Réaction de polymérisation en chaîne.
- sang : ARN viral, Anticorps antiviraux.
- virologie : Fèces, Syndrome respiratoire aigu sévère.
- épidémiologie : Chine, Syndrome respiratoire aigu sévère.
- Facteurs temps, Flambées de maladies, Humains, Réaction de polymérisation en chaîne, Séquence nucléotidique.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Base Sequence, China (epidemiology), DNA Primers (genetics), DNA, Viral (genetics), Disease Outbreaks, Feces (virology), Humans, Polymerase Chain Reaction (methods), Polymerase Chain Reaction (standards), RNA, Viral (blood), RNA, Viral (genetics), RNA, Viral (isolation & purification), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Time Factors.
- MESH :
- chemical , blood : Antibodies, Viral, RNA, Viral.
- geographic , epidemiology : China.
- chemical , genetics : DNA Primers, DNA, Viral, RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- standards : Polymerase Chain Reaction.
- virology : Feces, Severe Acute Respiratory Syndrome.
- Base Sequence, Disease Outbreaks, Humans, Time Factors.
Abstract
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
Url:
DOI: 10.3201/eid1002.030799
PubMed: 15030701
PubMed Central: 3322935
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000849
- to stream Pmc, to step Curation: 000849
- to stream Pmc, to step Checkpoint: 001550
- to stream PubMed, to step Corpus: 002F03
- to stream PubMed, to step Curation: 002F03
- to stream PubMed, to step Checkpoint: 002B18
- to stream Ncbi, to step Merge: 000680
- to stream Ncbi, to step Curation: 000680
- to stream Ncbi, to step Checkpoint: 000680
- to stream Main, to step Merge: 005553
- to stream Main, to step Curation: 005173
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003</title><author><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation><nlm:aff id="aff3">Junhui Zhai and Thomas Briese contributed equally to the manuscript.</nlm:aff></affiliation></author><author><name sortKey="Briese, Thomas" sort="Briese, Thomas" uniqKey="Briese T" first="Thomas" last="Briese">Thomas Briese</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation><nlm:aff id="aff3">Junhui Zhai and Thomas Briese contributed equally to the manuscript.</nlm:aff></affiliation></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Haihong" sort="Liu, Haihong" uniqKey="Liu H" first="Haihong" last="Liu">Haihong Liu</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jiang, Lingxiao" sort="Jiang, Lingxiao" uniqKey="Jiang L" first="Lingxiao" last="Jiang">Lingxiao Jiang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Han, Yanping" sort="Han, Yanping" uniqKey="Han Y" first="Yanping" last="Han">Yanping Han</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jabado, Omar" sort="Jabado, Omar" uniqKey="Jabado O" first="Omar" last="Jabado">Omar Jabado</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Palacios, Gustavo" sort="Palacios, Gustavo" uniqKey="Palacios G" first="Gustavo" last="Palacios">Gustavo Palacios</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Lipkin, W Ian" sort="Lipkin, W Ian" uniqKey="Lipkin W" first="W. Ian" last="Lipkin">W. Ian Lipkin</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15030701</idno><idno type="pmc">3322935</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322935</idno><idno type="RBID">PMC:3322935</idno><idno type="doi">10.3201/eid1002.030799</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000849</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000849</idno><idno type="wicri:Area/Pmc/Curation">000849</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000849</idno><idno type="wicri:Area/Pmc/Checkpoint">001550</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001550</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15030701</idno><idno type="wicri:Area/PubMed/Corpus">002F03</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F03</idno><idno type="wicri:Area/PubMed/Curation">002F03</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F03</idno><idno type="wicri:Area/PubMed/Checkpoint">002B18</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B18</idno><idno type="wicri:Area/Ncbi/Merge">000680</idno><idno type="wicri:Area/Ncbi/Curation">000680</idno><idno type="wicri:Area/Ncbi/Checkpoint">000680</idno><idno type="wicri:doubleKey">1080-6040:2004:Zhai J:real:time:polymerase</idno><idno type="wicri:Area/Main/Merge">005553</idno><idno type="wicri:Area/Main/Curation">005173</idno><idno type="wicri:Area/Main/Exploration">005173</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003</title><author><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation><nlm:aff id="aff3">Junhui Zhai and Thomas Briese contributed equally to the manuscript.</nlm:aff></affiliation></author><author><name sortKey="Briese, Thomas" sort="Briese, Thomas" uniqKey="Briese T" first="Thomas" last="Briese">Thomas Briese</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation><nlm:aff id="aff3">Junhui Zhai and Thomas Briese contributed equally to the manuscript.</nlm:aff></affiliation></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Haihong" sort="Liu, Haihong" uniqKey="Liu H" first="Haihong" last="Liu">Haihong Liu</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jiang, Lingxiao" sort="Jiang, Lingxiao" uniqKey="Jiang L" first="Lingxiao" last="Jiang">Lingxiao Jiang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Han, Yanping" sort="Han, Yanping" uniqKey="Han Y" first="Yanping" last="Han">Yanping Han</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jabado, Omar" sort="Jabado, Omar" uniqKey="Jabado O" first="Omar" last="Jabado">Omar Jabado</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Palacios, Gustavo" sort="Palacios, Gustavo" uniqKey="Palacios G" first="Gustavo" last="Palacios">Gustavo Palacios</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Lipkin, W Ian" sort="Lipkin, W Ian" uniqKey="Lipkin W" first="W. Ian" last="Lipkin">W. Ian Lipkin</name><affiliation wicri:level="2"><nlm:aff id="aff2">Mailman School of Public Health of Columbia University, New York, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mailman School of Public Health of Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1">Institute of Microbiology and Epidemiology, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Base Sequence</term><term>China (epidemiology)</term><term>DNA Primers (genetics)</term><term>DNA, Viral (genetics)</term><term>Disease Outbreaks</term><term>Feces (virology)</term><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (standards)</term><term>RNA, Viral (blood)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (isolation & purification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>ARN viral (génétique)</term><term>ARN viral (isolement et purification)</term><term>ARN viral (sang)</term><term>Amorces ADN (génétique)</term><term>Anticorps antiviraux (sang)</term><term>Chine (épidémiologie)</term><term>Facteurs temps</term><term>Flambées de maladies</term><term>Fèces (virologie)</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (normes)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Séquence nucléotidique</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Amorces ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN viral</term><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Disease Outbreaks</term><term>Humans</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Flambées de maladies</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name></noRegion><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name><name sortKey="Han, Yanping" sort="Han, Yanping" uniqKey="Han Y" first="Yanping" last="Han">Yanping Han</name><name sortKey="Jiang, Lingxiao" sort="Jiang, Lingxiao" uniqKey="Jiang L" first="Lingxiao" last="Jiang">Lingxiao Jiang</name><name sortKey="Liu, Haihong" sort="Liu, Haihong" uniqKey="Liu H" first="Haihong" last="Liu">Haihong Liu</name><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Briese, Thomas" sort="Briese, Thomas" uniqKey="Briese T" first="Thomas" last="Briese">Thomas Briese</name></region><name sortKey="Jabado, Omar" sort="Jabado, Omar" uniqKey="Jabado O" first="Omar" last="Jabado">Omar Jabado</name><name sortKey="Lipkin, W Ian" sort="Lipkin, W Ian" uniqKey="Lipkin W" first="W. Ian" last="Lipkin">W. Ian Lipkin</name><name sortKey="Palacios, Gustavo" sort="Palacios, Gustavo" uniqKey="Palacios G" first="Gustavo" last="Palacios">Gustavo Palacios</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005173 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005173 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3322935
|texte= Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15030701" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |