Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein.
Identifieur interne : 005051 ( Main/Exploration ); précédent : 005050; suivant : 005052Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein.
Auteurs : Qiulong Huang [États-Unis] ; Liping Yu ; Andrew M. Petros ; Angelo Gunasekera ; Zhihong Liu ; Nan Xu ; Philip Hajduk ; Jamey Mack ; Stephen W. Fesik ; Edward T. OlejniczakSource :
- Biochemistry [ 0006-2960 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , metabolism : RNA.
- chemical : Ligands.
- chemistry : SARS Virus.
- Animals, Binding Sites, Humans, Models, Molecular, Molecular Sequence Data, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Protein Folding, Protein Structure, Tertiary.
Abstract
The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.
DOI: 10.1021/bi036155b
PubMed: 15147189
Affiliations:
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Le document en format XML
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<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Molecular Structure</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Protein Folding</term>
<term>Protein Structure, Tertiary</term>
<term>RNA (metabolism)</term>
<term>SARS Virus (chemistry)</term>
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<term>Ligands</term>
<term>Modèles moléculaires</term>
<term>Pliage des protéines</term>
<term>Protéines nucléocapside ()</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
<term>Sites de fixation</term>
<term>Structure moléculaire</term>
<term>Structure tertiaire des protéines</term>
<term>Virus du SRAS ()</term>
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<term>Binding Sites</term>
<term>Humans</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Molecular Structure</term>
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<term>Structure tertiaire des protéines</term>
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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.</div>
</front>
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<tree><noCountry><name sortKey="Fesik, Stephen W" sort="Fesik, Stephen W" uniqKey="Fesik S" first="Stephen W" last="Fesik">Stephen W. Fesik</name>
<name sortKey="Gunasekera, Angelo" sort="Gunasekera, Angelo" uniqKey="Gunasekera A" first="Angelo" last="Gunasekera">Angelo Gunasekera</name>
<name sortKey="Hajduk, Philip" sort="Hajduk, Philip" uniqKey="Hajduk P" first="Philip" last="Hajduk">Philip Hajduk</name>
<name sortKey="Liu, Zhihong" sort="Liu, Zhihong" uniqKey="Liu Z" first="Zhihong" last="Liu">Zhihong Liu</name>
<name sortKey="Mack, Jamey" sort="Mack, Jamey" uniqKey="Mack J" first="Jamey" last="Mack">Jamey Mack</name>
<name sortKey="Olejniczak, Edward T" sort="Olejniczak, Edward T" uniqKey="Olejniczak E" first="Edward T" last="Olejniczak">Edward T. Olejniczak</name>
<name sortKey="Petros, Andrew M" sort="Petros, Andrew M" uniqKey="Petros A" first="Andrew M" last="Petros">Andrew M. Petros</name>
<name sortKey="Xu, Nan" sort="Xu, Nan" uniqKey="Xu N" first="Nan" last="Xu">Nan Xu</name>
<name sortKey="Yu, Liping" sort="Yu, Liping" uniqKey="Yu L" first="Liping" last="Yu">Liping Yu</name>
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<country name="États-Unis"><noRegion><name sortKey="Huang, Qiulong" sort="Huang, Qiulong" uniqKey="Huang Q" first="Qiulong" last="Huang">Qiulong Huang</name>
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