SrasV1, Main, Exploration, bibRecord, 004E28

Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication

Identifieur interne : 004E28 ( Main/Exploration ); précédent : 004E27; suivant : 004E29

Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication

Auteurs : Eric C. Mossel [États-Unis] ; CHENG HUANG [États-Unis] ; Krishna Narayanan [États-Unis] ; Shinji Makino [États-Unis] ; Robert B. Tesh [États-Unis] ; C. J. Peters [États-Unis]

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0159803

Descripteurs français

KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (physiologie), Cellules Caco-2, Cellules Vero, Chats, Cricetinae, Herpestidae, Humains, Lapins, Lignée cellulaire, Peptidyl-Dipeptidase A, Récepteurs viraux (génétique), Récepteurs viraux (physiologie), Réplication virale, Souris, Suidae, Tupaia, Virus du SRAS (croissance et développement), Virus du SRAS (physiologie), Visons.
MESH :
- croissance et développement : Virus du SRAS.
- génétique : Carboxypeptidases, Récepteurs viraux.
- physiologie : Carboxypeptidases, Récepteurs viraux, Virus du SRAS.
Pascal (Inist)
- Animaux, Cellules Caco-2, Cellules Vero, Chats, Coronavirus, Cricetinae, Herpestidae, Humains, Lapins, Lignée cellulaire, Peptidyl-Dipeptidase A, Réplication, Microbiologie, Réplication virale, Souris, Suidae, Tupaia, Virologie, Syndrome respiratoire aigu sévère, Visons.

English descriptors

KwdEn :
- Animals, Caco-2 Cells, Carboxypeptidases (genetics), Carboxypeptidases (physiology), Cats, Cell Line, Cell line, Chlorocebus aethiops, Coronavirus, Cricetinae, Herpestidae, Humans, Mice, Microbiology, Mink, Peptidyl-Dipeptidase A, Rabbits, Receptors, Virus (genetics), Receptors, Virus (physiology), Replication, SARS Virus (growth & development), SARS Virus (physiology), Severe acute respiratory syndrome, Swine, Tupaia, Vero Cells, Virology, Virus Replication.
MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , physiology : Carboxypeptidases, Receptors, Virus.
- growth & development : SARS Virus.
- physiology : SARS Virus.
- Animals, Caco-2 Cells, Cats, Cell Line, Chlorocebus aethiops, Cricetinae, Herpestidae, Humans, Mice, Mink, Peptidyl-Dipeptidase A, Rabbits, Swine, Tupaia, Vero Cells, Virus Replication.

Abstract

Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Caco-2 Cells</term>
<term>Carboxypeptidases (genetics)</term>
<term>Carboxypeptidases (physiology)</term>
<term>Cats</term>
<term>Cell Line</term>
<term>Cell line</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus</term>
<term>Cricetinae</term>
<term>Herpestidae</term>
<term>Humans</term>
<term>Mice</term>
<term>Microbiology</term>
<term>Mink</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Rabbits</term>
<term>Receptors, Virus (genetics)</term>
<term>Receptors, Virus (physiology)</term>
<term>Replication</term>
<term>SARS Virus (growth & development)</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Swine</term>
<term>Tupaia</term>
<term>Vero Cells</term>
<term>Virology</term>
<term>Virus Replication</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Carboxypeptidases (génétique)</term>
<term>Carboxypeptidases (physiologie)</term>
<term>Cellules Caco-2</term>
<term>Cellules Vero</term>
<term>Chats</term>
<term>Cricetinae</term>
<term>Herpestidae</term>
<term>Humains</term>
<term>Lapins</term>
<term>Lignée cellulaire</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Récepteurs viraux (génétique)</term>
<term>Récepteurs viraux (physiologie)</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Suidae</term>
<term>Tupaia</term>
<term>Virus du SRAS (croissance et développement)</term>
<term>Virus du SRAS (physiologie)</term>
<term>Visons</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term>
<term>Receptors, Virus</term>
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<term>Receptors, Virus</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxypeptidases</term>
<term>Récepteurs viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Carboxypeptidases</term>
<term>Récepteurs viraux</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Caco-2 Cells</term>
<term>Cats</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Cricetinae</term>
<term>Herpestidae</term>
<term>Humans</term>
<term>Mice</term>
<term>Mink</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Rabbits</term>
<term>Swine</term>
<term>Tupaia</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Cellules Caco-2</term>
<term>Cellules Vero</term>
<term>Chats</term>
<term>Coronavirus</term>
<term>Cricetinae</term>
<term>Herpestidae</term>
<term>Humains</term>
<term>Lapins</term>
<term>Lignée cellulaire</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Réplication</term>
<term>Microbiologie</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Suidae</term>
<term>Tupaia</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Visons</term>
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<front><div type="abstract" xml:lang="en">Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
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<tree><country name="États-Unis"><region name="Texas"><name sortKey="Mossel, Eric C" sort="Mossel, Eric C" uniqKey="Mossel E" first="Eric C." last="Mossel">Eric C. Mossel</name>
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<name sortKey="Cheng Huang" sort="Cheng Huang" uniqKey="Cheng Huang" last="Cheng Huang">CHENG HUANG</name>
<name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
<name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
<name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C. J." last="Peters">C. J. Peters</name>
<name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C. J." last="Peters">C. J. Peters</name>
<name sortKey="Tesh, Robert B" sort="Tesh, Robert B" uniqKey="Tesh R" first="Robert B." last="Tesh">Robert B. Tesh</name>
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Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication

Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication

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Descripteurs français

English descriptors

Abstract

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Le document en format XML

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Pour mettre un lien sur cette page dans le réseau Wicri

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