Stem-loop IV in the 5' untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication
Identifieur interne : 004D59 ( Main/Exploration ); précédent : 004D58; suivant : 004D60Stem-loop IV in the 5' untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication
Auteurs : Sharmila Raman [États-Unis] ; David A. Brian [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (biosynthèse), ARN viral (génétique), Conformation d'acide nucléique, Coronavirus bovin (), Coronavirus bovin (génétique), Coronavirus bovin (physiologie), Données de séquences moléculaires, Humains, Liaison aux protéines, Lignée cellulaire, Masse moléculaire, Protéines (métabolisme), Régions 5' non traduites, Réplication virale, Séquence nucléotidique, Test de retard de migration électrophorétique, Virus du SRAS (génétique), Virus défectifs (génétique), Virus défectifs (physiologie).
- MESH :
- biosynthèse : ARN viral.
- génétique : ARN viral, Coronavirus bovin, Virus du SRAS, Virus défectifs.
- métabolisme : Protéines.
- physiologie : Coronavirus bovin, Virus défectifs.
- Pascal (Inist)
- ARN viral, Conformation d'acide nucléique, Coronavirus bovin, Données de séquences moléculaires, Elément IS, Humains, Liaison aux protéines, Lignée cellulaire, Masse moléculaire, Régions 5' non traduites, Réplication, Microbiologie, Réplication virale, Séquence nucléotidique, Test de retard de migration électrophorétique, Virologie.
English descriptors
- KwdEn :
- 5' Untranslated Regions, Base Sequence, Bovine coronavirus, Cell Line, Coronavirus, Bovine (chemistry), Coronavirus, Bovine (genetics), Coronavirus, Bovine (physiology), Defective Viruses (genetics), Defective Viruses (physiology), Electrophoretic Mobility Shift Assay, Humans, IS element, Microbiology, Molecular Sequence Data, Molecular Weight, Nucleic Acid Conformation, Protein Binding, Proteins (metabolism), RNA, Viral (biosynthesis), RNA, Viral (chemistry), RNA, Viral (genetics), Replication, SARS Virus (genetics), Virology, Virus Replication.
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , chemistry : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical , metabolism : Proteins.
- chemical : 5' Untranslated Regions.
- chemistry : Coronavirus, Bovine.
- genetics : Coronavirus, Bovine, Defective Viruses, SARS Virus.
- physiology : Coronavirus, Bovine, Defective Viruses.
- Base Sequence, Cell Line, Electrophoretic Mobility Shift Assay, Humans, Molecular Sequence Data, Molecular Weight, Nucleic Acid Conformation, Protein Binding, Virus Replication.
Abstract
The 210-nucleotide (nt) 5' untranslated region (UTR) in the positive-strand bovine coronavirus (BCoV) genome is predicted to contain four higher-order structures identified as stem-loops I to IV, which may function as cis-acting elements in genomic RNA replication. Here, we describe evidence that stem-loop IV, a bulged stem-loop mapping at nt 186 through 215, (i) is phylogenetically conserved among group 2 coronaviruses and may have a homolog in groups 1 and 3, (ii) exists as a higher-order structure on the basis of enzyme probing, (iii) is required as a higher-order element for replication of a BCoV defective interfering (DI) RNA in the positive but not the negative strand, and (iv) as a higher-order structure in wild-type (wt) and mutant molecules that replicate, specifically binds six cellular proteins in the molecular mass range of 25 to 58 kDa as determined by electrophoretic mobility shift and UV cross-linking assays; binding to viral proteins was not detected. Interestingly, the predicted stem-loop IV homolog in the severe acute respiratory syndrome (SARS) coronavirus appears to be group 1-like in that it is in part duplicated with a group 1-like conserved loop sequence and is not group 2-like, as would be expected by the SARS coronavirus group 2-like 3' UTR structure. These results together indicate that stem-loop IV in the BCoV 5' UTR is a cis-acting element for DI RNA replication and that it might function through interactions with cellular proteins. It is postulated that stem-loop IV functions similarly in the virus genome.
Affiliations:
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Le document en format XML
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<term>Bovine coronavirus</term>
<term>Cell Line</term>
<term>Coronavirus, Bovine (chemistry)</term>
<term>Coronavirus, Bovine (genetics)</term>
<term>Coronavirus, Bovine (physiology)</term>
<term>Defective Viruses (genetics)</term>
<term>Defective Viruses (physiology)</term>
<term>Electrophoretic Mobility Shift Assay</term>
<term>Humans</term>
<term>IS element</term>
<term>Microbiology</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
<term>Nucleic Acid Conformation</term>
<term>Protein Binding</term>
<term>Proteins (metabolism)</term>
<term>RNA, Viral (biosynthesis)</term>
<term>RNA, Viral (chemistry)</term>
<term>RNA, Viral (genetics)</term>
<term>Replication</term>
<term>SARS Virus (genetics)</term>
<term>Virology</term>
<term>Virus Replication</term>
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<term>ARN viral (biosynthèse)</term>
<term>ARN viral (génétique)</term>
<term>Conformation d'acide nucléique</term>
<term>Coronavirus bovin ()</term>
<term>Coronavirus bovin (génétique)</term>
<term>Coronavirus bovin (physiologie)</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Masse moléculaire</term>
<term>Protéines (métabolisme)</term>
<term>Régions 5' non traduites</term>
<term>Réplication virale</term>
<term>Séquence nucléotidique</term>
<term>Test de retard de migration électrophorétique</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus défectifs (génétique)</term>
<term>Virus défectifs (physiologie)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus, Bovine</term>
<term>Defective Viruses</term>
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<term>Virus défectifs</term>
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<term>Cell Line</term>
<term>Electrophoretic Mobility Shift Assay</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
<term>Nucleic Acid Conformation</term>
<term>Protein Binding</term>
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<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en">The 210-nucleotide (nt) 5' untranslated region (UTR) in the positive-strand bovine coronavirus (BCoV) genome is predicted to contain four higher-order structures identified as stem-loops I to IV, which may function as cis-acting elements in genomic RNA replication. Here, we describe evidence that stem-loop IV, a bulged stem-loop mapping at nt 186 through 215, (i) is phylogenetically conserved among group 2 coronaviruses and may have a homolog in groups 1 and 3, (ii) exists as a higher-order structure on the basis of enzyme probing, (iii) is required as a higher-order element for replication of a BCoV defective interfering (DI) RNA in the positive but not the negative strand, and (iv) as a higher-order structure in wild-type (wt) and mutant molecules that replicate, specifically binds six cellular proteins in the molecular mass range of 25 to 58 kDa as determined by electrophoretic mobility shift and UV cross-linking assays; binding to viral proteins was not detected. Interestingly, the predicted stem-loop IV homolog in the severe acute respiratory syndrome (SARS) coronavirus appears to be group 1-like in that it is in part duplicated with a group 1-like conserved loop sequence and is not group 2-like, as would be expected by the SARS coronavirus group 2-like 3' UTR structure. These results together indicate that stem-loop IV in the BCoV 5' UTR is a cis-acting element for DI RNA replication and that it might function through interactions with cellular proteins. It is postulated that stem-loop IV functions similarly in the virus genome.</div>
</front>
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