Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non‐human primates
Identifieur interne : 004B11 ( Main/Exploration ); précédent : 004B10; suivant : 004B12Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non‐human primates
Auteurs : Leo L. M. Poon [Hong Kong] ; Cynthia S. W. Leung [Hong Kong] ; Kwok H. Chan [Hong Kong] ; Kwok Y. Yuen [Hong Kong] ; Yi Guan [Hong Kong] ; Joseph S. M. Peiris [Hong Kong]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-08.
Descripteurs français
- KwdFr :
- ADN viral (), ADN viral (génétique), Adaptation biologique, Analyse de séquence d'ADN, Animaux, Cadres ouverts de lecture, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Haplorhini, Lignée cellulaire, Mutation, Passage en série, Protéines de l'enveloppe virale (génétique), Protéines de la matrice virale (génétique), Protéines nucléocapside (génétique), Réaction de polymérisation en chaîne, Substitution d'acide aminé, Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (croissance et développement), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- croissance et développement : Virus du SRAS.
- génétique : ADN viral, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- ADN viral, Adaptation biologique, Analyse de séquence d'ADN, Animaux, Cadres ouverts de lecture, Glycoprotéine de spicule des coronavirus, Haplorhini, Lignée cellulaire, Mutation, Passage en série, Réaction de polymérisation en chaîne, Substitution d'acide aminé.
- Pascal (Inist)
- Wicri :
- topic : Singe.
English descriptors
- KwdEn :
- Adaptation, Adaptation, Biological, Amino Acid Substitution, Animals, Cell Line, DNA, Viral (chemistry), DNA, Viral (genetics), Haplorhini, Isolation, Membrane Glycoproteins (genetics), Monkey, Mutation, Nucleocapsid Proteins (genetics), Open Reading Frames, Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (growth & development), SARS Virus (isolation & purification), Sequence Analysis, DNA, Serial Passage, Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Matrix Proteins (genetics).
- MESH :
- chemical , chemistry : DNA, Viral.
- chemical , genetics : DNA, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- genetics : SARS Virus.
- growth & development : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adaptation, Biological, Amino Acid Substitution, Animals, Cell Line, Haplorhini, Mutation, Open Reading Frames, Polymerase Chain Reaction, Sequence Analysis, DNA, Serial Passage, Spike Glycoprotein, Coronavirus.
Abstract
Four clinical isolates of SARS coronavirus were serially passaged in two primate cell lines (FRhK4 and Vero E6). Viral genetic sequences encoding for structural proteins and open reading frames 6–8 were determined in the original clinical specimen, the initial virus isolate (passage 0) and at passages 5, 10, and 15. After 15 passages, a total of 15 different mutations were identified and 12 of them were non‐synonymous mutations. Seven of these mutations were recurrent mutation and all located at the spike, membrane, and Orf 8a protein encoding sequences. Mutations in the membrane protein and a deletion in ORF 6–8 were already observed in passage 0, suggesting these amino acid substitutions are important in the adaptation of the virus isolate in primate cell culture. A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus. J. Med. Virol. 76:435–440, 2005. © 2005 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20379
Affiliations:
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Le document en format XML
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(isolation & purification)</term><term>Sequence Analysis, DNA</term><term>Serial Passage</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome virus</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Matrix Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral ()</term><term>ADN viral (génétique)</term><term>Adaptation biologique</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Haplorhini</term><term>Lignée cellulaire</term><term>Mutation</term><term>Passage en série</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines nucléocapside (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Substitution d'acide aminé</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Biological</term><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Haplorhini</term><term>Mutation</term><term>Open Reading Frames</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Serial Passage</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>Adaptation biologique</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Glycoprotéine de spicule des coronavirus</term><term>Haplorhini</term><term>Lignée cellulaire</term><term>Mutation</term><term>Passage en série</term><term>Réaction de polymérisation en chaîne</term><term>Substitution d'acide aminé</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Adaptation</term><term>Isolement</term><term>Mutation</term><term>Singe</term><term>Virus syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Singe</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four clinical isolates of SARS coronavirus were serially passaged in two primate cell lines (FRhK4 and Vero E6). Viral genetic sequences encoding for structural proteins and open reading frames 6–8 were determined in the original clinical specimen, the initial virus isolate (passage 0) and at passages 5, 10, and 15. After 15 passages, a total of 15 different mutations were identified and 12 of them were non‐synonymous mutations. Seven of these mutations were recurrent mutation and all located at the spike, membrane, and Orf 8a protein encoding sequences. Mutations in the membrane protein and a deletion in ORF 6–8 were already observed in passage 0, suggesting these amino acid substitutions are important in the adaptation of the virus isolate in primate cell culture. A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus. J. Med. Virol. 76:435–440, 2005. © 2005 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country></list><tree><country name="Hong Kong"><noRegion><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name></noRegion><name sortKey="Chan, Kwok H" sort="Chan, Kwok H" uniqKey="Chan K" first="Kwok H." last="Chan">Kwok H. Chan</name><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><name sortKey="Leung, Cynthia S W" sort="Leung, Cynthia S W" uniqKey="Leung C" first="Cynthia S. W." last="Leung">Cynthia S. W. Leung</name><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S. M." last="Peiris">Joseph S. M. Peiris</name><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S. M." last="Peiris">Joseph S. M. Peiris</name><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S. M." last="Peiris">Joseph S. M. Peiris</name><name sortKey="Yuen, Kwok Y" sort="Yuen, Kwok Y" uniqKey="Yuen K" first="Kwok Y." last="Yuen">Kwok Y. Yuen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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