SrasV1, Main, Exploration, bibRecord, 004946

A practical protocol for carbohydrate microarrays.

Identifieur interne : 004946 ( Main/Exploration ); précédent : 004945; suivant : 004947

A practical protocol for carbohydrate microarrays.

Auteurs : Ruobing Wang [États-Unis] ; Shaoyi Liu ; Dhaval Shah ; Denong Wang

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2005.

RBID : pubmed:16350957

Descripteurs français

KwdFr :
- Analyse par réseau de protéines (), Analyse par réseau de protéines (instrumentation), Animaux, Glucides (analyse), Humains, Oligosaccharides (analyse), Polyosides (), Séquençage par oligonucléotides en batterie (), Séquençage par oligonucléotides en batterie (instrumentation), Virus du SRAS ().
MESH :
- analyse : Glucides, Oligosaccharides.
- Analyse par réseau de protéines, Animaux, Humains, Polyosides, Séquençage par oligonucléotides en batterie, Virus du SRAS.

English descriptors

KwdEn :
- Animals, Carbohydrates (analysis), Humans, Oligonucleotide Array Sequence Analysis (instrumentation), Oligonucleotide Array Sequence Analysis (methods), Oligosaccharides (analysis), Polysaccharides (chemistry), Protein Array Analysis (instrumentation), Protein Array Analysis (methods), SARS Virus (chemistry).
MESH :
- chemical , analysis : Carbohydrates, Oligosaccharides.
- chemical , chemistry : Polysaccharides.
- chemistry : SARS Virus.
- instrumentation : Oligonucleotide Array Sequence Analysis, Protein Array Analysis.
- methods : Oligonucleotide Array Sequence Analysis, Protein Array Analysis.
- Animals, Humans.

Abstract

We have established a high-throughput biochip platform for constructing carbohydrate microarrays. Using this technology, carbohydrate-containing macromolecules of diverse structures, including polysaccharides, natural glycoconjugates, and mono- and oligosaccharides coupled to carrier molecules, can be stably immobilized on a glass chip without chemical modification. Here, we describe a practical protocol for this technology. We hope that anyone who has access to a standard cDNA microarray facility will be able to explore this technology for his or her own research interest. We also provide an example to illustrate that the carbohydrate microarray is also a discovery tool; this is particularly useful for identifying immunologic sugar moieties, including complex carbohydrates of cancer cells and sugar signatures of previously unrecognized microbial pathogens.

DOI: 10.1007/978-1-59259-948-6_17
PubMed: 16350957

Affiliations:

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<front><div type="abstract" xml:lang="en">We have established a high-throughput biochip platform for constructing carbohydrate microarrays. Using this technology, carbohydrate-containing macromolecules of diverse structures, including polysaccharides, natural glycoconjugates, and mono- and oligosaccharides coupled to carrier molecules, can be stably immobilized on a glass chip without chemical modification. Here, we describe a practical protocol for this technology. We hope that anyone who has access to a standard cDNA microarray facility will be able to explore this technology for his or her own research interest. We also provide an example to illustrate that the carbohydrate microarray is also a discovery tool; this is particularly useful for identifying immunologic sugar moieties, including complex carbohydrates of cancer cells and sugar signatures of previously unrecognized microbial pathogens.</div>
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Serveur d'exploration SRAS

A practical protocol for carbohydrate microarrays.

A practical protocol for carbohydrate microarrays.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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