A practical protocol for carbohydrate microarrays.
Identifieur interne : 004946 ( Main/Exploration ); précédent : 004945; suivant : 004947A practical protocol for carbohydrate microarrays.
Auteurs : Ruobing Wang [États-Unis] ; Shaoyi Liu ; Dhaval Shah ; Denong WangSource :
- Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- Animals, Carbohydrates (analysis), Humans, Oligonucleotide Array Sequence Analysis (instrumentation), Oligonucleotide Array Sequence Analysis (methods), Oligosaccharides (analysis), Polysaccharides (chemistry), Protein Array Analysis (instrumentation), Protein Array Analysis (methods), SARS Virus (chemistry).
- MESH :
- chemical , analysis : Carbohydrates, Oligosaccharides.
- chemical , chemistry : Polysaccharides.
- chemistry : SARS Virus.
- instrumentation : Oligonucleotide Array Sequence Analysis, Protein Array Analysis.
- methods : Oligonucleotide Array Sequence Analysis, Protein Array Analysis.
- Animals, Humans.
Abstract
We have established a high-throughput biochip platform for constructing carbohydrate microarrays. Using this technology, carbohydrate-containing macromolecules of diverse structures, including polysaccharides, natural glycoconjugates, and mono- and oligosaccharides coupled to carrier molecules, can be stably immobilized on a glass chip without chemical modification. Here, we describe a practical protocol for this technology. We hope that anyone who has access to a standard cDNA microarray facility will be able to explore this technology for his or her own research interest. We also provide an example to illustrate that the carbohydrate microarray is also a discovery tool; this is particularly useful for identifying immunologic sugar moieties, including complex carbohydrates of cancer cells and sugar signatures of previously unrecognized microbial pathogens.
DOI: 10.1007/978-1-59259-948-6_17
PubMed: 16350957
Affiliations:
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Le document en format XML
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<term>Glucides (analyse)</term>
<term>Humains</term>
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<term>Séquençage par oligonucléotides en batterie (instrumentation)</term>
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<front><div type="abstract" xml:lang="en">We have established a high-throughput biochip platform for constructing carbohydrate microarrays. Using this technology, carbohydrate-containing macromolecules of diverse structures, including polysaccharides, natural glycoconjugates, and mono- and oligosaccharides coupled to carrier molecules, can be stably immobilized on a glass chip without chemical modification. Here, we describe a practical protocol for this technology. We hope that anyone who has access to a standard cDNA microarray facility will be able to explore this technology for his or her own research interest. We also provide an example to illustrate that the carbohydrate microarray is also a discovery tool; this is particularly useful for identifying immunologic sugar moieties, including complex carbohydrates of cancer cells and sugar signatures of previously unrecognized microbial pathogens.</div>
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