Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Identifieur interne : 004876 ( Main/Exploration ); précédent : 004875; suivant : 004877Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Auteurs : Man Cheng [République populaire de Chine] ; Ceci W L. Chan ; Randy C F. Cheung ; Rama Kamesh Bikkavilli ; Qi Zhao ; Shannon W N. Au ; Paul K S. Chan ; Susanna S T. Lee ; Gregory Cheng ; Walter K K. Ho ; Wing-Tai CheungSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Anticorps antiviraux (immunologie), Données de séquences moléculaires, Interleukine-11 (immunologie), Lignée cellulaire, Protéines nucléocapside (), Protéines nucléocapside (immunologie), Rats, Réactions croisées (immunologie), Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Virus du SRAS (), Virus du SRAS (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Interleukine-11, Protéines nucléocapside, Réactions croisées, Virus du SRAS.
- Alignement de séquences, Animaux, Données de séquences moléculaires, Lignée cellulaire, Protéines nucléocapside, Rats, Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Base Sequence, Cell Line, Chlorocebus aethiops, Cross Reactions (immunology), Interleukin-11 (immunology), Mice, Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (immunology), Rats, SARS Virus (chemistry), SARS Virus (immunology), Sequence Alignment, Sequence Homology, Nucleic Acid.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Viral, Interleukin-11, Nucleocapsid Proteins.
- chemistry : SARS Virus.
- immunology : Cross Reactions, SARS Virus.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Mice, Molecular Sequence Data, Rats, Sequence Alignment, Sequence Homology, Nucleic Acid.
Abstract
Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.
DOI: 10.1016/j.bbrc.2005.10.088
PubMed: 16263078
Affiliations:
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Chan</name></author><author><name sortKey="Cheung, Randy C F" sort="Cheung, Randy C F" uniqKey="Cheung R" first="Randy C F" last="Cheung">Randy C F. Cheung</name></author><author><name sortKey="Bikkavilli, Rama Kamesh" sort="Bikkavilli, Rama Kamesh" uniqKey="Bikkavilli R" first="Rama Kamesh" last="Bikkavilli">Rama Kamesh Bikkavilli</name></author><author><name sortKey="Zhao, Qi" sort="Zhao, Qi" uniqKey="Zhao Q" first="Qi" last="Zhao">Qi Zhao</name></author><author><name sortKey="Au, Shannon W N" sort="Au, Shannon W N" uniqKey="Au S" first="Shannon W N" last="Au">Shannon W N. Au</name></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Lee, Susanna S T" sort="Lee, Susanna S T" uniqKey="Lee S" first="Susanna S T" last="Lee">Susanna S T. Lee</name></author><author><name sortKey="Cheng, Gregory" sort="Cheng, Gregory" uniqKey="Cheng G" first="Gregory" last="Cheng">Gregory Cheng</name></author><author><name sortKey="Ho, Walter K K" sort="Ho, Walter K K" uniqKey="Ho W" first="Walter K K" last="Ho">Walter K K. Ho</name></author><author><name sortKey="Cheung, Wing Tai" sort="Cheung, Wing Tai" uniqKey="Cheung W" first="Wing-Tai" last="Cheung">Wing-Tai Cheung</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Base Sequence</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cross Reactions (immunology)</term><term>Interleukin-11 (immunology)</term><term>Mice</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (chemistry)</term><term>Nucleocapsid Proteins (immunology)</term><term>Rats</term><term>SARS Virus (chemistry)</term><term>SARS Virus (immunology)</term><term>Sequence Alignment</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Données de séquences moléculaires</term><term>Interleukine-11 (immunologie)</term><term>Lignée cellulaire</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (immunologie)</term><term>Rats</term><term>Réactions croisées (immunologie)</term><term>Similitude de séquences d'acides nucléiques</term><term>Souris</term><term>Séquence nucléotidique</term><term>Virus du SRAS ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Interleukin-11</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Interleukine-11</term><term>Protéines nucléocapside</term><term>Réactions croisées</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cross Reactions</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Mice</term><term>Molecular Sequence Data</term><term>Rats</term><term>Sequence Alignment</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Lignée cellulaire</term><term>Protéines nucléocapside</term><term>Rats</term><term>Similitude de séquences d'acides nucléiques</term><term>Souris</term><term>Séquence nucléotidique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Au, Shannon W N" sort="Au, Shannon W N" uniqKey="Au S" first="Shannon W N" last="Au">Shannon W N. Au</name><name sortKey="Bikkavilli, Rama Kamesh" sort="Bikkavilli, Rama Kamesh" uniqKey="Bikkavilli R" first="Rama Kamesh" last="Bikkavilli">Rama Kamesh Bikkavilli</name><name sortKey="Chan, Ceci W L" sort="Chan, Ceci W L" uniqKey="Chan C" first="Ceci W L" last="Chan">Ceci W L. Chan</name><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name><name sortKey="Cheng, Gregory" sort="Cheng, Gregory" uniqKey="Cheng G" first="Gregory" last="Cheng">Gregory Cheng</name><name sortKey="Cheung, Randy C F" sort="Cheung, Randy C F" uniqKey="Cheung R" first="Randy C F" last="Cheung">Randy C F. Cheung</name><name sortKey="Cheung, Wing Tai" sort="Cheung, Wing Tai" uniqKey="Cheung W" first="Wing-Tai" last="Cheung">Wing-Tai Cheung</name><name sortKey="Ho, Walter K K" sort="Ho, Walter K K" uniqKey="Ho W" first="Walter K K" last="Ho">Walter K K. Ho</name><name sortKey="Lee, Susanna S T" sort="Lee, Susanna S T" uniqKey="Lee S" first="Susanna S T" last="Lee">Susanna S T. Lee</name><name sortKey="Zhao, Qi" sort="Zhao, Qi" uniqKey="Zhao Q" first="Qi" last="Zhao">Qi Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Cheng, Man" sort="Cheng, Man" uniqKey="Cheng M" first="Man" last="Cheng">Man Cheng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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