Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Identifieur interne : 004876 ( Main/Exploration ); précédent : 004875; suivant : 004877Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Auteurs : Man Cheng [République populaire de Chine] ; Ceci W L. Chan ; Randy C F. Cheung ; Rama Kamesh Bikkavilli ; Qi Zhao ; Shannon W N. Au ; Paul K S. Chan ; Susanna S T. Lee ; Gregory Cheng ; Walter K K. Ho ; Wing-Tai CheungSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Anticorps antiviraux (immunologie), Données de séquences moléculaires, Interleukine-11 (immunologie), Lignée cellulaire, Protéines nucléocapside (), Protéines nucléocapside (immunologie), Rats, Réactions croisées (immunologie), Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Virus du SRAS (), Virus du SRAS (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Interleukine-11, Protéines nucléocapside, Réactions croisées, Virus du SRAS.
- Alignement de séquences, Animaux, Données de séquences moléculaires, Lignée cellulaire, Protéines nucléocapside, Rats, Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Base Sequence, Cell Line, Chlorocebus aethiops, Cross Reactions (immunology), Interleukin-11 (immunology), Mice, Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (immunology), Rats, SARS Virus (chemistry), SARS Virus (immunology), Sequence Alignment, Sequence Homology, Nucleic Acid.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Viral, Interleukin-11, Nucleocapsid Proteins.
- chemistry : SARS Virus.
- immunology : Cross Reactions, SARS Virus.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Mice, Molecular Sequence Data, Rats, Sequence Alignment, Sequence Homology, Nucleic Acid.
Abstract
Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.
DOI: 10.1016/j.bbrc.2005.10.088
PubMed: 16263078
Affiliations:
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Le document en format XML
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<term>Antibodies, Viral (immunology)</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Cross Reactions (immunology)</term>
<term>Interleukin-11 (immunology)</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Rats</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (immunology)</term>
<term>Sequence Alignment</term>
<term>Sequence Homology, Nucleic Acid</term>
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<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Données de séquences moléculaires</term>
<term>Interleukine-11 (immunologie)</term>
<term>Lignée cellulaire</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Rats</term>
<term>Réactions croisées (immunologie)</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Souris</term>
<term>Séquence nucléotidique</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Nucleocapsid Proteins</term>
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<term>Protéines nucléocapside</term>
<term>Réactions croisées</term>
<term>Virus du SRAS</term>
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<term>SARS Virus</term>
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<term>Base Sequence</term>
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<term>Chlorocebus aethiops</term>
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<term>Molecular Sequence Data</term>
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<term>Sequence Homology, Nucleic Acid</term>
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<front><div type="abstract" xml:lang="en">Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.</div>
</front>
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<name sortKey="Cheung, Randy C F" sort="Cheung, Randy C F" uniqKey="Cheung R" first="Randy C F" last="Cheung">Randy C F. Cheung</name>
<name sortKey="Cheung, Wing Tai" sort="Cheung, Wing Tai" uniqKey="Cheung W" first="Wing-Tai" last="Cheung">Wing-Tai Cheung</name>
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