SrasV1, Main, Exploration, bibRecord, 004025

The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory

Identifieur interne : 004025 ( Main/Exploration ); précédent : 004024; suivant : 004026

The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory

Auteurs : Timothy M. Barkham [Singapour] ; Youne Kow Chung [Singapour] ; Kin Fai Tang [Singapour] ; Eng Eong Ooi [Singapour]

Source :

Transactions of The Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ] ; 2006.

RBID : ISTEX:B7136F7C3671FE7BEEAA8BABAECCF274885737FC

Descripteurs français

KwdFr :
- ARN viral (isolement et purification), Audit médical, Dengue (diagnostic), Dengue (virologie), Diagnostic différentiel, Humains, RT-PCR (normes), Sensibilité et spécificité, Singapour, Test ELISA (normes), Virus de la dengue (isolement et purification).
MESH :
- diagnostic : Dengue.
- isolement et purification : ARN viral, Virus de la dengue.
- normes : RT-PCR, Test ELISA.
- virologie : Dengue.
- Audit médical, Diagnostic différentiel, Humains, Sensibilité et spécificité, Singapour.
Wicri :
- geographic : Singapour.

English descriptors

KwdEn :
- Dengue (diagnosis), Dengue (virology), Dengue Virus (isolation & purification), Diagnosis, Differential, Enzyme-Linked Immunosorbent Assay (standards), Humans, Medical Audit, RNA, Viral (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (standards), Sensitivity and Specificity, Singapore.
MESH :
- chemical , isolation & purification : RNA, Viral.
- geographic : Singapore.
- diagnosis : Dengue.
- isolation & purification : Dengue Virus.
- standards : Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Dengue.
- Diagnosis, Differential, Humans, Medical Audit, Sensitivity and Specificity.

Abstract

The laboratory diagnosis of dengue has largely relied on serological assays, although many different RT-PCR protocols have been reported. Owing to its limited use, the value of RT-PCR in the clinical laboratory has not been fully evaluated. During the outbreak of severe acute respiratory syndrome (SARS) in Singapore in 2003, RT-PCR to detect dengue viral RNA was used as a rapid diagnostic tool to differentiate dengue from SARS among patients who presented to a hospital designated to manage and quarantine SARS cases. A total of 343 results for RT-PCR and 439 results for serology were analysed and compared with the final discharge diagnosis. Our experience indicates that RT-PCR for dengue can be set up rapidly in a clinical laboratory, with very sensitive and specific results for the diagnosis of dengue, particularly in the first 5 days from onset of symptoms.

Url:

DOI: 10.1016/j.trstmh.2005.05.015

Affiliations:

Singapour

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<front><div type="abstract">The laboratory diagnosis of dengue has largely relied on serological assays, although many different RT-PCR protocols have been reported. Owing to its limited use, the value of RT-PCR in the clinical laboratory has not been fully evaluated. During the outbreak of severe acute respiratory syndrome (SARS) in Singapore in 2003, RT-PCR to detect dengue viral RNA was used as a rapid diagnostic tool to differentiate dengue from SARS among patients who presented to a hospital designated to manage and quarantine SARS cases. A total of 343 results for RT-PCR and 439 results for serology were analysed and compared with the final discharge diagnosis. Our experience indicates that RT-PCR for dengue can be set up rapidly in a clinical laboratory, with very sensitive and specific results for the diagnosis of dengue, particularly in the first 5 days from onset of symptoms.</div>
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Serveur d'exploration SRAS

The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory

The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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