Analysis of multiplex PCR fragments with PMMA microchip.
Identifieur interne : 003F85 ( Main/Exploration ); précédent : 003F84; suivant : 003F86Analysis of multiplex PCR fragments with PMMA microchip.
Auteurs : Dayu Liu [République populaire de Chine] ; Xiaomian Zhou ; Runtao Zhong ; Nannan Ye ; Guohui Chang ; Wei Xiong ; Xiaodan Mei ; Bingcheng LinSource :
- Talanta [ 1873-3573 ] ; 2006.
Abstract
Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products.
DOI: 10.1016/j.talanta.2005.04.064
PubMed: 18970365
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A39
- to stream PubMed, to step Curation: 001A39
- to stream PubMed, to step Checkpoint: 002300
- to stream Ncbi, to step Merge: 001D78
- to stream Ncbi, to step Curation: 001D78
- to stream Ncbi, to step Checkpoint: 001D78
- to stream Main, to step Merge: 004198
- to stream Main, to step Curation: 003F85
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of multiplex PCR fragments with PMMA microchip.</title>
<author><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian</wicri:regionArea>
<wicri:noRegion>Dalian</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Xiaomian" sort="Zhou, Xiaomian" uniqKey="Zhou X" first="Xiaomian" last="Zhou">Xiaomian Zhou</name>
</author>
<author><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name>
</author>
<author><name sortKey="Ye, Nannan" sort="Ye, Nannan" uniqKey="Ye N" first="Nannan" last="Ye">Nannan Ye</name>
</author>
<author><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name>
</author>
<author><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name>
</author>
<author><name sortKey="Mei, Xiaodan" sort="Mei, Xiaodan" uniqKey="Mei X" first="Xiaodan" last="Mei">Xiaodan Mei</name>
</author>
<author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:18970365</idno>
<idno type="pmid">18970365</idno>
<idno type="doi">10.1016/j.talanta.2005.04.064</idno>
<idno type="wicri:Area/PubMed/Corpus">001A39</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A39</idno>
<idno type="wicri:Area/PubMed/Curation">001A39</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A39</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002300</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002300</idno>
<idno type="wicri:Area/Ncbi/Merge">001D78</idno>
<idno type="wicri:Area/Ncbi/Curation">001D78</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001D78</idno>
<idno type="wicri:Area/Main/Merge">004198</idno>
<idno type="wicri:Area/Main/Curation">003F85</idno>
<idno type="wicri:Area/Main/Exploration">003F85</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of multiplex PCR fragments with PMMA microchip.</title>
<author><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian</wicri:regionArea>
<wicri:noRegion>Dalian</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Xiaomian" sort="Zhou, Xiaomian" uniqKey="Zhou X" first="Xiaomian" last="Zhou">Xiaomian Zhou</name>
</author>
<author><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name>
</author>
<author><name sortKey="Ye, Nannan" sort="Ye, Nannan" uniqKey="Ye N" first="Nannan" last="Ye">Nannan Ye</name>
</author>
<author><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name>
</author>
<author><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name>
</author>
<author><name sortKey="Mei, Xiaodan" sort="Mei, Xiaodan" uniqKey="Mei X" first="Xiaodan" last="Mei">Xiaodan Mei</name>
</author>
<author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name>
</author>
</analytic>
<series><title level="j">Talanta</title>
<idno type="eISSN">1873-3573</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><noCountry><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name>
<name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name>
<name sortKey="Mei, Xiaodan" sort="Mei, Xiaodan" uniqKey="Mei X" first="Xiaodan" last="Mei">Xiaodan Mei</name>
<name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name>
<name sortKey="Ye, Nannan" sort="Ye, Nannan" uniqKey="Ye N" first="Nannan" last="Ye">Nannan Ye</name>
<name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name>
<name sortKey="Zhou, Xiaomian" sort="Zhou, Xiaomian" uniqKey="Zhou X" first="Xiaomian" last="Zhou">Xiaomian Zhou</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003F85 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003F85 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:18970365 |texte= Analysis of multiplex PCR fragments with PMMA microchip. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18970365" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |