Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains.
Identifieur interne : 003D00 ( Main/Exploration ); précédent : 003C99; suivant : 003D01Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains.
Auteurs : Karen H Nel [Allemagne] ; Thomas Stangler ; Matthias Stoldt ; Dieter WillboldSource :
- Journal of biomedical science [ 1021-7770 ] ; 2006.
Descripteurs français
- KwdFr :
- Antigène-1 associé à la fonction du lymphocyte (), Antigènes CD (), Cadres ouverts de lecture, Clonage moléculaire, Données de séquences moléculaires, Humains, Immunoglobulines (), Interleukine-1 (), Intégrines (), Modèles moléculaires, Molécule-1 d'adhérence intercellulaire (), Molécules d'adhérence cellulaire (), Protéines de la matrice virale (), Protéines virales (), Structure secondaire des protéines, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS ().
- MESH :
- Antigène-1 associé à la fonction du lymphocyte, Antigènes CD, Cadres ouverts de lecture, Clonage moléculaire, Données de séquences moléculaires, Humains, Immunoglobulines, Interleukine-1, Intégrines, Modèles moléculaires, Molécule-1 d'adhérence intercellulaire, Molécules d'adhérence cellulaire, Protéines de la matrice virale, Protéines virales, Structure secondaire des protéines, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antigens, CD (chemistry), Cell Adhesion Molecules (chemistry), Cloning, Molecular, Humans, Immunoglobulins (chemistry), Integrins (chemistry), Intercellular Adhesion Molecule-1 (chemistry), Interleukin-1 (chemistry), Lymphocyte Function-Associated Antigen-1 (chemistry), Models, Molecular, Molecular Sequence Data, Open Reading Frames, Protein Structure, Secondary, Protein Structure, Tertiary, SARS Virus (chemistry), Viral Matrix Proteins (chemistry), Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : Antigens, CD, Cell Adhesion Molecules, Immunoglobulins, Integrins, Intercellular Adhesion Molecule-1, Interleukin-1, Lymphocyte Function-Associated Antigen-1, Viral Matrix Proteins, Viral Proteins.
- chemistry : SARS Virus.
- Amino Acid Sequence, Cloning, Molecular, Humans, Models, Molecular, Molecular Sequence Data, Open Reading Frames, Protein Structure, Secondary, Protein Structure, Tertiary.
Abstract
The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectroscopy. The fold of the protein is the first member of a further variation of the immunoglobulin like beta-sandwich fold. Because X4 does not reveal significant sequence homologies to proteins in the data bases, we carried out a structure based similarity search for proteins with known function. High structural similarity to Dl domains of ICAM-1 and ICAM-2, and common features in amino acid sequence between X4 and ICAM-1, suggest X4 to possess binding activity for the alpha(L) integrin I domain of LFA-1. Further, based on this structure based prediction, potential functions of X4 in virus replication and pathogenesis are discussed.
DOI: 10.1007/s11373-005-9043-9
PubMed: 16328780
Affiliations:
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Le document en format XML
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Informationsverarbeitung (IBI-2), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Forschungszentrum Jülich, Institut für Biologische Informationsverarbeitung (IBI-2)</wicri:regionArea><wicri:noRegion>Institut für Biologische Informationsverarbeitung (IBI-2)</wicri:noRegion><wicri:noRegion>Institut für Biologische Informationsverarbeitung (IBI-2)</wicri:noRegion><wicri:noRegion>Institut für Biologische Informationsverarbeitung (IBI-2)</wicri:noRegion></affiliation></author><author><name sortKey="Stangler, Thomas" sort="Stangler, Thomas" uniqKey="Stangler T" first="Thomas" last="Stangler">Thomas Stangler</name></author><author><name sortKey="Stoldt, Matthias" sort="Stoldt, Matthias" uniqKey="Stoldt M" first="Matthias" last="Stoldt">Matthias Stoldt</name></author><author><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name></author></analytic><series><title level="j">Journal of biomedical science</title><idno type="ISSN">1021-7770</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antigens, CD (chemistry)</term><term>Cell Adhesion Molecules (chemistry)</term><term>Cloning, Molecular</term><term>Humans</term><term>Immunoglobulins (chemistry)</term><term>Integrins (chemistry)</term><term>Intercellular Adhesion Molecule-1 (chemistry)</term><term>Interleukin-1 (chemistry)</term><term>Lymphocyte Function-Associated Antigen-1 (chemistry)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>SARS Virus (chemistry)</term><term>Viral Matrix Proteins (chemistry)</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigène-1 associé à la fonction du lymphocyte ()</term><term>Antigènes CD ()</term><term>Cadres ouverts de lecture</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Immunoglobulines ()</term><term>Interleukine-1 ()</term><term>Intégrines ()</term><term>Modèles moléculaires</term><term>Molécule-1 d'adhérence intercellulaire ()</term><term>Molécules d'adhérence cellulaire ()</term><term>Protéines de la matrice virale ()</term><term>Protéines virales ()</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, CD</term><term>Cell Adhesion Molecules</term><term>Immunoglobulins</term><term>Integrins</term><term>Intercellular Adhesion Molecule-1</term><term>Interleukin-1</term><term>Lymphocyte 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matrice virale</term><term>Protéines virales</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectroscopy. The fold of the protein is the first member of a further variation of the immunoglobulin like beta-sandwich fold. Because X4 does not reveal significant sequence homologies to proteins in the data bases, we carried out a structure based similarity search for proteins with known function. High structural similarity to Dl domains of ICAM-1 and ICAM-2, and common features in amino acid sequence between X4 and ICAM-1, suggest X4 to possess binding activity for the alpha(L) integrin I domain of LFA-1. Further, based on this structure based prediction, potential functions of X4 in virus replication and pathogenesis are discussed.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Stangler, Thomas" sort="Stangler, Thomas" uniqKey="Stangler T" first="Thomas" last="Stangler">Thomas Stangler</name><name sortKey="Stoldt, Matthias" sort="Stoldt, Matthias" uniqKey="Stoldt M" first="Matthias" last="Stoldt">Matthias Stoldt</name><name sortKey="Willbold, Dieter" sort="Willbold, Dieter" uniqKey="Willbold D" first="Dieter" last="Willbold">Dieter Willbold</name></noCountry><country name="Allemagne"><noRegion><name sortKey="H Nel, Karen" sort="H Nel, Karen" uniqKey="H Nel K" first="Karen" last="H Nel">Karen H Nel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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