[Identification of mimotope peptides which bind to the SARS-CoV spike protein specific monoclonal antibody 2C5 with phage-displayed peptides library].
Identifieur interne : 003C16 ( Main/Exploration ); précédent : 003C15; suivant : 003C17[Identification of mimotope peptides which bind to the SARS-CoV spike protein specific monoclonal antibody 2C5 with phage-displayed peptides library].
Auteurs : Rong-Hong Hua ; Dong-Lai Wu ; Guang-Zhi Tong ; Yun-Feng Wang ; Zhi-Jun Tian ; Yan-Jun ZhouSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2006.
Descripteurs français
- KwdFr :
- Anticorps monoclonaux (immunologie), Banque de peptides, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Séquence d'acides aminés, Test ELISA, Virus du SRAS (immunologie), Épitopes.
- MESH :
- immunologie : Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- Banque de peptides, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Séquence d'acides aminés, Test ELISA, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Monoclonal (immunology), Enzyme-Linked Immunosorbent Assay, Epitopes, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Molecular Sequence Data, Peptide Library, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : SARS Virus.
- Amino Acid Sequence, Enzyme-Linked Immunosorbent Assay, Epitopes, Molecular Sequence Data, Peptide Library, Spike Glycoprotein, Coronavirus.
Abstract
To identify the epitope of SARS-CoV spike protein specific neutralizing monoclonal antibody (MAb) 2C5. The antibody was used as target and three rounds of bio-panning were conducted with phage-display peptide library. After the third panning, 20 phage-plague clones were randomly picked and analyzed for the binding ability with the MAb 2C5 by ELISA. The display sequence analysis demonstrated that among the twenty phage clones, eight clones displayed the same seven-peptide TPEQQFT. All these eight phage-clones showed strongest binding activity with 2C5 in phage ELISA analysis. Furthermore, phages displaying peptide TPEQQFT could specifically inhibit the binding of MAb 2C5 with SARS-CoV spike protein. The results demonstrated that TPEQQFT is a mimic epitope peptide containing neutralizing MAb 2C5. This study may provide information for further structural and functional analysis of spike protein and development vaccine for severe acute respiratory syndrome.
DOI: 10.1016/s1872-2075(06)60051-4
PubMed: 17037189
Affiliations:
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Le document en format XML
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first="Yun-Feng" last="Wang">Yun-Feng Wang</name></author><author><name sortKey="Tian, Zhi Jun" sort="Tian, Zhi Jun" uniqKey="Tian Z" first="Zhi-Jun" last="Tian">Zhi-Jun Tian</name></author><author><name sortKey="Zhou, Yan Jun" sort="Zhou, Yan Jun" uniqKey="Zhou Y" first="Yan-Jun" last="Zhou">Yan-Jun Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17037189</idno><idno type="pmid">17037189</idno><idno type="doi">10.1016/s1872-2075(06)60051-4</idno><idno type="wicri:Area/PubMed/Corpus">002066</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002066</idno><idno type="wicri:Area/PubMed/Curation">002066</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002066</idno><idno type="wicri:Area/PubMed/Checkpoint">001F06</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F06</idno><idno 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Science</wicri:noCountry></affiliation></author><author><name sortKey="Wu, Dong Lai" sort="Wu, Dong Lai" uniqKey="Wu D" first="Dong-Lai" last="Wu">Dong-Lai Wu</name></author><author><name sortKey="Tong, Guang Zhi" sort="Tong, Guang Zhi" uniqKey="Tong G" first="Guang-Zhi" last="Tong">Guang-Zhi Tong</name></author><author><name sortKey="Wang, Yun Feng" sort="Wang, Yun Feng" uniqKey="Wang Y" first="Yun-Feng" last="Wang">Yun-Feng Wang</name></author><author><name sortKey="Tian, Zhi Jun" sort="Tian, Zhi Jun" uniqKey="Tian Z" first="Zhi-Jun" last="Tian">Zhi-Jun Tian</name></author><author><name sortKey="Zhou, Yan Jun" sort="Zhou, Yan Jun" uniqKey="Zhou Y" first="Yan-Jun" last="Zhou">Yan-Jun Zhou</name></author></analytic><series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title><idno type="ISSN">1000-3061</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Monoclonal (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux (immunologie)</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (immunologie)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Séquence d'acides aminés</term><term>Test ELISA</term><term>Virus du SRAS (immunologie)</term><term>Épitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Séquence d'acides aminés</term><term>Test ELISA</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To identify the epitope of SARS-CoV spike protein specific neutralizing monoclonal antibody (MAb) 2C5. The antibody was used as target and three rounds of bio-panning were conducted with phage-display peptide library. After the third panning, 20 phage-plague clones were randomly picked and analyzed for the binding ability with the MAb 2C5 by ELISA. The display sequence analysis demonstrated that among the twenty phage clones, eight clones displayed the same seven-peptide TPEQQFT. All these eight phage-clones showed strongest binding activity with 2C5 in phage ELISA analysis. Furthermore, phages displaying peptide TPEQQFT could specifically inhibit the binding of MAb 2C5 with SARS-CoV spike protein. The results demonstrated that TPEQQFT is a mimic epitope peptide containing neutralizing MAb 2C5. This study may provide information for further structural and functional analysis of spike protein and development vaccine for severe acute respiratory syndrome.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Hua, Rong Hong" sort="Hua, Rong Hong" uniqKey="Hua R" first="Rong-Hong" last="Hua">Rong-Hong Hua</name><name sortKey="Tian, Zhi Jun" sort="Tian, Zhi Jun" uniqKey="Tian Z" first="Zhi-Jun" last="Tian">Zhi-Jun Tian</name><name sortKey="Tong, Guang Zhi" sort="Tong, Guang Zhi" uniqKey="Tong G" first="Guang-Zhi" last="Tong">Guang-Zhi Tong</name><name sortKey="Wang, Yun Feng" sort="Wang, Yun Feng" uniqKey="Wang Y" first="Yun-Feng" last="Wang">Yun-Feng Wang</name><name sortKey="Wu, Dong Lai" sort="Wu, Dong Lai" uniqKey="Wu D" first="Dong-Lai" last="Wu">Dong-Lai Wu</name><name sortKey="Zhou, Yan Jun" sort="Zhou, Yan Jun" uniqKey="Zhou Y" first="Yan-Jun" last="Zhou">Yan-Jun Zhou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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